Asian Journal of Research in Biochemistry 2020-05-24T05:12:22+00:00 Asian Journal of Research in Biochemistry Open Journal Systems <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Evaluation of the Molluscicidal, Artemicidal and Cytotoxic Activities of the Lectin from Opuntia ficus-indica Cladodes (OfiL) 2020-05-24T05:12:22+00:00 Giselly Maria de Sá Santana Lidiane Pereira de Albuquerque Emmanuel Viana Pontual Luanna Ribeiro Santos Silva Jaciana dos Santos Aguiar Ana Maria Mendonça de Albuquerque Melo Thiago Henrique Napoleão Patrícia Maria Guedes Paiva <p><strong>Background: </strong><em>Biomphalaria glabrata</em> is an intermediate host for the larvae of <em>Schistosoma mansoni</em>, which is distributed widely in the tropics. <em>B. glabrata </em>control is important to minimize the spread of schistosomiasis and natural compounds have been sought for use against this disease. The <em>Artemia salina </em>bench-top bioassay has been used to investigate the ecotoxicity of many natural compounds, and its results also correlate well with the <em>in vitro</em> cytotoxicity of natural compounds to tumor cells.</p> <p><strong>Aims: </strong>To evaluate deleterious effects of the <em>Opuntia ficus-indica </em>lectin (OfiL) on <em>B. glabrata</em>,&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <em>&nbsp;A. salina </em>and human cancer cell lines.</p> <p><strong>Methods: </strong>OfiL was isolated following a previously established protocol. The effects of OfiL on &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<em>B. glabrata </em>were investigated by determining survival of adults as well as development and hatching of embryos. The concentration required to kill 50% (LC<sub>50</sub>) of <em>A. salina </em>nauplii was determined. The cytotoxicity was determined using the human cell lines Hep-2 (human larynx epidermoid carcinoma), NCI-H292 (human lung mucoepidermoid carcinoma) and K562 (chronic myelocytic leukemia).</p> <p><strong>Results: </strong>The development of most embryos (92.5–97.5%) treated with 1, 10 and 100 µg/mL of OfiL was found to be delayed, and dead (2.2–3.3%) and malformed (0.3–5.2%) embryos were also observed. OfiL did not kill <em>B. glabrata </em>adults, but a high percentage (30–45%) of the embryos generated by snails incubated with the lectin exhibited malformations. OfiL exhibited toxicity against <em>A. salina </em>(LC<sub>50</sub>: 61.02 µg/mL) but did not display cytotoxicity against the tumor cell lines evaluated.</p> <p><strong>Conclusion: </strong>In conclusion, this study showed that OfiL can be a tool for schistosomiasis control that acts by impairing the viability of <em>B. glabrata </em>eggs and the fecundity of adult snails.</p> 2020-05-18T00:00:00+00:00 ##submission.copyrightStatement## Evaluation of Stress Enzymes Activities and Lipid Peroxidation in Heart Homogenates of Male Albino Rats Following the Administration of Diclofenac 2020-05-24T05:12:21+00:00 Felicia Nmeazi Okwakpam S. Abarikwu M. O. Monanu <p>Nonsteroidal anti-inflammatory drugs are associated with an increase in cardiovascular events despite its uses in the therapeutic agent for the management of long- and short-term pain. Over the last years, evidence has accumulated showing that oxidative stress plays an important role in the pathogenesis of cardiovascular diseases. Oxidative stress is no longer considered as a simple imbalance between the production and scavenging of reactive oxygen species (ROS), but as a dysfunction of enzymes involved in ROS production. This study investigated the effect of diclofenac on the activity of oxidative stress enzymes as well as formation of lipid peroxidation. Male rats weighing about 100-120 g were divided into four groups: group one (control, feed+water) group two, group three and group four treated with different mg/kg/day of drugs (50 mg/kg/day, 100 mg/kg/day and 150 mg/kg/day) feed and water respectively for 7 days. Analysis on the effect of diclofenac on the activities of stress enzymes such as nicotine adenosine dinucleotide phosphate hydrogenase oxidase (NADPHoxidase), xanthine oxidase(XOD), catalase(CAT), superoxide dismutase(SOD) and Glutathione Peroxidase as well as evaluation of lipid peroxidation by measuring malondialdehyde (MDA) in the heart homogenate were carried out and the result showed a significant increase in each parameter given rise to the production of reactive oxygen species (ROS) if not moderated by the antioxidant defense can lead to cardiac impairment as a result of oxidative stress damage or injury. The result obtained implies that diclofenac (NSAIDs) affects the redox status of vascular tissues (heart tissues).</p> 2020-05-18T00:00:00+00:00 ##submission.copyrightStatement##