Amelioration of Monosodium Glutamate-induced Testicular Damage and Infertility in Male Rats by Water Melon and Cantaloupe Seeds Extract and Juices

Main Article Content

Shimaa El-Masry
Mai Elsayed

Abstract

Aims: Monosodium glutamate (MSG) is extensively used as food additive and flavor enhancer, there is a growing concern that this may affect the male reproductive system and fertility. The objective of this study is to investigate the effect of MSG on fertility and testes of mature male rats and the ameliorative role of water melon and cantaloupe (seeds extract and juices).

Study Design: Thirty-six male Sprague - Dawely rats (150-180 g) were randomly assigned into six groups (n=6). Group (1): orally administered with distilled water. Group (2): orally administered with 60 mg/kg of MSG. Groups (3 and 4): orally administered with 60 mg/kg of MSG + 200 mg/kg of water melon seeds extract and juice respectively. Groups (5 and 6): orally administered with 60 mg/kg of MSG + 200 mg/kg of cantaloupe seeds extract and juice respectively.

Results: Results showed that administration of MSG for 6 weeks caused abnormalities of semen characteristics, increased DNA damage and up-regulation of caspase3 expression in the testes tissue. Also, the levels of plasma sex hormones were decreased and the oxidant-antioxidant status was disturbed, moreover, MSG caused alteration in the histopathological structures of testicular tissue. Administration of seeds extract or juices of water melon and cantaloupe almost corrected the biochemical and histopathological alteration produced by MSG.

Conclusion: This study concluded that water melon and cantaloupe seeds and juice extracts have an ameliorative role against MSG-induced testicular damage and infertility in rats.

Keywords:
Monosodium glutamate, testes, male infertility, watermelon, cantaloupe, Citrullus lanatus, Cucumis melo L., antioxidant.

Article Details

How to Cite
El-Masry, S., & Elsayed, M. (2019). Amelioration of Monosodium Glutamate-induced Testicular Damage and Infertility in Male Rats by Water Melon and Cantaloupe Seeds Extract and Juices. Asian Journal of Research in Biochemistry, 5(2), 1-16. https://doi.org/10.9734/ajrb/2019/v5i230088
Section
Original Research Article

References

Hamza RZ, AL-Harbi MS. Monosodium glutamate induced testicular toxicity and the possible ameliorative role of vitamin E or selenium in male rats. Toxicology. 2014;1:1037–1045.

Niaz K, Zaplatic E, Spoor J. Extensive use of monosodium glutamate: A threat to public health? EXCLI Journal. 2018;17: 273-278.

Kadir RE, Omotoso GO, Balogun TJ, Oyetunji AO. Effects of monosodium glutamate on semen quality and the cytoarchitecture of the testis of adult wistar rats. Int. J. Biomed. & Hlth. Sci. 2011;7(1): 39-46.

Abd-Ella EMM, Mohammed AMA. Attenuation of monosodium glutamate-induced hepatic and testicular toxicity in albino rats by Annona muricata Linn. (Annonaceae) leaf extract. Journal of Pharmacy and Biological Sciences. 2016;11(6):61-69.

Kuldip S, Ahluwalia P. Studies on the effect of MSG administration on some antioxidant enzymes in arterial tissue of adult male mice. Journal of Nutrition Sciences and Vitaminology. 2003;49:145-148.

Eweka O. Histological studies of the effects of monosodium glutamate on the kidney of adult Wistar rats. Internet J Health. 2007;6:2-8.

Vinodini NA, Nayanatara A, Damodar G, Damodar B, Ramaswamy CR, Shabarinath S, Bhat MR. Effect of monosodium induced oxidative damage on rat testis. J Clin Med. 2010;3:370–373.

Alalwani AD. Monosodium glutamate induced testicular lesionsin rats (histological study). Middle East Fertility Society Journal. 2014;19:274–280.

Gill NS, Bajwa J, Dhiman K, Sharma P, Sood S, Sharma PD, Singh B, Bali M. Evaluation of therapeutic potential of traditionally consumed Cucumis melo seeds. Asian Journal of Plant Sciences. 2011;10(1):86-91.

