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Aims: This study aimed to determine the effective percentage of telang leaf meal on feed to increase antioxidant levels in tilapia liver feed.
Study Design: A total of 12 aquariums with sizes 60 x 40 x 40 cm3and volume 96 L prepared and filled with 30 L water. Heater was installed and the temperature was raised slowly until it reached 34℃. Feed in the form of a mixture of telang leaf flour and commercial feed was given 3 times a day according to treatment and with the provisions of 3% of body weight of fish per day. Analysis of antioxidant levels was analyzed from the content of the superoxide dismutase enzyme as an antioxidant bioindicator in the body of the fish.
Place and Duration of Study: The cultivation was carried out at Ciparanje Hatchery and Fisheries Area, Faculty of Fisheries and Marine Science, Universitas Padjadjaran and Dismutase Superoxide Level Analysis was carried out at the Central Laboratory of Universitas Padjadjaran in June and July 2019.
Methodology: This research was carried out by giving high temperatures as a source of stress, for 40 days. Tilapia were given a mixture of telang leaf meal as a source of anti-oxidants. At the end of research, Test liver fish taken superoxide dismutase levels were observed and analyzed descriptively. Daily growth and survival rate of fish were analysed using F test at 5% level, and if there were significant differences, Duncan's test was performed.
Results: By giving heat stress continuously for 40 days the result is obtained the highest average superoxide dismutase level in treatment B (telang leaf meal 5%) as much -0.92 inhibition and lowest in treatment D (telang leaf meal 15%). A decrease in SOD levels identifies a decrease in antioxidant activity in the body of Tilapia. Daily growth rate obtained showed no significant difference. The fish will continue to grow but not as rapidly, because tilapia utilize protein up to 40% of commercial feed used to repair cells damaged during stressful periods and as a source of energy. Survival of fish decreases with increasing concentration of telang leaf flour, where the feed given has been mixed and resulted in the fish's response to the feed decreases, consequently not only the fish adapted to the environment, fish also adapt to new types of feed.
Conclusion: Based on the results of research that has been done then conclusions can be drawn that the addition of telang leaf mealup to 15% in commercial feed by giving heat stress since the beginning of the culture period does not provide an effect of increasing antioxidant levels.
Ath-Thar MHF, Gustiano R. Best Tilapia performance in media salinity. Proceedings of the Aquaculture Technology Innovation Forum. 2010;493-499.
FAO, Food and Agriculture Organization of the United Nations 2019. Fisheries and Aquaculture Statistics (Rome: Food and Agriculture Organization of the United Nations); 2017.
Marine and Fisheries Ministry. Statistics Book 2012 Maritime Affairs and Fisheries: Jakarta; 2013.
Kementerian Kelautan dan Perikanan. ne Data on Marine Production and Fisheries in 2017. Jakarta; 2018.
Angreni NPW, Arthana IW, Wulandari E. Distribution of pathogenic bacteria in Tilapia (Oreochromis niloticus) on Lake Batur Bali. Current Trends in Aquatic Sccience. 2018;96-103.
Payung CN, Manoppo H. Increased non-specific immune response and tilapia growth (Oreochromis niloticus) through giving ginger (Zingiber officinale). Journal of Aquaculture. 2015;3(1):11-18.
Rana KJ, Siriwardena S, Hasan MR. Impact of rising feed ingredients prices on aquafeeds and aquaculture production. FAO Fisheries and Aquaculture Technical Paper. 2009;63.
Magdalena S, Natadiputri GH, Nailufar F, Purwadaria T. Utilization of natural materials as functional feed. Wartazoa. 2013;23(1).
Panagan AT. Effect of addition of carrot flour (Daucus carota L.) against peroxide numbers and free fatty acids in bulk cooking oil. Journal of Science Research; 2011.
Sushma NJ, Prathyusha D, Swathi G, Madhavi T, Raju BDP, Mallikarjuna K. Facile approach to synthesize magnesium oxide nanoparticles by using Clitoria ternatea-characterization and in vitro antioxidant studies. Appl. Nanosci. 2015;6: 437–444.
Vankar PS, Srivastava J. Evaluation of anthocyanin content in red and blue flowers. International Journal of Food Engineering. 2010;6(4).
