Asian Journal of Research in Biochemistry http://journalajrb.com/index.php/AJRB <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US contact@journalajrb.com (Asian Journal of Research in Biochemistry) contact@journalajrb.com (Asian Journal of Research in Biochemistry) Wed, 19 Feb 2020 11:53:47 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Effect of a Combination of Ethanol Extract of Ficus capensis and Cnidoscolus aconitifolius on Liver and Kidney Function Parameters of Phenylhydrazine-Induced Anemic Rats http://journalajrb.com/index.php/AJRB/article/view/30111 <p><strong>Background: </strong>Anemia is a condition in which there is a reduced number of red blood cells or haemoglobin and iron concentration in the body thereby leading to a decreased capacity of the blood to carry oxygen to the body tissues.</p> <p><strong>Objectives:</strong> The study was designed to investigate the effect of a combination of ethanol extract of <em>Ficus capensis</em> and <em>Cnidoscolus aconitifolius</em> in phenylhydrazine-induced anemic rats.</p> <p><strong>Methods:</strong> The animals were divided into five groups of five rats each. Group A served as normal control, Group B as anemic control, group C as standard drug control, groups D and E were treated with 200 mg/kg and 400 mg/kg of combined ethanol leaf extract of <em>F. capensis </em>and <em>C. aconitifolius</em> respectively. Phenylhydrazine was administered intraperitoneally at a dose of 20 mg/kg b.w. for two days to induce anemia in rats. The administration of the extract lasted for 14 days after which the animals were sacrificed and blood obtained through cardiac puncture for kidney and liver biomarkers analyses.</p> <p><strong>Results:</strong> Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, Total bilirubin, Direct bilirubin and Kidney function parameters assayed in the anemic untreated group showed significant increases (<em>p</em>&lt;0.05) compared to the normal rats which may be attributed to toxicity induced by phenylhydrazine. The groups treated with the ethanol extract at a dose of 200 and 400 mg/kg body weight revealed a significant (<em>p</em>&lt;0.05) decrease in the liver function parameters compared to the anemic untreated group. The creatinine and urea levels of the anemic untreated group increased more than the that of the test groups although the increase was not statistically significant.</p> <p><strong>Conclusion:</strong> This study suggests that combined ethanol leaf extract of <em>F. capensis</em> and <em>C. aconitifolius</em> is safe and can be effective in the treatment and management of anemia.</p> Ezeigwe, Obiajulu Christian, Okpala, Chukwuemeka Obumneme, Ogbodo, Uchechukwu Chibuzo, Anwuna, Chioma Divine, Ngwu, Ogochukwu Rita, Chigbo, Chidiebere Malachy ##submission.copyrightStatement## http://journalajrb.com/index.php/AJRB/article/view/30111 Wed, 19 Feb 2020 00:00:00 +0000 Mineral, Fatty and Amino Acids Composition of Three Species of Mollusks (Egeria radiata, Limicolaria flammea and Viviparus contectus) http://journalajrb.com/index.php/AJRB/article/view/30112 <p>The present study investigates the mineral, fatty acid and amino acids composition of <em>E. radiata, &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;V. contectus </em>and <em>L. flammea</em>. All samples analyzed in this study were obtained from Yenagoa in Bayelsa state of Nigeria. Mineral composition was determined by Atomic Absorption Spectrophotometry (AAS), fatty acids were determined by High Performance Liquid Chromatography (HPLC) while amino acids composition was determined by gas chromatography (GC). Eight (8) nutritionally essential minerals were detected in all samples analyzed in this study; the concentration of minerals in order of increasing concentration was Cu&gt;Mn&gt;Mg&gt;Na&gt;Ca&gt;Zn&gt;K&gt;Fe, Cu&gt;Mn&gt;Na&gt;Mg&gt;Ca&gt;Zn&gt;K&gt;Fe and Cu&gt;Mn&gt;Mg&gt;Ca&gt;Na&gt;Zn&gt;Fe&gt;K for <em>E. radiata, V. contectus </em>and<em> L. flammea </em>respectively. Lauric acid, myristic acid, Palmitic acid, Margaric acid, stearic acid, oleic acid, Linoleic acid, arachidic acid and arachidonic acid were detected in varying amounts in all samples. Palmitic acid concentration in <em>E. radiata </em>was the most abundant in all samples while oleic acid concentration in <em>L. flammea </em>was the least. A total of eighteen (18) amino acids were detected in all samples analyzed in this study: glycine, alanine, valine, leucine, isoleucine, serine, tryptophan, threonine, Methionine, phenylalanine, histidine, proline, aspartic acid, glutamic acid, tyrosine, arginine, lysine and Cysteine. In <em>E. radiata, </em>leucine had the highest concentration (21.287mg/100g) while proline was the least (2.854mg/100g); glutamic acid and methionine were the highest and least (19.389mg/100g and 2.996mg/100g respectively) in <em>V. Contectus </em>samples and Histidine and tryptophan were the highest and least respectively (11.639mg/100g and 1.415mg/100g).&nbsp; Aspartate, lysine and histidine were not detected in both samples. From the findings of this study, the samples analyzed in this study are good sources of fatty acids, amino acids and nutritionally essential minerals hence their consumption is encouraged.</p> I. Felagha, M. O. Monanu, B. A. Amadi ##submission.copyrightStatement## http://journalajrb.com/index.php/AJRB/article/view/30112 Thu, 19 Mar 2020 00:00:00 +0000 Effect of the Garcinia kola Seed on Serum Lipids in Adult Subjects http://journalajrb.com/index.php/AJRB/article/view/30113 <p><strong>Background:</strong> Many studies have reported the lipid-lowering effect of the <em>Garcinia kola</em> seed in experimental animals.</p> <p><strong>Objective:</strong> Assessing the effect of daily intake of <em>Garcinia kola</em> seed on serum lipids in adult subjects.</p> <p><strong>Methods:</strong> This research work was an intervention study based on a quasi-experimental approach carried out over a three-month period i.e. from May 1 to July 30, 2019. The study sample consisted of 40 adult subjects (18 men and 22 women) who gave their written informed consent to participate to in the study. After receiving required information, each subject underwent a baseline lipid test consisting of total cholesterol (TC), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C) and triglycerides. Then, each subject consumed every day one <em>Garcinia kola </em>seed during 90 days. Every 30 days, a lipid test was performed. TC, HDL-C and triglycerides were determined using end-point colometric enzyme assay<strong>.</strong> LDL-C was estimated using the formula of Friedewald. Student’s t test helped compare the mean values of lipid parameters at the beginning and at the end of the experiment at the threshold of 5%.</p> <p><strong>Results:</strong> At the beginning and at the end of the experiment, the mean values in g/L of TC (1.68±0.35 vs 1.60±0.31), HDL-C (0.53±0.13 vs 0.51±0.14), LDL-C (0.97±0.36 vs 0.94±0.34) and triglycerides (0.91±0.50 vs 0.72±0.33) were not significantly different (p &gt; 0.05). However, we noted a downward trend in those values over time during the experiment.</p> <p><strong>Conclusion:</strong> The daily consumption of <em>Garcinia kola</em> seed improves serum lipid profile. The use of that seed is a potential alternative to the conventional treatment of dyslipidemia.</p> Moutawakilou Gomina, Tarik Salifou, Gilbert Djidonou, Stanislas Zinsou ##submission.copyrightStatement## http://journalajrb.com/index.php/AJRB/article/view/30113 Thu, 02 Apr 2020 00:00:00 +0000