Antioxidant Activity of Ethyl Acetate Extract from Endohytic Bacteria of Mangosteen (Garcinia mangostana L.) Roots

Bastian Nova *

Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas, Padang, West Sumatera, Indonesia.

Epi Supri Wardi

Department of Pharmacy, Faculty of Pharmacy, Universitas Perintis Indonesia, Padang, West Sumatera, Indonesia.

Ria Afrianti

Department of Pharmacy, Faculty of Pharmacy, Universitas Perintis Indonesia, Padang, West Sumatera, Indonesia.

Aldo Maihendra

Department of Pharmacy, Faculty of Pharmacy, Universitas Perintis Indonesia, Padang, West Sumatera, Indonesia.

*Author to whom correspondence should be addressed.


Abstract

Aims: This study aims to obtain isolates of endophytic bacteria found in mangosteen roots, determine information regarding the chemical contents of secondary metabolites from endophytic bacteria, and obtain antioxidant activity data using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method.

Place and Duration of Study: The research was conducted from June to September 2019 at the Microbiology Laboratory of Universitas Perintis and the Higher Education Coordinating Board Region X, West Sumatra, Indonesia.

Methodology: Isolation of endophytic bacteria, secondary metabolite production by endophytic bacteria, phytochemical screening, and antioxidant activity testing using the DPPH method.

Results: The results of measuring the antioxidant activity of secondary metabolite extracts from endophytic bacteria of mangosteen rootsusing the DPPH method yielded an IC50 of 14,047.912 g/ml and gallic acid solution of 2,498 g/ml. Furthermore, the T-test analysis between the % inhibition of secondary metabolites from endophytic bacteria of mangosteen roots and gallic acid yielded a significant value of 0.013.

Conclusion: The antioxidant activity of secondary metabolites from endophytic bacteria of mangosteen roots is considered weak and cannot be an alternative or solution for antioxidant production.

Keywords: Mangosteen root, Garcinia mangostana L, antioxidants, endophytic bacteria


How to Cite

Nova, B., Wardi, E. S., Afrianti, R., & Maihendra, A. (2024). Antioxidant Activity of Ethyl Acetate Extract from Endohytic Bacteria of Mangosteen (Garcinia mangostana L.) Roots. Asian Journal of Research in Biochemistry, 14(4), 27–33. https://doi.org/10.9734/ajrb/2024/v14i4291

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