Addo PW, Agbenorhevi JK, Adu-Poku D. Antinutrient contents of watermelon seeds. MOJ Food Processing and Technology. 2018;6(2):237-239.

Koocheki V, Razavi SMA, Milani E, Moghadam TM, Abedini M, Alamatiyan S, Izadkhah S. Physical properties of watermelon seed as a function of moisture content and variety. Int. Agrophysics. 2007;21:349-359.

Perkins-Veazie P, Collins JK, Pair SD, Roberts W. Lycopene content differs among red-fleshed watermelon cultivars. J. Sci. Food Agric. 2001;81(10):983–987.

Alok BRK, Vivek D, Niyaz A. Evaluation of anti-ulcer activity of Citrallus lanatus seed extract in Wistar rats. J. Pharm. Pharm. Sci. 2012;4(5):0975-1491.

Oyenihia OR, Afolabia BA, Oyenihib AB, Ogunmokuna OJ, Oguntibejuca OO. Hepato- and neuro-protective effects of watermelon juice on acuteethanol-induced oxidative stress in rats. Toxicology Reports. 2013;3:288–294.

Oyenihia OR, Afolabia BA, Oyenihib AB, Ogunmokuna OJ, Oguntibeju OO. Hepato- and neuro-protective effects of watermelon juice on acuteethanol-induced oxidative stress in rats. Toxicology Reports. 2016;3: 288–294.

Naz A, Butt MS, Sultan MT, Qayyum MMN, Niaz RS. Watermelon lycopene and allied health claims. EXCLI Journal. 2014;13:650-666.

Zeb A. Phenolic profile and antioxidant activity of melon (Cucumis melo L.) seeds from Pakistan. Foods. 2016;5(67):1-7.

Mehra M, Pasricha V, Gupta RK. Estimation of nutritional, phytochemical and antioxidant activity of seeds of musk melon (Cucumis melo) and water melon (Citrullus lanatus) and nutritional analysis of their respective oils. Journal of Pharmacognosy and Phytochemistry. 2015;3(6):98-102.

Vella FM, De Masi L, Laratta B. Antioxidant activity of cantaloupe (Cucumis melo L.) peel extract. Natural Products in Health, Agro-Food and Cosmetics; 2017.

Adesanya AO, Olasinde OO, Oguntayo OD, Otuluana JO, Adefule AK. Effects of methanolic effect of Citrillus lanatus seed on experimentally induced prostatic hyperplasia. European Journal of Medicinal plants. 2011;1(4):171–179.

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988;175:184-191.

Ohkawa H, Ohishi W, Yogi K. Anal. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. J. Biochem. 1979;95:351-358.

Teitze F. Enzymatic method for quantitative determination of nanogram amount of total and oxidized glutathione: Application to mammalian and other tissue. Anal. Biochem. 1969;27:502-255.

Bancroft JD, Stevens A, Turner DR. Theory and practice of histological techniques, 4th Ed. Livingstone, New Yourk, Churchill; 1996.

Levesque R. SPSS programming and data management: A guide for SPSS and SAS user. 3rd Edition, United States of America; 2007.

Moore KL. Congenital malformations due to environment in developing humans. 2nd Ed. Philadelphia, W.B. Saunders co. Ltd. 2003;173-183.

Farombi EO. Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: modulatory role of Vitamin C, Vitamin E and Quercetin. Human and Experimental Toxicology. 2006;25:251-259.

Vinodini NA, Nayanatara AK, Ramaswamy C, Ranade AV, Rheka DK, Damadara GKM, et al. Study on evaluation of monosodium glutamate-induced oxidative damage on renal tissue on adult wistar rats. Journal of Chinese Clinical Medicine. 2010;5(3).

Ismail NH. Assessment of DNA damage in testes from young wistar male rat treated with monosodium glutamate. Life Science Journal. 2012;9(1):930-939.

Onakewhor JUE, Oforofuo IAO, Singh SP. Chronic administration of monosodium glutamate induces oligozoospermia and glycogen accumulation in Wistar rat testes. Afri J. Reprod. Health. 1998;2(2):190-197.

