Physicochemical Characterisation and Determination of the Effect of Temperature on the Amylase Activities of Germinated Sorghum and Maize Grains
Fagbohoun Jean Bedel *
Department of Biochemistry-Genetics, Peleforo Gon Coulibaly University (UPGC), Korhogo, BP-1328 Korhogo, Côte d’Ivoire.
Anon Attoh Hyacinthe
Laboratory of Biochemistry, Microbiology and Valorization of Agro-Resources (LBMVA), Institute of Agropastoral Management, Peleforo Gon Coulibaly University (UPGC), Korhogo, BP-1328, Korhogo, Côte d’Ivoire.
Coulibaly Seydou Ouolouho
Department of Biochemistry-Genetics, Peleforo Gon Coulibaly University (UPGC), Korhogo, BP-1328 Korhogo, Côte d’Ivoire.
Kouamé Lucien Patrice
Department of Food Science and Technology, University Nangui Abrogoua, Abidjan, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Maize and sorghum are staple foods in many African countries. Along with rice, they constitute the bulk of the cereals consumed by the populations of northern Côte d'Ivoire. These cereals are used either directly or indirectly in malted and/or fermented form in the preparation of several traditional foods and the production of alcoholic beverages such as dolo a local beverage. This study aims at the physicochemical characterization and the determination of the effect of temperature on the amylase activities of germinated cereal grains of corn and sorghum collected on the market of the city of Korhogo. To do this, the analysis of cereals germinated over 6 days revealed that amylase activities are optimal on the second day of germination with a diastatic power of 110.29 ± 1.8 IU / g of protein and 114.17 ± 2, 4 IU/g of protein respectively for sorghum and maize. The optimal temperatures are mesophilic and are between 40 and 50°C. Beyond these temperatures, amylase activities are denatured until they reach 20% relative activity after exposure to 65°C. During germinationsand at an acid pH, the levels of reducing sugars, polyphenols and tannins increased with the duration of germination. The results of this study revealed that certain physicochemical characteristics and the effect of temperature condition the obtaining of a malt likely to influence the quality of a traditional beer. The germination time set at 2 days makes it possible to obtain fermentable sugars during malting for the preparation of alcoholic beverages according to the production conditions of traditional vendors. For sugars, they increase from 0.39±0.035 mg/100g DM (Dry Matter) before germination to 0.86± 0.003 mg/100g DM on the 6th day of germination for sorghum. As for maize, these levels increase from 0.11± 0.014 mg/100g DM to 0.43± 0.02 mg/100g DM. The diastatic power is optimal on the 2nd day, 110.29 ± 1.8 IU/g of protein for sorghum and 114.17 ± 2.4 IU/g of protein for maize.
Keywords: Germination, amylase activity, maize, sorghum, malting
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Godon B. Les constituants des céréales : nature, propriétés et teneurs. In: Godon B. (Ed.), Biotransformation des produits céréaliers. Ed. Lavoisier Tec & Doc, Paris. 1991 :1-22.
Cissé OIK, Faye G, Ali MS, Ayessou NC, Cissé M, Diatta M. Diagnostic du procédé et caractérisation physicochimique et biochimique d’une boisson fermentée à base de mil: le Boumkaye. Afrique Science. 2016;12: 59-65.
Konzou T, Agbodan KA, Tchani GW, Agbodan KML, Boyode P. Enhancement of local sorghum beer by using a simple chemical quality control method (Densimetry). International Journal of Innovation and Applied Studies. 2020;29(3):628-637.
Shelke GV, Chavan AM. Improvement of agronomicaly desirable genotypes for downy mildew disease resistance in Pearl millet [Pennisetum glaucum (L.) R. Br.] By recombination breeding. Journal of Ecobiotechnology. 2010;2(1):16-20.
Kadri A, Halilou H, Karimou I. Cultivation of millet (Pennisetum glaucum (L) R. Br) and its production constraints: a review. International Journal of Biological and Chemical Sciences. 2019;13(1):503-524.
Dicko MH, Gruppen H, Traoré AS, Voragen AGJ, Van Berkel WJH. (Sorghum grain as human food in Africa: relevance of content of starch and amylase activities. African Journal of Biotechnology. 2006;5:384-395.
Aka S, Djè KM, Fokou G, Doumbia M, Ahoussi JMS, Bonfoh B. Production Et Consommation Du Tchapalo À Abidjan: Typologie Des Brasseuses Et Des Consommateurs. European Scientific Journal. 2017;13(1):148-163.
Chavan JK, Kadam SS. Nutritional improvement of cereals by fermentation. Critical Reviews in Food Science and Nutrition. 1989;28:349-400.
