Evaluation of the Antioxidant Activity of Stalk and Fruit of Solanum aethiopicum L. (Solanaceae)

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Kady Diatta
William Diatta
Alioune Dior Fall
Serigne Ibra Mbacké Dieng
Amadou Ibrahima Mbaye
Abdou Sarr
Mourchidath Adjoke Adegbindin


Background: The use of plants for healing dates back to very remote times. Nowadays with the accession of new diseases plants are increasingly used for the formulation of new drugs able to overcome the many diseases (cancer, atherosclerosis) often caused by the disorder of the system prooxidant/antioxidant.

Aim/Objective: On the strength of this observation, the research of an antioxidant plant is essential, hence the aim of this study, which is to determine the antioxidant activity of the stalk and the fruit of Solanum aethiopicum L.

Methods: The fruits and stalk were washed, cut into fine slats, then dried in the incubator for three days and finally crushed into powder. An extraction by decoction with ethanol (stalks and fruits) and water (fruit) was subsequently carried out to obtain three extracts (ethanol and water). Antioxidant activity was evaluated through the FRAP method, and the trapping of radical DPPH.

Results: For the FRAP method, at the highest concentration (1 mg/ml) the aqueous extract of the fruit (74.84±2.97%) has a higher reducing power compared to those of the ethanolic extracts of the fruit (70.15 ± 5.72%) and the stalk (49.85 ± 2.11%). These reducing powers, although significant, remain lower than those of tannic acid (89.95±0.007%). And finally, for the DPPH method, the aqueous extract of the fruit is more effective in reducing free radical DPPH with a IC50= 162±33 µg/ml, follow up by ethanol extract from the stalk (IC50= 360± 90 µg/ml) and finally ethanol extract from the fruit (IC50= 362.5± 23.5 µg/ml). These results confirm the in vitro antioxidant activity of the studied parts of Solanum aethiopicum.

Conclusion: Prospective studies could focus on acute and subacute toxicities and the determination of the molecules responsible for the activity.

Stalks, fruit, Solanum aethiopicum, antioxidant activity, DPPH, FRAP.

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How to Cite
Diatta, K., Diatta, W., Dior Fall, A., Mbacké Dieng, S. I., Ibrahima Mbaye, A., Sarr, A., & Adjoke Adegbindin, M. (2020). Evaluation of the Antioxidant Activity of Stalk and Fruit of Solanum aethiopicum L. (Solanaceae). Asian Journal of Research in Biochemistry, 6(1), 6-12. https://doi.org/10.9734/ajrb/2020/v6i130107
Original Research Article


Benbrinis S. Evaluation des activités antioxydante et antibactérienne des extraits de Santolina
chamaecyparissus. Thèse de Magister en Biochimie, Université de SETIF. 2012;84.

Meziti A. Activité antioxydante des extraits des graines de Nigella sativa L. Étude in vitro et in vivo.
Thèse de Magister en Biochimie Appliquée, Université de Batna. 2008;105.

Diamanti J, Battino M, Mezzetti B. Breeding for fruit nutritional and nutraceutical quality. In Breeding for Fruit Quality;
Jenks, M. A., Bebeli, P., Eds.; John Wiley & Sons: Hoboken, NJ, USA. 2011;61–80.

Alarcón-Flores MI, Romero-González R, Martínez Vidal JL, Egea González FJ, Garrido Frenich A. Monitoring of
phytochemicals in fresh and fresh-cut vegetables: A comparison. Food Chem. 2014;142:392–399.
DOI: 10.3390/antiox4020345

Burgos-Morón E, Calderón-Montaño JM, Orta ML, Pastor N, Pérez-Guerrero C, Austin C, Mateos S, López-Lázaro M. The coffee constituent chlorogenic acid induces cellular DNA damage and formation of topoisomerase I- and II-DNA complexes in cells. J. Agric. Food Chem. 2012;60:7384–7391.
DOI: 10.1021/jf300999e

