In-vitro Biochemical Studies on Organometallic Compounds as Anticancer Agents

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F. Z. Mohammed
Elsherbiny H. Elsayed
Atef E. Abd Elbaky
H. M. Shalaby


This study aims to synthesis of copper complexes of 2,3-dihydroxy benzaldehyde thiosemicarbazone (3a,b), followed by evaluating their in vitro anticancer properties. The prepared compounds have been also evaluated for their ability to induce apoptosis. A total number of 80 adult female Swiss albino mice weighing 20-25 gm were divided randomly into 8 groups (10 mice /each group). The in vitro cytotoxic activities of compounds (3a, 3b) were evaluated. Minimum inhibitory concentrations of synthesized compound 3a were found to be 50 μg/mL against MCF-7, HepG2 and PC3 cell lines; also, Minimum inhibitory concentrations of synthesized compound 3b were found to be 50 μg/mL in all cell lines. The apoptotic effect of compounds 3a and 3b were evaluated by measurement Caspase-3 activity and Bcl-2 concentration. The mean values of Caspase-3 activity in positive control were found to be 2.6151 (ng/mL). On the other hand, the mean values of Bcl-2 in positive control were found to be 3.7 (ng/mL). The compounds (3a & 3b) exhibited a significant anticancer activity towards MCF-7, HEPG2 and PC3 cancer cell lines.

In vitro anticancer properties, copper complexes, 2,3-dihydroxy benzaldehyde thiosemicarbazone, adult female Swiss albino mice, MCF-7, HepG2 and PC3 cell lines.

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Mohammed, F., Elsayed, E., Elbaky, A. E. A., & Shalaby, H. (2019). In-vitro Biochemical Studies on Organometallic Compounds as Anticancer Agents. Asian Journal of Research in Biochemistry, 5(1), 1-9.
Original Research Article


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