Evaluation of Stress Enzymes Activities and Lipid Peroxidation in Heart Homogenates of Male Albino Rats Following the Administration of Diclofenac

Main Article Content

Felicia Nmeazi Okwakpam
S. Abarikwu
M. O. Monanu

Abstract

Nonsteroidal anti-inflammatory drugs are associated with an increase in cardiovascular events despite its uses in the therapeutic agent for the management of long- and short-term pain. Over the last years, evidence has accumulated showing that oxidative stress plays an important role in the pathogenesis of cardiovascular diseases. Oxidative stress is no longer considered as a simple imbalance between the production and scavenging of reactive oxygen species (ROS), but as a dysfunction of enzymes involved in ROS production. This study investigated the effect of diclofenac on the activity of oxidative stress enzymes as well as formation of lipid peroxidation. Male rats weighing about 100-120 g were divided into four groups: group one (control, feed+water) group two, group three and group four treated with different mg/kg/day of drugs (50 mg/kg/day, 100 mg/kg/day and 150 mg/kg/day) feed and water respectively for 7 days. Analysis on the effect of diclofenac on the activities of stress enzymes such as nicotine adenosine dinucleotide phosphate hydrogenase oxidase (NADPHoxidase), xanthine oxidase(XOD), catalase(CAT), superoxide dismutase(SOD) and Glutathione Peroxidase as well as evaluation of lipid peroxidation by measuring malondialdehyde (MDA) in the heart homogenate were carried out and the result showed a significant increase in each parameter given rise to the production of reactive oxygen species (ROS) if not moderated by the antioxidant defense can lead to cardiac impairment as a result of oxidative stress damage or injury. The result obtained implies that diclofenac (NSAIDs) affects the redox status of vascular tissues (heart tissues).

Keywords:
Nonsteroidal anti-inflammatory drugs, diclofenac, cardiovascular events, oxidative stress, lipid peroxidation, stress enzymes, heart homogenate

Article Details

How to Cite
Okwakpam, F. N., Abarikwu, S., & Monanu, M. O. (2020). Evaluation of Stress Enzymes Activities and Lipid Peroxidation in Heart Homogenates of Male Albino Rats Following the Administration of Diclofenac. Asian Journal of Research in Biochemistry, 6(3), 10-16. https://doi.org/10.9734/ajrb/2020/v6i330117
Section
Original Research Article

References

Antman EM, DeMets D, Loscalzo J. Cyclooxygenase inhibition and cardio-vascular risk. Circulation. 2005;112(5):759-770.

Hristova M, Penev M. Oxidative stress and cardiovascular diseases. Trakia Journal of Sciences. 2014;3:296-303.

Forstermann U, Munzel T. Endothelial nitric oxide synthase in vascular disease: from marvel to menace. Circulation. 2006;113 (13):1708-1714.

Abdulrauf RA, Dawud FA, Emmanuel NS, Muhammad HD. Lipid peroxidation and some antioxidant enzymes evaluation in Apple Cider Vinegar (ACV) treated male and female wistar rats exposed to chronic restraint stress. Advances in Enzyme Research. 2018;6:21-28.

Obediah GA, Paago G. Effects of ethanolic extract of Moringa oleifera seeds and leaves on pregnancy. Sch. Int. J. Biochem. 2018;1(3):101-105.

Obediah GA, Paago G. Effects of ethanolic extract of M. oleifera seeds and leaves on the reproductive system of female albino rats. SOJ Biochem. 2018;4(1):1-8.
Available:http://dx.doi.org/10.15226/2376-4589/4/1/0013

Deavall DG, Martin EA, Horner JM, Roberts R. Drug-induced oxidative stress and toxicity. Journal of Toxicology. 2012;1-13.

Gladden JD, Zelickson BR, Guichard JL. Xanthine oxidase inhibition preserves left ventricular systolic but not diastolic function in cardiac volume overload. The American Journal of Physiology Heart and Circulatory Physiology. 2013;305(10):1440-1450.

Okwakpam FN, Omeodu SI, Uwakwe AA. Effects of diazepam on selected blood enzymes activity and prostrate specific antigen of adult male wister rats. American Journal of Biomedical Sciences. 2018;10 (3):157 – 168.

EL-Awady MS, Goda EA, Laila A. Eissa1. NADPH oxidase inhibition protects against doxorubicin-induced cardiotoxicity and inflammation in rats. International Journal of Pharmaceutical Research and Bio-Science. 2014;3(1):459- 470.

Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976; 72:248-254.
Available:http://dx.doi.org/10.1016/0003-2697(76)90527-3

Taha NR, Rabah SO, Shaker SA, Mograby MM. Effect of Moringa oleifera leaves on diclofenac sodium induced hepatic injury in albino rats: Ultrastructural and Immunohistochemical Studies. Journal of Cytology and Histology. 2015;6(2):137-154.

Sahoo S, Meijles DN, Pagano PJ. NADPH oxidase: Key modulators in aging and age-related cardiovascular diseases. Clinical Science. 2016;130(5):317-335.

Huige L, Marcus H, Andreas D, Mattias O, Mir AO. Cyclooxygenase 2-selective and non-selective non-steroidal anti-inflammatory drugs induce oxidative stress by up-regulating vascular NADPH oxidase. Journal of Pharmacology and Experimental Therapeutic. 2008;326:745-758.

Li H, Hortmann M, Daiber A, Oelze M, Ostad MA, Schwarz PM, Xu H, Xia N, Kleschyov AL, Mang C, Warnholtz A, Münzel T, Förstermann U. Cyclooxyge-nase 2-selective and nonselective nonsteroidal anti- inflammatory drugs induce oxidative stress by up- regulating vascular NADPH oxidases. J. Pharmacol. Exp. Ther. 2008;326(3):745-53.

Zhao Y, McLaughlin D, Robinson E, Harvey AP, Hookham MB, Shah AM, McDermott BJ, Grieve DJ. Nox2 NADPH oxidase promotes pathologic cardiac re- modeling associated with Doxorubicin chemotherapy. Cancer Res. 2010;70(22):9287-9289.

Chelikani P, Fital Loewen PC. Diversity of structures and properties among catalase. Cellular and Molecular Life Sciences. 2004; 61(2):192-208.

Ahmad MS, Yousaf M, Mothana RA, Al-Rehaily AJ. Evaluation of acute toxicity and anti-inflammatory effects of Baccharoides schimperi (DC.) In Experimental Animals. Afr J Tradit Complement Altern Med. 2016;12(1):99-103.