http://journalajrb.com/index.php/AJRB/issue/feed Asian Journal of Research in Biochemistry 2020-01-20T15:38:12+00:00 Asian Journal of Research in Biochemistry contact@journalajrb.com Open Journal Systems <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> http://journalajrb.com/index.php/AJRB/article/view/30106 Hematotoxic Effect of Water Soluble Fraction of Bonny Light Crude Oil in Wistar Albino Rats 2020-01-20T15:38:12+00:00 Abraham, Chiedozie Nicholas chiedozie_abraham@uniport.edu.ng Godwin J. Udom Kingsley C. Patrick-Iwuanyanwu <p>This study evaluated the Hematotoxic effect of water soluble fraction (WSF) of Bonny Light Crude Oil (BLCO) in Wistar Albino rats. After preparation of the WSF and a range finding test, the Wistar albino rats were administered three concentrations (25%, 50% and 100%) of WSF of BLCO for 30 and 60days. Data from the study showed that there was a significant decrease (p≤0.05) in PCV values (30.2% in the control group to 17.2% in the 100% group), Hb levels decreased significantly from 9.25 g/dl in the control group to 5.27 g/dl in the 100% group. WBC count decreased significantly from 2932 mm<sup>3 </sup>in the control group to 136 mm<sup>3</sup> in the 100% group and finally RBC count decreased significantly from 241.8E4 mm<sup>3</sup> to 567 mm<sup>3</sup> with increasing concentrations in the treatment groups after 60days administration. These results suggest that the oral consumption of the WSF of BLCO led to an onset of anaemia which indicates the presence of less than normal concentrations of PCV, Hb and RBC.</p> 2020-01-10T00:00:00+00:00 ##submission.copyrightStatement## http://journalajrb.com/index.php/AJRB/article/view/30107 Evaluation of the Antioxidant Activity of Stalk and Fruit of Solanum aethiopicum L. (Solanaceae) 2020-01-20T15:38:11+00:00 Kady Diatta khadydiose@yahoo.fr William Diatta Alioune Dior Fall Serigne Ibra Mbacké Dieng Amadou Ibrahima Mbaye Abdou Sarr Mourchidath Adjoke Adegbindin <p><strong>Background: </strong>The use of plants for healing dates back to very remote times. Nowadays with the accession of new diseases plants are increasingly used for the formulation of new drugs able to overcome the many diseases (cancer, atherosclerosis) often caused by the disorder of the system prooxidant/antioxidant.</p> <p><strong>Aim/Objective:</strong> On the strength of this observation, the research of an antioxidant plant is essential, hence the aim of this study, which is to determine the antioxidant activity of the stalk and the fruit of <em>Solanum aethiopicum</em> L.</p> <p><strong>Methods:</strong> The fruits and stalk were washed, cut into fine slats, then dried in the incubator for three days and finally crushed into powder. An extraction by decoction with ethanol (stalks and fruits) and water (fruit) was subsequently carried out to obtain three extracts (ethanol and water). Antioxidant activity was evaluated through the FRAP method, and the trapping of radical DPPH.</p> <p><strong>Results:</strong> For the FRAP method, at the highest concentration (1 mg/ml) the aqueous extract of the fruit (74.84±2.97%) has a higher reducing power compared to those of the ethanolic extracts of the fruit (70.15 ± 5.72%) and the stalk (49.85 ± 2.11%). These reducing powers, although significant, remain lower than those of tannic acid (89.95±0.007%). And finally, for the DPPH method, the aqueous extract of the fruit is more effective in reducing free radical DPPH with a IC<sub>50</sub>= 162±33 µg/ml, follow up by ethanol extract from the stalk (IC<sub>50</sub>= 360± 90 µg/ml) and finally ethanol extract from the fruit (IC<sub>50</sub>= 362.5± 23.5 µg/ml). These results confirm the <em>in vitro</em> antioxidant activity of the studied parts of <em>Solanum aethiopicum.</em></p> <p><strong>Conclusion: </strong>Prospective studies could focus on acute and subacute toxicities and the determination of the molecules responsible for the activity.</p> 2020-01-11T00:00:00+00:00 ##submission.copyrightStatement##