Combined Antioxidant Recovery Properties of Fruit Juice Extracts of Cucumber and Water Melon on Lipid Profile and Gonadal Steroid Levels of Cadmium Induced Testicular Damage on Male Albino Rats

Main Article Content

F. C. Anacletus
B. Nwakaku
K. T. Nwauche

Abstract

The antioxidant protective effects of fruit juice of cucumber and watermelon on lipid profile of cadmium induced toxicity on male albino rats was investigated. Forty male rats were divided into eight groups. Group NC served as normal control group while group PC was positive control that was not treated but induced with cadmium. Groups I to VI received high dose and low dose of juice of Cucumber and Watermelon respectively. Excluding the normal control group, other groups were fed with lard 14 days before treatment commenced.  Doses of 0.8 mg/kg-high dose and 0.4 mg/kg-low dose for cucumber and watermelon respectively. At the 4th and 6th week, biochemical parameters were assayed. Results revealed that the levels of total cholesterol, LDL, VLDL and triglyceride significantly (P˂0.05) were decreased compared to positive control but HDL was increased in treatment groups compared to positive control. Pretreatment with cucumber and watermelon juice indicated that total cholesterol, LDL, VLDL and triglyceride significantly (P˂0.05) were decreased compared to positive control but HDL was increased in treatment groups compared to positive control. The result also revealed an increase in testosterone levels in treated groups after 4 weeks of administration of whole extract of cucumber and watermelon when compared to their week 2 values. Testosterone level in positive control was also reduced significantly from 1.5±0.14 ng/ml to 0.46±0.31 ng/ml. Histological evaluation of the testes of normal control group revealed that the interstitium was intact with leydig cells present and maturing germ cells embedded in normal seminiferous tubules while the other groups that were induced with cadmium only showed morphology of testes with empty seminiferous tubules and consolidated interstitial spaces.

Keywords:
Cadmium, water melon, cucumber, lipid, toxicity

Article Details

How to Cite
Anacletus, F. C., Nwakaku, B., & Nwauche, K. T. (2019). Combined Antioxidant Recovery Properties of Fruit Juice Extracts of Cucumber and Water Melon on Lipid Profile and Gonadal Steroid Levels of Cadmium Induced Testicular Damage on Male Albino Rats. Asian Journal of Research in Biochemistry, 4(4), 1-9. https://doi.org/10.9734/ajrb/2019/v4i430076
Section
Original Research Article

References

Samarghandian S, Borji A, Tabasi SH. Eff ects of Cichorium intybus linnon blood glucose, lipid constituents and selected oxidative stress parametersin streptozotocin-induced diabetic rats. Cardiovascular Hematological Disorder Drug Targets. 2013;13:231–236.

Benoff S, Jacob A, Hurley IR. Male infertility and environmental exposure to lead and cadmium. Human Reproduction Update. 2000;6(2):107-121.

Jarup L. Cadmium overload and toxicity. Nephrology Dialysis Transplant. 2002;2: 35–39.

Järup L. Hazards of heavy metal contamination. British Medical Bulletin. 2003;68(1):167-182.

WHO. Heavy metals and toxicity: Health criteria and other supporting information. World Health Organisation, Geneva. 2010;7:84-90.

Roccheri MC, Tipia C, Bonaventura R, Matranga V (). Physiological and induced apoptosis in sea urchin larvae undergoing metamorphosis. International Journal of Developmental Biology. 2004;46(6):801-806.

Järup L, Hellström L, Alfvén T, Carlsson M. D, Grubb A, Persson B, Elinder CG. Low level exposure to cadmium and early kidney damage: the OSCAR study. Occupational and Environmental Medicine. 2000;57(10):668-672.

Yu HN, Shen SR, Yin JJ. Effects of metal ions, catechins, and their interactions on postrate cancer. Critical Review in Food Science and Nutrition. 2007;47(8):711-9.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress- induced cancer. Chemico - Biological Interactions.160:1-40. Weast RC (1974). Handbook of Chemistry and Physics, 55th ed., Cleveland, Ohio, CRC Press. 2006; 500.

