Asian Journal of Research in Biochemistry <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Asian Journal of Research in Biochemistry en-US Asian Journal of Research in Biochemistry 2582-0516 Comparative Study on Effect of Chrysophyllum albidum Medicinal Plant from Crude Oil Polluted and Non-crude Oil Polluted Areas on Selected Biochemical Parameters in Rats <p>Comparative study on effect of <em>Chrysophyllum albidum</em> medicinal plant from crude oil polluted and non-crude oil polluted areas on selected biochemical parameters in rats was evaluated.&nbsp; Leaves of <em>C. albidium </em>were collected from a botanical garden (non-crude oil polluted site) and Okrika (crude oil polluted site), and studied. The leaves passed for heavy metals analyses and heavy metals such as mercury (0.38 ±0.07 mg/100 g), lead (3.06±0.40 mg/100 g), cadmium (0.09±0.00 mg/100 g), copper (1.00±0.18 mg/100 g), chromium (0.23±0.01 mg/100 g), and cobalt (4.90±1.22 mg/100 g) were observed in leaves of <em>C. albidum</em>&nbsp; from crude oil polluted area. Rats placed on compounded feed of <em>C. albidium </em>leaves from crude oil polluted area revealed marked degeneration in haematological indices, liver enzymes, urea and creatinine of the kidney. These observed degeneration could be linked to toxicity of the heavy metals found in the leaves of <em>C. albidum</em> from crude oil polluted area since leaves of <em>C. albidum</em> are known to have medicinal potency that could protect the integrity of internal organs and tissues. There is need to properly inform those who rely on medicinal plants from crude oil polluted areas of the possible dangers such may pose in the body. This study has evaluated the comparative effect of <em>C</em><em>.</em><em> albidum </em>medicinal plant from crude oil polluted and non-crude oil polluted areas on selected biochemical parameters in rats.</p> Grace Ekpo Benjamin Amadi Eze Adindu Odey Michael Princewill Dasimeokuna ##submission.copyrightStatement## 2019-11-15 2019-11-15 1 8 10.9734/ajrb/2019/v5i430096 Antiplasmodial, Hepatic and Nephritic Effects of Fractions of Methanol Leaf Extract of Glyphaea brevis in Plasmodium berghei-Infected Mice <p>This study evaluated the antiplasmodial, hepatic and nephritic effects of fractions of<em> Glyphaea brevis</em> methanol leaf extract in <em>P. berghei </em>infected mice. Mice weighing between 15-30 g were infested intraperitoneally with 0.2ml plasmodium infected blood and left for 3 hours before treatment. Infected test groups were treated via oral route of administration with varying doses (200, 300 and 400 mg/kg body weight) of ethylacetate, <em>N-</em>butanol and residual aqueous portion fractions of the <em>Glyphaea brevis</em> methanol extract and Artemisinin (5 mg/kg b.wt) for four days. <em>N-</em>butanol fraction showed the highest antiplasmodial activity (76.64%), followed by residual aqueous portion (73.25%) and ethylacetate (72.99%); Artemisinin has 86.13%. Serum bilirubin (total and conjugated) concentrations of the untreated group (0.82 ± 0.20, 0.51 ± 0.12) were significantly lower (<em>P</em>&lt;0.05) than those in the infected group treated with 300 mg/kg of the residual aqueous portion (1.36 ± 0.20, 0.76 ± 0.05) respectively. Serum albumin levels showed significant (<em>P</em>&lt;0.05) increase in all the groups treated compared to the positive control. Serum total protein, urea and creatinine levels of test groups were not significantly (<em>P</em>&gt;0.05) different from the positive control group. Conclusively, <em>Glyphaea brevis </em>has substantial antiplasmodial activity and could provide a lead for new antimalarial drug development.</p> Tayo Micheal Anjuwon Oluwafemi Abiodun Adepoju Olufemi Adewale Adeniran ##submission.copyrightStatement## 2019-10-28 2019-10-28 1 8 10.9734/ajrb/2019/v5i430094 The Diagnostic Value of the Glucose Curve in Individuals with and without Diabetes <p><strong>Aim:</strong> Of this study is to demonstrate the importance of glucose curve test in monitoring pre and post-meal variation in diabetic and normal individuals.