In-vivo Study on Organometallic Compounds as Anticancer Agents

Main Article Content

F. Z. Mohammed
Elsherbiny H. Elsayed
Atef E. Abd Elbaky
H. M. Shalaby


This study aims to study the in-vivo anticancer effect of the synthesized copper complexes of 2,3-dihydroxy benzaldehyde thiosemicarbazone (3a,b), followed by evaluating their antioxidant activity.  Materials and methods: A total number of 80 adult female swiss albino mice weighing 20-25 gm were divided into 8 groups (10 mice /each group).  The acute toxicity was estimated by intraperitoneal injection of the compounds (3a, b). Results:  We found that, 5 mg /kg and 10 mg /kg were considered to be the most effective dose of compounds 3a & 3b, respectively. The mean volume of EAC in the positive control group was found to be 4.2 ±0.5 (mL), this value was significantly decreased by 100%, (p<0.001) for 3a & 3b treated groups, respectively.

Anticancer, copper complexes, 2,3-dihydroxy benzaldehyde thiosemicarbazone, Erhlich Ascites Carcinoma (EAC), swiss albino mice.

Article Details

How to Cite
Mohammed, F. Z., Elsayed, E. H., Elbaky, A. E. A., & Shalaby, H. M. (2019). In-vivo Study on Organometallic Compounds as Anticancer Agents. Asian Journal of Research in Biochemistry, 5(1), 1-8.
Original Research Article


Tmamm AS, Mohammed FZ, EL-Deen IM. Cytotoxicity of N-(P-chlorophenyl)-7- hydroxycoumarin -3-yl carboxamide and Ethyl 7-hydroxycoumarin -3-yl Ester. Asian Journal of Research in Biochemistry AJRB. 2019;1(2):49084.

World Health Organization: Cancer Facts & Figures. Latest global cancer data, Cancer burden rises to 18.1 million new cases and 9.6 million cancer deaths in 2018. World Health Organization, Geneva, Switzerland; 2018.

National cancer control plans: A global analysis. Published in the Lancet Oncology. Global Cancer Control; 2018.

Zahran F, Keshta AT, EL-Deen IM, El-behary MM. Mode of action of potassium salt of 2-thioxo-4-hydroxycoumarin [3, 4-b] pyrimidine and 9-bromo-2-thioxo-hydroxy-coumarin [3, 4-b] pyrimidine against Ehrlich Ascites carcinoma cells. Biol. Chem. Res. 2014;76-8.

Kane A, Yang I. Interferon-gamma in brain tumor immunotherapy. Neurosurg. Clin. N. Am. 2010;21:77–86.

Jackson P, Bartek J. The DNA-damage response in human biology and disease. Nature. 2009;461(7267):1071-1078.

Khan A, Nazar H, Sabir S, Irshad M, Awan S, Abbas R, Akram M, khaliq A, Rocha J, Ahmad S, Farnaz Malik F. Antioxidant activity and inhibitory effect of some commonly used medicinal plants against lipid per-oxidation in mice brain asmatullah. 2014;11(5):83-90.

Meier J, Theakston RD. Approximate LD50 determinations of snake venoms using eight to ten experimental animals. Toxicon. 1986;24:395–401.

Crump S, Hoel G, Langley H, Peto R. Fundamental carcinogenic processes and their implications for low dose risk assessment. Cancer Research. 1976;36: 2973–2979.

McLiman F, Dairs V, Glover L, Rake W. The submerged culture of mammalian cells: The Spinner Culture. J. Immunol. 1957;79:428-433.

Thavasu W, Longhurst S, Joel P, Slevin L, Balkwill R. Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions. J Immunol Methods. 1992;153(12):115-124.

Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta. 1978;90:37-43.

Aebi H. Catalase in vitro, Methods Enzymol. 1984;6:105-121.

Levesque R, SPSS. Programming and data management: A guide for SPSS and SAS users. Fourth Edition, SPSS Inc., Chicago Ill; 2007.

Helleday T, Petermann E, Lundin C, Hodgson B, Sharma A. DNA repair pathways as targets for cancer therapy. Nat Rev Cancer. 2008;8(3):193-204.

Jakupec A, Galanski M, Arion V, Hartinger G, Keppler K. Antitumour metal compounds: More than theme and variations. Dalton Transactions. 2008;2: 183-194.

Arora S, Agarwal S, Singhal S. Anticancer activities of thiosemicarbazides/ thiosemicarbazones. Int J Pharm Pharm Sci. 2014;6(9):34-41.

Reed G, Poyner R, Larsen T, Wedekind J, Raymenta I. Structural and mechanistic studies of enolase, Current Opinion in Structural Biology. 1996;6(6):736-743.

Sathisha A, Budagumpi S, Kulkarni N, Gurunath S, Kurdekar A, Revankar A, Pai K. Synthesis, structure, electrochemistry and spectral characterization of (D-glucopyranose)-4-phenylthiosemicarbazide metal complexes and their antitumor activity against Ehrlich Ascites Carcinoma in Swiss albino miceq M.P, European Journal of Medicinal Chemistry. 2010; 45(3):106–113.

Al-Amin M, Akhter S, Hasan T, Alam T, Nageeb M, Saifullah R, Shohel M. The antioxidant effect of astaxanthin is higher in young mice than aged: A region specific study on brain. Metab Brain Dis. 2015; 30(5):1237-1246.

Hajhashemi V, Vaseghi G, Pourfarzam M, Abdollahi A. Are antioxidants helpful for disease prevention? Res Pharm Sci. 2010; 5(1):1–8.

Peruma P, Chamuah J, Rajkhowa D, Chandan C. Effect of catalase on the liquid storage of mithun (Bos frontalis) semen. Asian Pacific Journal of Reproduction. 2013;2(5):209–214.

Rebecca F, Bruce A. In comprehensive natural products II; 2010.

Thanh N, Hoai L. Synthesis, structure and antioxidant activity of (TetraO-acetyl-?-D-galactopyranosyl) thiosemicarbazones of substituted benzaldehydes. Indian J Pharm Sci. 2012;74(1):54-62.