Qualitative and Quantitative Phytochemical Screening, Antioxidant and Anti-inflammatory Activities of Acetone Extract of Brassica juncea L. Leaf

Main Article Content

Pratima Khandayataray
Meesala Krishna Murthy


Objective: The aim of this study is to evaluate the phytochemical screening; antioxidant and antiinflammatory activity of acetone extract of Brassica juncea (L.) Czern leaf.

Methods: Qualitative and quantitative phytochemical screening was conducted by following standard protocols. To assess the antioxidant properties, Nitric oxide (NO) assay, 1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'- Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid, ABTS), Protease inhibitor assay, anti-inflammatory and reducing power (%) activities were performed by using the standard methodology.

Results: Qualitative investigation showed the presence of alkaloids, carbohydrates, phenols, flavonoids, proteins, saponins and tannins respectively. Quantitative analysis showed protein content was more followed by phenolic content, glucose concentration, total antioxidant activity and flavonoid content respectively. The inhibitory concentration (IC50) and EC50 values along with standards were estimated by using NO, DPPH and ABTS respectively. Protease inhibitor (%) and reducing power (%) activities were increased dose dependent manner, at higher dose 1000µg/mL the Protease inhibitor (%) and reducing power (%) activities 537.95 ± 26.58 (%) and 41.28 ± 7.28 were observed respectively.

Conclusion: Based on our results, we concluded that acetone extracts of B.juncea leaf showed potential antioxidant and anti-inflammatory properties.

Brassica juncea L. leaf, acetone extract, phytochemicals, antioxidants, anti-inflammatory

Article Details

How to Cite
Khandayataray, P., & Murthy, M. K. (2019). Qualitative and Quantitative Phytochemical Screening, Antioxidant and Anti-inflammatory Activities of Acetone Extract of Brassica juncea L. Leaf. Asian Journal of Research in Biochemistry, 5(1), 1-15. https://doi.org/10.9734/ajrb/2019/v5i130078
Original Research Article


Subedi L, Timalsena S, Duwadi P, Thapa R, Paudel A, Parajuli K. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J Trad Chin Med. 2014;34(5):584-590.

Magalhães LM, Barreiros L, Reis S, Segundo MA. Kinetic matching approach applied to ABTS assay for high-throughput determination of total antioxidant capacity of food products. J Food Comp Anal. 2014;33(2):187-194.

Sevindik M. Investigation of anti-oxidant/oxidant status and antimicrobial activities of Lentinus tigrinus. Adv Pharmacol Sci. 2018;1718025.

Huang CY, Wu SJ, Yang WN, Kuan AW, Chen CY. Antioxidant activities of crude extracts of fucoidan extracted from Sargassum glaucescens by a compressional-puffing-hydrothermal extraction process. Food Chem. 2016; 197(Pt B):1121-1129.

Deng J, Cheng W, Yang G. A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chem. 2011;125(4):1430-1435.

Sevindik M. Investigation of oxidant and antioxidant status of edible mushroom Clavariadelphus truncatus. The Journal of Fungus. 2018;9(2):165-168.

Subedi L, Timalsena S, Duwadi P, Thapa R, Paudel A, Parajuli K. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. J Trad Chin Med. 2014;34(5):584-590.

Siddique AB, Rahman SMM, Hossain MA, Rashid MA. Phytochemical screening and comparative antimicrobial potential of different extracts of Stevia rebaudiana Bertoni leaves. Asian Pac J Trop Dis. 2014;4:275-280.

Valavala VK, Vangipurapu RK, Banam VR, Pulukurthi UMR, Turlapati NR. Effect of mustard (Brassica juncea) leaf extract on streptozotocin-induced diabetic cataract in wistar rats. J Food Biochem. 2011;35(1): 109-124.

Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull. 2017;26: 4757-4763.

Mishra A, Pragyandip D, Murthy P, Hh S, Kushwaha P. A classical review on Rajika (Brassica juncea). Res Rev J Bot Sci. 2012;1:18-23.

Kim HY, Yokozawa T, Cho EJ, Cheigh HS, Choi JS, Chungh HY. In vitro and in vivo antioxidant effects of mustard leaf (Brassica juncea). Phytotherapy Research. 2003;17:465-471.

Kumar V, Thakur AK, Barothia ND, Chatterjee SS. Therapeutic potentials of Brassica juncea: An overview. Int J Genuine Tradit Med. 2011;1:e2.

Li J, Ho CT, Li H, Tao H, Tao L. Separation of sterols and triterpene alcohols from unsaponifiable fractions of three plant seed oils. J Food Lipids. 2000;7:11-20.

Yokozawa T, Kim HY, Cho EJ, Choi JS, Chung HY. Antioxidant effects of Isorhamnetin 3, 7-di-O-beta-D-glucopyranoside isolated from mustard leaf (Brassica juncea) in rats with streptozotocin-induced diabetes. J Agric Food Chem. 2002;50:5490-5495.

Das R, Bhattacherjee C, Ghosh S. Preparation of mustard (Brassica juncea L.) protein isolate and recovery of phenolic compounds by ultrafiltration. Ind Eng Chem Res. 2009;48:4939-4947.

Jung HA, Woo JJ, Jung MJ, Hwang GS, Choi JS. Kaempferol an update on Brassica juncea glycosides with antioxidant activity from Brassica juncea. Arch Pharm Res. 2009;32:1379-1384.

Ufelle SA, Ukaejiofo EO, Neboh EE, Achukwu PU, Ghasi S, Ikekpeazu JE, Maduka IC. The effects of crude methanol seed extract of "Brassica juncea" on haematological parameters in wistar rats. British Journal of Pharmacology and Toxicology. 2011;2(3):123-126.