</p> <p><strong>Methodology:</strong> The individuals subjected to this study mainly grouped in two categories the (DM2 group) and the (Control group), they instructed to came fasting at which blood sample will be collected in EDTA and blank tube then after 30 min. the first post-prandial blood sample collected and then after every 1,2,3,4,5,6,7,8 hours blood sample collected subsequently, then serum separated from each sample (except the EDTA tube) analysed biochemically for glucose and glycated haemoglobin HbA1c (from EDTA tube).</p> <p><strong>Result:</strong> We found that, the calculated glucose based on mean glycated haemoglobin HbA1c% results underestimate the real concentrations all over the glucose curve in control group but in DM2 group it underestimate the mean and some actually measured concentration in some points of the curve which adds more burden on the diabetic patient and the responsibility of adjusting the dose and time of administration.</p> <p><strong>Conclusion: </strong>from our prospect we recommend the use of blood glucose curve as a monitoring and diagnostic tool generally for glucose metabolism in normal, pre-diabetic, diabetic and uncontrolled diabetic patients before and during therapeutic conditions.</p> Mohamed S. El-Gareb Mohamed N. El-Naggar ##submission.copyrightStatement## 2019-10-28 2019-10-28 1 7 10.9734/ajrb/2019/v5i430095 The Organ Distribution, Characterization and Modification of Acetylcholinesterase Activity in Adult African Grasshopper: Zonocerus sp Linn. <p><strong>Aim</strong><strong>: </strong>To determine the organ distribution and characterization of acetylcholinesterase in the adult African variegated grasshoppers – <em>Zonocerus variegatus</em> and <em>Zonocerus elegans</em>. (<em>Zonocerus</em> Sp. <em>Linn</em>)</p> <p><strong>Place and Duration of the Study</strong><strong>:</strong> The insect model: African variegated grasshoppers are gotten from the Open green fields at the Federal University of Technology, Akure, Nigeria, and research was carried out between March and June, 2016 in the Enzymology laboratory, Biochemistry department, Federal University of Technology, Akure, Nigeria.</p> <p><strong>Methodology: </strong>Twenty (20) adults variegated grasshoppers were taken from the Open field in the University community, and taken to the Biology department for Identification. After identification, the specimen was weighed, freeze, dissected into fractions (Head, Thorax and Abdomen) and then homogenized to get the crude protein extract. The crude enzyme extract is further purified using the Ion-exchange chromatography with column bed packed with DEAE – Sephadex A<sub>50</sub>. The protein content of the purified AChE was determined using the Lowry method while the Acetylcholinesterase activity was determined by the Ellman’s assay procedures. The characterization of AChE was tested by modifying agent such as N-Bromo Succinamide (NBS) which confirms the presence of key aromatic proteins involve in catalysis at the active site of the enzyme.</p> <p><strong>Results: </strong>The protein concentration according to their fractions: Head (35.7%), Thorax (29.2%), and Abdomen (35.1%). The AChE activity according to their fractions: Head (38.6%), Thorax (23.7%), and Abdomen (37.7%). The specific activity which relates the AChE activity to protein content is given: Head (28.8%), Thorax (40.4%), and Abdomen (30.8%). From the Organ distribution and AChE activity, it was observed that the Head Fractions has the Highest protein content, and Enzyme activity. Comparatively, there are slight differences in the Enzyme activity of the Head and Abdominal fractions which represents the two peaks in the AChE chart. As well, the thorax has the highest specific activity. The modification by the chemical agent NBS shows a drastic decrease (about 50%) in Enzyme activity and characterize enzyme active site with aromatic proteins especially tryptophan residues.</p> <p><strong>Conclusion: </strong>Research findings shows the dominance of AChE protein in the Head region, hence high enzyme activity (useful for nervous coordination) as well as presence of tryptophan residues at the enzyme active site. The importance of research is useful in enzymology, neuroscience and public health.</p> E. A. Fajemisin O. S. Bamidele S. O. Ogunsola E. A. Aiyenuro ##submission.copyrightStatement## 2019-11-19 2019-11-19 1 9 10.9734/ajrb/2019/v5i430097