Oh SH, Lim SC. A rapid and transient ROS generation by cadmium triggers apoptosis via caspase-dependent pathway in HepG2 cells and this is inhibited through N-acetylcysteine - mediated catalase upregulation. Toxicology and Applied Pharmacology. 2006;212(3):212-223.

Khorasani AM, Cheung AP, Lee CYG. Cholesterol inhibitory effects on human sperm-induced acrosome reaction. Journal of Andrology. 2000;21(4):586-594.

Shimamoto K, Sofikitis N. Effect of hypercholesterolemia ontesticular function and sperm physiology. YonagoActa. Medica. 1998;41:23-29.

Tandon Sk, Singh S, Prasad S, Khandekar K, Dwivedi VK, Chatterjee M, Mathur N. Reversal of cadmium induced oxidative stress by chelating agent, antioxidant or their combination in rat. Toxicology Letters. 2003;145:211-7.

Gupta RS, Gupta ES, Dhakal BK, Thakur AR, Ahnn J. Vitamin C and Vitamin Е protect the rat testes from cadmium-induced reactive oxygen species. Molecular Cells. 2004;17(1):132-139.

Kara H, Karatas F, Canatan H, Servi K. Effects of exogenous metallothionein on acute cadmium toxicity in rats. Biological Trace Element Research. 2005;104(3): 223-232.

Cai D. NFĸB-mediated metabolic inflammation inperpheral tissues versus central nervous system. Cell Cycle. 2009;8:2542-2548.

Valdecantos MP, Pérez-Matute P, Martinez JA. Obesity and oxidative stress: Role of antioxidant supplementation. Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 2008;61(2):127-139.

Marnett LJ. Lipid peroxidation—DNA damage by malondialdehyde. Mutation Research / Fundamental and Molecular Mechanisms of Mutagenesis. 1999;424 (1):83-95.

Penuel BL, Khan EM, Maitera MO. Properties of proximate composition and Elementary Analysis of Citrullus Vulgaris (Guna) seed. Bulletin of Environmental, Pharmacology and Life Science. 2013;2 (2):39-46.

Anacletus Francis, Nwakaku Beauty, Nwauche Kelechi, Monago-Ighorodje Comfort. Effect of combined fruit juice of Citrullus lanatus and Cucumis sativus on Cadmium Induced Hepatotoxicity and Nephrotoxicity in Male Wister Rats. Scholars Academic Journal of Biosciences(SAJB). 2017;5(10):732-743.

Nonnecke IL. Vegetable production. Springer Science and Business Media; 1989.

Georgina EO, Kingsley O, Esosa US, Helen NK, Frank AO, Anthony AC. Comparartive evaluation of antioxidant effects of watermelon and orange, and their effects on some serum lipid profile of Wistar albino rats. International Journal of Nutrition and Metabolism. 2011;3(8):97-102.

Anacletus FC, Onuah CL, Nwauche KT. The effect of combined ethanol leaf extracts of Annona muricata and Artocarpus heterophyllus on Reproductive parameters of Type 2 diabetic Wistar albino rats. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2019;6(4):1-8.

Heidari B, Pessarakli M, Dadkhodaie A, Daneshnia N. Reactive oxygen species-mediated functions in plants under environmental stresses. Journal of Agricultural Science and Technology. 2012; B2(2B):159.

Wu CR, Huang MY, Lin YT, Ju HY, Ching H. Antioxidant properties of Cortex Fraxini and its simple coumarins. Food Chemistry. 2007;104(4):1464-1471.

Nawal KA, Ula AK, Ban TS. Protective effect of zinc on reproductive parameters in male rat treated with cadmium. American Journal of Medical Science. 2015;5:73-81.

Obianime AW, Roberts II. Antioxidants, cadmium - induced toxicity, serum biochemical and the histological abnormalities of the kidney and testes of the male Wistar rats. Nigerian Journal of Physiological Sciences. 2009;24(2):303-8.

Adaikpoh MA, Obi FO. Prevention of cadmium-induced alteration in rat testes and prostate lipid patterns by-tocopherol. African Journal of Biochemistry Research. 2009;3(10):321-325.