Ahn EH, Kim DW, Shin MJ, Kwon SW, Kim YN, Kim DS, Lim SS, Kim J, Park J, Eum WS, Hwang HS, Choi SY. Chlorogenic acid improves neuroprotective effect of PEP-1-ribosomal protein S3 against ischemic insult. Exp. Neurobiol. 2011;20:169–175.
DOI: 10.5607/en.2011.20.4.169

Bassene E. Initiation à la recherché sur les substances naturelles: Extraction – Analyses – Essais biologiques. Presses Universitaires de Dakar. 2012;150.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Techno. 2003;26:211-219.

Ogunka-Nnoka CU, Onyegeme-Okerenta BM, Omeje HC. Effects of ethanol extracts of S. aethiopicum
stalks on lipid profile and haematological parameters of wistar albino rats. International Journal of Science a
nd Research Methodology. 2018;10(3):215-229.

Stommel JR, Whitaker BD. Phenolic acid content and composition of eggplant fruit in a germplasm core subset.
J. Am. Soc. Hortic. Sci. 2003;128:704–710.

Sunseri F, Polignano GB, Alba V, Lotti C, Bisignano V, Mennella G, D’alessandro A, Bacchi M, Riccardi P, Fiore MC
and Ricciardi L. Genetic diversity and characterization of African eggplant germplasm collection. Afr. J. Plant Sci.
ISSN: 1996-0824. 2010;4:231–241.

Mariola P, Prohens J, Cuñat An, Vilanova S, Gramazio P, Herraiz FJ, Andujar I. Reducing capacity, chlorogenic acid content and
biological activity in a collection of scarlet (Solanum aethiopicum) and Gboma (S. macrocarpon) eggplants.
International Journal of Molecular Sciences. 2014;15(10):17221-17242.
DOI: 10.3390/ijms151017221

Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma P. A review of phytochemistry and pharmacology
of flavonoids. Internationale Pharmaceutica Sciencia. 2011;1(1):25-41.

Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat. Rev. Cancer. 2003;3:768–780.
DOI: 10.1038/nrc1189

Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic
agents: Mechanism and action. Mutat. Res. 2005;579:200–213.
DOI: 10.1016/j.mrfmmm.2005.03.023

Mbaye AI, Gueye PM, Fall AD, Kane MO, Diatta KB, Sarr A, Diattara D, Bassene E. Antioxidative activity of Tamarindus indica L.
extract and chemical fractions. African Journal of Biochemistry Research. ISSN: 1996-0778. 2017;11(2):6-11.
DOI: 10.5897/AJBR2016.0896

Dieng SIM, Fall AD, Diatta KB, Sarr A, Sene M, Mbaye AI, Diatta W, Bassene E. Evaluation de l’activité antioxydante des extraits
hydro-ethanoliques des feuilles et écorces de Piliostigma thonningii Schumach. Int. J. Biol. Chem. Sci. ISSN: 1997-342X (Online). 2017;11(2):768- 776.

Diatta K, Diatta W, Fall AD, Dieng SIMB, Mbaye AI, Kougblenou AJAK, Bassène E. Contribution to the study of leaves: Azadirachta indica A. Juss (Meliaceae): Evaluation of the antioxidant activity. Asian Journal of Research in Botany. 2019;2(4):1-8.
DOI: https://doi.org/10.9734/ejmp/2019/v29i230150

Villaño D, Fernàndez-Pachón MS, Moya ML, Troncoso AM, Garcìaparrilla MC. Radical scavenging ability of polyphenolic compounds
towards DPPH free radical. Talanta. 2007;71:230-235.
DOI: 10.1016/j.talanta.2006.03.050

Shalom Nwodo Chinedu, Okwuchukwu K. Eboji, Solomon O. Rotimi. Effects of Solanum aethiopicum fruit on plasma lipid profile in rats. Advances in Bioresearch. 2013;4(4):79-84.