Mbithi-Mwikya S, Van Camp J, Yiru Y, Huyghebaert A. Nutrient and antinutrient changes in finger millet (Eleusine Coracan) during sprouting. Lebensmittel-Wissenschaft und-Technologie. 2000;33: 9-14.
Mbofung CM, Fombang EN. Improving the digestibility and availability of nutrients from sorghum flour through improved malting techniques. 2nd International workshop: Food-based approaches for a healthy nutrition. Ouagadougou. 2003: 489-502.
Kayodé APP, Ahouanse IS, Kotchoni SO, and Hounhouigan JD. Optimisation du procédé traditionnel de maltage du sorgho pour la production de boissons fermentées. International Journal of Biological and Chemical Sciences. 2011; 5(4):1552-1561.
Dewar J, Taylor JRN, Berjak P. Determination of improved steeping conditions for sorghum malting. Journal of Cereal Science. 1997;26: 129-36.
Touwang C, Nso EJ, and Ndjouenkeu R. Production Du Bili-Bili, Biere Traditionnelle De Sorgho Du Nord Cameroun: Diversité des procédés de production et qualité des produits. European Scientific Journal. 2018;14(36):207-224.
Bernfeld P. Amylase α and β, Methods in enzymology 1.S. P, Colswick and N.O.K; 1955.
Giamarchi P. Dosage de l'activité alpha et béta-amylasique. In Methodes d'Analyse, Herzele P (ed). Laboratoire de Nutrition : Brazaville-Congo. 1992;1-8.
AOAC. Official Methods of Analysis (vol 2, 15th ed). Washington DC: Association of Official Analytical Chemists; 1990.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. 1999;299:152-178.
Bainbridge Z, Tomlins K, Willings K, and Westby A. Methods for assessing quality characteristics of non-grain starch staple. Part 4 advanced methods. National resources institute, University of Greenwich, UK ISBN 0-85954-400-. 1996; 43-79.
Pale DP. Kabore SJB, Taonda B, Bougouma SC. Mason. Economic assessment of malt and traditional beer (dolo) production in Burkina Faso S. International Journal of Agricultural Economics and Extension. 2019;7(2):001-006.
Gernah DI, Ariahu CC. and Ingbian EK. Effects of malting and lactic fermentation of some chemical and functional properties of maize (Zea mays L.). American Journal Food Technology. 2011;6:404-412.
[Ayernor GS, Ocloo FCK. Physicochemical changes and diastatic activity associated with germinating paddy rice. Africa Journal of Food Science. 2007;1:37-41.
Chavan JK, Kadam SS. Nutritional improvement of cereals by fermentation. Critical Reviews in Food Science and Nutrition. 1989;28:349-400.
Booysen C, Dicks LMT, Meijering I and Ackermann A. Isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars. International Journal of Food Microbiology. 2002;76:63-73.
Awoyinka OA, Adebawo OO. Quality assessment and potential utilization of high amylolytic Nigerian maize cultivar. Africa Journal Biotechnology. 2008;7:4331- 4335.
Mohammed AM, Addy EO. Hydrolytic enzymes levels in malting cereals. Advances in Biochemistry. 2014;2(5): 76-79.
Ahuja I, Kissen R, Bones AM. Phytoalexins in defense against pathogens. Trends Plant in Science. 2012;17(2):73 90.
Polesani M, Bortesi L. Ferrarini A, Zamboni A, Fasoli M and Zadra C. General and species-specific transcriptional responses to downy mildew infection in a susceptible (Vitis vinifera) and a resistant (V. riparia) grapevine species. BMC Genomics. 2010; 11(117): 1-16.
Malacarne G, Vrhovsek U, Zulini L, Cestaro A, Stefanini M, and Mattivi F. Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses. BMC Plant Biology. 2011; 11:114.
Sanni DM, Fatoki TH. Evaluation of malting properties and activities of three enzymes from sorghum (Sorghum bicolor) during malting. African Journal of Food Science and Technology. 2017;8(6): 090-098.
Ahmed SB, Mahgoub SA, Babiker BE. Changes in tannin and cyanide contents and diastic activity during germination and the effect of traditional processing on cyanide content of sorghum cultivars. Food Chemistry. 1996;56(2):159-162.
Uriyo M and Eigel WE. Duration of kilning treatment on a-amylase, b-amylase and endo-(1,3)(1,4)-b-endoglucanase activity of malted sorghum (Sorghum bicolor). Process Biochemistry. 1999;35:433–436.
Guiga W. Identification of barley germination inhibitors and development of a treatment process for steep water in maltings for recycling. University of Lorraine (France). Thesis. 2006;199.
Trabzuni DM, Ahmed SEB, Abu- Tarboush HM. Chemical Composition, Minerals and Antioxidants of the Heart of Date Palm from Three Saudi Cultivars. Food and Nutrition Sciences. 2014;5: 1379-1386.