Oforofuo IAO, Onakewhor JUE, Idaewor PE. The effect of chronic administration of MSG on the histology of the adult Wistar rat testes. Bioscience Research Communication. 1997;9(2).

Takarada TE, hinoi VJ, Balcar H, Taniura Y. Possible of functional glutarnate transporters in the rat testis. J. Endocrinol. 2004;181:233-244.

Giovambattista A, Suescun MO, Nessralla CC, Franca LR, Spinedi E, Calandra RS. Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocinology. 2003;78:270-279.

Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: Relationship with semen quality. J Androl. 2000;21:33-44.

Robinson MB. Acute regulation of sodium-dependent glutamate transporters: A focus on constitutive and regulated trafficking. Handb. Exp. Pharmacol. 2006;175:251–275.

Print CG, Loveland KL. Germ cell suicide. New insights into apoptosis during spermatogenesis. Bioessays. 2000;22: 423–30.

Vardi N, Parlakpinar H, Ates B, Cetin A, Otlu A. Antiapoptotic and antioxidant effects of carotene against methotrexate-induced testicular injury. Int. J. Fertil Steril. 2009;92(6):2028-2033.

Sarhan NR. The ameliorating effect of sodium selenite on the histological changes and expression of Caspase-3 in the testis of monosodium glutamate-treated rats: Light and electron microscopic study. J Microsc Ultrastruct. 2018;6(2): 105–115.

Wang JM, Xiao BL, Zheng JW, Chen HB, Zou SQ. Effect of targeted magnetic nanoparticles containing 5 FU on expression of bcl 2, bax and caspase 3 in nude mice with transplanted human liver cancer. World J Gastroenterol. 2007;13(23):3171 3175.

McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb. Perspect. Biol. 2013;5: a008656.

Wang R, Yeh S, Tzeng C, Chang C. Androgen receptor roles in spermatogenesis and fertility: Lessons from testicular cell-specifi c androgen receptor knockout mice. Endocrinol. Rev. 2009;30:119-132.

Babu SR, Sadhnani MD, Swarna M, Padmavathi P, Reddy PP. Evaluation of FSH, LH and testosterone levels in different subgroups of infertile males. Indian Journal of Clinical Biochemistry. 2004;19(1):45-49.

Zabul J, Mierzejewski W, Rogoza A. Usefulness of examining gonadotropin hormones and testosterone in men with abnormal semen. Ginekol-pol. 1994;65(2): 71-74.

Sakr SA, Badawi GM. protective effect of curcumin on monosodium glutamate-induced reproductive toxicity in male albino rats. Global Journal of Pharmacology. 2013;7(4):416-422.

Frank L, Suescun M, Miranda J, Giovambattista A, Perello M, Spinedi E, Calandra R. Testis structure and function in a nongenetic hyperadipose rat model at prepubertal and adult ages. Endocinology. 2006;147:1556-1563.

Boodnard IP, Gooz H, Okamura BE, et al. Effect of neonatal treatment with monosodium glutamate on dopaminergic neurons of hypothalamus and on prolactin secretion of rats. Brain Res. Bull. 2001;55:767-774.

Hu J, Zhang Z, Shen WJ, Azhar S. Cellular cholesterol delivery, intra cellular processing and utilization for biosynthesis of steroid hormones. Nutr. Metab. 2010;7: 47-72.

Ongjanovic LBI, Markovic L, Dor.d-evic SD, Trbojevic NZ, Tajn ISA, Saicic LZS. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q10 and Vitamin E, Reprod. Toxicol. 2010;29:191–197.

Onyema OO, Farombi EO, Emerole GO, Ukoha AI, Onyeze GO. Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats. Indian J Biochem Biophys. 2006;43:20– 4.

Ozyurt H, Sog˘ut S, Yıldırım Z, Kart L, Iraz M, Armutc, et al. Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats. Clin. Chim. Acta. 2004;339:65–75.

Sener G, Sehirli AO, Ayanoglu-Dulger G. Melatonin protects against mercury (II)-induced oxidative tissue damage in rats. Pharmacol. Toxicol. 2003;93:290–296.