Lakshmi CHNDM, Raju BDP, Mahdavi T, Sushma NJ. Identification of bioactive compounds by FTIR analysis and in vitro antioxidant activitiy of Clitoria ternatea leaf and flower extracts. Indo American Journal of Pharmaceutical Research. 2014;4(9).
Marklund, Marklund G. Involvement of the superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-474.
Campanella L, Bonanni A, Finotti E, Tomassetti M. Biosensors for determination of total and natural antioxidant capacity of red and white wines: Comparison with other spectrophotometric and fluorimetric methods. Biosensors and Bielectronics. 2004;19:641–651.
Effendie MI. Biological methods of fisheries. Faculty of Fisheries. Institut Pertanian Bogor; 1997.
Djajasewaka HY. Fish feed. Penebar Swadaya. Jakarta; 1985.
Mir MI, Khan S, Bhat SA, Reshi AA, Shah FA, Balki MH, Manzoor R. Scenario of genotoxicity in fishes and its impact on fish industry. Journal of Environmental Science, Toxicology and Food Technology. 2014;8(6):65-76.
Sreejai Rand Jaya DS. Studies on the changes in lipid peroxidation and antioxidants in fishes exposed to hydrogen sulfide. Toxicol Int. 2010;17:7177.
Kumar RT, Kumar S, Anju VS. Phytochemical and antibacterial activities of crude leaf and extracts of Clitoria ternatea varieties (Fabaceae). Journal of Pharmacognosy and Phytochemistry. 2017;6(6):1104-1108.
Sutari VT, Sugito, Aliza D, Asmarida. Malondialdehyde levels (MDA) in the tilapia liver (Oreochromis niloticus) who were given hot stress and jaloh flour supplementation feed. Jurnal Medika Veterinaria. 2013;7(1).
Yunita EA, Nanik HS, Jafron WH. Effect of Teklan leaf extract (Eupatorium riparium) against mortality and growth of larvae Aedes aegypti. BIOMA. 2009;11(1).
Elabd H, Soror E, El-Asely A, El-Gawad EA, Abbass A. Dietary supplementation of Moringa leaf meal for Nile tilapia Oreochromis niloticus: Effect on growth and stress indices. Egyptian Journal of Aquatic Research. 2019;45(3):1-7.
Nithianantham K, Shyamala M, Chen Y, Latha LY, Jothy SL, Sasidharan S. Hepatoprotective potential of Clitoria ternatea leaf extract against paracetamol induced damage in mice. Molecules. 2011;16(12):10134-10145.
Zhang J, Jiang F, Yang P, Li J, Yan Gand Hu L. Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria. Acta Physiol. Plant. 2015;37(7).
Mulyani YS, Yulismanand Fitrani M. Growth and efficiency of tilapia feed (Oreochromis niloticus) periodically fasted. Jurnal Akuakultur Rawa Indonesia. 2014;2(1):1-12.
Pandit NP, Nakamura M. Effect of high temperature on survival, growth and feed conversion ratio of Nile tilapia (Oreochromis niloticus). Out Nature. 2010;8:219-224.
Yanti Z, Muchlisin ZA, Sugito. Tilapia fish growth and survival (Oreochromis niloticus) in several jaloh flour concentrations (Salix tetrasperma) in the feed. Journal of Fisheries and Aquatic Sciences. 2013;2(1):16-19.
Drummond CD, Murgas LDS, Vicentini B. Growth and survival of tilapia (Oreochromis niloticus) submitted to different temperatures during the process of sex reversal. Cienc. Agrotec. 2009;33(3):895-902.
Leal E, Fernandez B, Guillot R, Riot D, Miguel J, Reverter C. Stress induced effects on feeding behavior and growth performance of the Sea Bass (Dicentrarchus labrax): A self feeding approach. J Comp Physiol B. 2011;181(8): 1035-44.
Aliza D, Winaruddin, Sipahutar LW. Effects of increased water temperature on behavior, anatomical pathology, and tilapia gills pathology (Oreochromis niloticus). Jurnal Medika Veterinaria. 2013;853- 1943.
Nugroho A. Water quality bioindicator. Penerbit Universitas Trisakti, Jakarta; 2006.
Odum EP. Fundamental of ecology. W. B. Saunder Com. Philadelphia; 1971.
Effendi H. Water quality study. Kanisius. Yogyakarta; 2003.