Gupta A, Naraniwal M, Kothari V. Modern extraction methods for preparation of bioactive plant extracts. International Journal of Applied and Natural Sciences. 2012;1(1):8-26.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685-688.

Raman N. Phytochemical techniques. Chapter 5 qualitative phytochemical screening. New India Publishing Agency, Pitampura, New Delhi. 2006;19-24.

Takaidza S, Mtunzi F, Pillay M. Analysis of the phytochemical contents and antioxidant activities of crude extracts from Tulbaghia species. J Tradit Chin Med. 2018;38(2):272-279.

Karamian R, Ghasemlou F. Screening of total phenol and flavonoid content, antioxidant and antibacterial activities of the methanolic extracts of three Silene species from Iran. Int J Agric Crop Sci. 2013;5(3):305-312.

Kallel F, Driss D, Chaari F, Belghith L, Bouaziz F, Ghorbel R, Chaabouni SE. Garlic (Allium sativum L.) husk waste as a potential source of phenolic compounds: Influence of extracting solvents on its antimicrobial and antioxidant properties. Ind Crops and Prod. 2014;62:34-41.

Parikh H, Khanna A. Pharmacognosy and phytochemical analysis of Brassica juncea seeds. Pharmacognosy Journal. 2014;6(5): 47-54.

Howlader MM, Ahmed SR, Kubra K, Bhuiyan MK. Biochemical and phyto-chemical evaluation of Stevia rebaudiana. Asian J Med Biol Res. 2016;2(1):121-130.

Paumorad F, Hosseineiemehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afric J Biotechnol. 2006;5(11):1142-1145.

Folin O, Ciocalteu V. On tyrosine and tryptophane determination in proteins. J Biol Chem. 1927;27:627-650.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193:265-275.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-341.

Alam MB, Hossain MS, Haque ME. Antioxidant and anti-inflammatory activities of the leaf extract of Brassica nigra. International Journal of Pharmaceutical Sciences and Research. 2011;2(2):303.

Alisi CS, Onyeze GOC. Nitric oxide scavenging ability of ethyl acetate fraction of methanolic leaf extracts of Chromolaena odorata (Linn.). African Journal of Biochemical Research. 2008;2(7):145-150.

Wong SP, Leong LP, Koh JHW. Antioxidant activities of aqueous extracts of selected plants. Food Chem. 2006;99: 775-783.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radica cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-1237.

Kumarappan CT, Chandra R, Mandal SC. Anti-inflammatory activity of Ichnocarpus frutescens. Pharmacology. 2006;3:201-216.

Oyedapo OO, Famurewa AJ. Antiprotease and membrane stabilizing activities of extracts of Fagara Zanthoxyloides, Olax subscorpioides and Tetrapleura tetraptera. International Journal of Pharmacognosy. 1995;33:65-69.

Oyaizu M. Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Japanese Journal of Nutrition. 1986;44:307-315.

Ahmed MF, Rao AS, Ahemad SR, Ibrahim MO. Phytochemical studies and antioxidant activities of Brassica oleracea L. Var. Capitata. Int J Pharm Pharm Sci. 2012;4(3):374-378.

SPSS Inc. Released 2009. PASW Statistics for Windows, Version 18.0. Chicago: SPSS Inc.

Singh A, Singh K, Saxena A. Hypoglycaemic activity of different extracts of various herbal plants. Int J Res Ayurveda Pharm. 2010;1(1):212-224.

Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sc. 1999;65(4):337-353.

López-Cobo A, Gómez-Caravaca AM, Švarc-Gajic´ J, Segura-Carretero A, Fernández-Gutiérrez A. Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii. J Funct Foods. 2015;18:1167-1178.

Chen X. Protective effects of quercetin on liver injury induced by ethanol. Pharmacogn Mag. 2010;6:135-141.

Bentz AB. A review of quercetin: Chemistry, antioxidant properties, and bioavailability. J Young Investig. 2009;19: 1-8.

Cartea ME, Francisco M, Soengas P, Velasco P. Phenolic compounds in Brassica vegetables. Molecules. 2010;16: 251-280.

Brock A, Herzfeld T, Paschke R, Koch M, Drager B. Brassicaceae contain nortropane alkaloids. Phytochemistry. 2006;67:2050-2057.

Zou Z, Xi W, Hu Y, Nie C, Zhou Z. Antioxidant activity of Citrus fruits. Food Chem. 2016;196(1):885-896.

Soyingbe OS, Ayedeji AO, Basson AK, Singh M, Opoku AR. Chemical composition, antimicrobial and antioxidant properties of the essential oils of Tulbaghia violacea Harv L.F. Afric J Microbiol Res. 2013;7(18):1787-1793.

Han SS, Lo SC, Choi YW, Kim JH, Baek SH. Antioxidant activity of crude extract and pure compounds of Acer ginnala (Max.) Bull Korean Chem Soc. 2004;25(3): 389-391.

Cottrell GS, Amadesi S, Schmidlin F, Bunnett N. Protease-activated receptor 2: Activation, signaling and function. Biochem Soc Trans. 2003;31:1191-1197.

Stefano F, Eleonora D. Proteinase-activated receptors (PARs) and immune function. Drug Dev Res. 2003;60:65-70.

Parellada J, Guinea M. Flavonoid inhibitors of trypsin and leucine aminopeptidase: A proposed mathematical model for IC50 estimation. J Nat Prod. 1995;58(6):823-829.

Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40:945-948.

Yang RY, Lin S, Kuo G. Content and distribution of fl avonoids among 91 edible plant species. Asia Pac J Clin Nutr. 2008;17(1):275-279.