Reiner W. Middlebury Natural Foods Cooperative, Dedicated to the Health and Wellbeing of the Whole Community. MNFC Newsletter A Monthly Publication of the Middlebury Natural Foods Cooperative; 2009.

Mohammed AI, Al-Bayati MA. Comparative study among watermelon crud extract, citrulline and lycopene on some reproductive indices in male mice. International Journal of Research in Medical and Health Sciences. 2014;3(6):7-14.

Godspower O, Williams NK, Elochukwu N. Ameliorative potential of methanolic extract of Citrullus lanatus (Watermelon) seeds on the sperm parameters, testosterone level and testicular cytoarchitecture of male albino rats induced with lead-acetate. British Journal of Pharmaceutical Research. 2015;6(1):35-43.

Khaki A, Fathiazad F, Nouri M. Effects of watermelon seed extract (Citrullus vulgaris) on spermatogenesis in rat. Int J Women’s Health Reproduction Sci. 2013;1(3):99-104.

Li F, Xu XR, Li S, Deng GF, Wu S, Li HB. Resources and bioactivities of lycopene. Int J Food Nutr Safety. 2012;1:15–31.

Deng GF, Xu XR, Li S, Li F, Xia EQ, Li HB. Natural sources and bioactivities of resveratrol. Int J Modern Biol Med. 2012;1: 1–20.

Rolim PM, Fidelis GP, Padilha CEA, Santos ES, Rocha HAO, Macedo GR. Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells. Brazilian Journal of Medical and Biological Research. 2018;51(4).

Arora R, Kaur M, Gill NS. Antioxidant activity and pharmacological evaluation of Cucumis melo var. agrestis methanolic seed extract. Research Journal of Phytochemistry .2011;5(3):146-155.

Daramola OO, Oyeyemi WA, Beka FU, Ofutet EA. Protective effects of aqueous extract of Citrullus lanatus fruit on reproductive functions and antioxidant activities in arsenic-treated male wistar rats. Afr. J. Biomed. Res. 2018;21:65-72.

Narmada PG, Kumar R. Lycopene therapy in idiophatic male infertility – a preliminary. Report. International Urology and Nephrology. 2002;34:369-372.

Sadek K, Abouzed T, Nasr S. Lycopene modulates cholinergic dysfunction, Bcl-2/Bax balance, and antioxidant enzymes gene transcripts in monosodium glutamate (E621) induced neurotoxicity in a rat model Can. J. Physiol. Pharmacol. 2016;94:1-8.

Dokumacioglu E, Iskender H, Yenice G, Kapakin KAT, Sevim CA, Hayirli A, et al. Effects of astaxanthin on biochemical and histopathological parameters related to oxidative stress on testes of rats on high fructose regime. WILEY International Journal of Andrologi. 2018;1:1-10.

Chaturvedi P, Pipedi-Tshekiso M, Tumed A. Supplementation with watermelon renders protection against toxicity induced by paracetamol in albino rats: The mutual and fine interaction of antioxidants prevented the cellular damage. Int. J. Food Agric. Vet. Sci. 2014;1:102–111.

Choudhary BR, Haldhar SM, Maheshwari SK, Bhargava R, Sharma SK. Phytochemicals and antioxidants in watermelon (Citrullus lanatus) genotypes under hot arid region. Indian J Agric Sci. 2015;85(3):414-7.

Alalwani AD. Monosodium glutamate induced testicular lesions in rats (histological study). Middle East Fertility Society Journal. 2014;19:274–280.

Khayal EEH, Mohammed ZAM, Ahmed MM. Influence of selenium on mono sodium glutamate as food additive induced hepatotoxicity and testicular toxicity in adult albino rats. Egypt J. Forensic Sci. Appli. Toxicol. 2018;18(3):1-22.

Mendes TB, Paccola CC, Neves FMO, Simas JN, Vaz AC, Regina. Resveratrol improves reproductive parameters of adult rats varicocelized in peripuberty. Reproduction. 2016;152:23–35.

Oliveira FA, Costa WS, Sampaio FJB, Gregorio BM. Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates. Asian Journal of Andrology. 2018;20:1–7.