Effects of ‘ZPC’ Polyherbal Formulation on Diabetic-Dyslipidemic Wistar Rats

Main Article Content

Agatemor Uzuazokaro Mark- Maria
Ozioko Eucharia Ngozi
Momoh Theophilus Boniface
Idakwoji Precious Adejoh
Nweje- Anyalowu Paul Chukwuemeka

Abstract

In this study the antidiabetic effect of a polyherbal formulation- ZPC was investigated in Wistar rats. Diabetes was induced by intraperitoneal injection of streptozotocin at a dose of 50 mg/kg. Rats having Fasting Blood Sugar (FBS) level above 250 mg/dl after 72 hrs were considered diabetic and used for the studies. Five rats served as non- diabetic control (Group 1) while twenty diabetic rats were randomized into 4 groups of 5 rats each. The four groups (Groups 2,3,4 and 5) received 1ml (diabetic control), 250 mg/kg chlorpropamide and ZPC at doses of 250 and 500mg/kg respectively for 28 days. During the treatment period, the FBS and bodyweight of rats were monitored weekly and on day 28, the rats were euthanized and blood samples collected for serum lipid profile analysis. Results obtained indicated that following administration of streptozotocin, there was a significant (p<0.05) increase in the FBS, total cholesterol, triglycerides and LDL concentration with a corresponding significant (p<0.05) decrease in HDL concentration compared to non- diabetic control. However, following the treatment with the polyherbal formulation, there was a significant (p<0.05) reduction in the FBS level and a significant (p<0.05) increase in the body weight of rats compared to the diabetic control. The polyherbal formulation also produced a significant (p<0.05) reduction in total cholesterol, triglycerides and LDL concentration with a corresponding significant (p<0.05) increase in HDL concentration compared to diabetic control. It was concluded that, ZPC might serve as a good alternative or as an adjunct to the oral hypoglycaemic agents in the management of diabetes.

Keywords:
Polyherbal, formulation, diabetic, dyslipidemic, wistar rats

Article Details

How to Cite
Mark- Maria, A., Ngozi, O., Boniface, M., Adejoh, I., & Chukwuemeka, N.- A. (2019). Effects of ‘ZPC’ Polyherbal Formulation on Diabetic-Dyslipidemic Wistar Rats. Asian Journal of Research in Biochemistry, 4(3), 1-9. https://doi.org/10.9734/ajrb/2019/v4i330071
Section
Original Research Article

References

Patalet CJ, Watt E. Purification and identification of active components of pobrotus edulis L. J. Ethnopharm. 2011; 76:87-91.

Warjet IJ, Guinko S, Tuquet C, Salle G. Mitletoes of the agroforestry parklands of Burkina Faso. Agroforestry Syst. 2011;60: 39-49.

Chauchan FW, Shieh P, Kuo D, Hsieh C. Evaluation of the antioxidant activity of Ruellia tuberosa. Food Chemistry. 2010; 94:14-18.

Balaraman A, Rial-Sebbag E, Knoppers BM. Trends in ethical and legal frameworks for the use of human biobanks. European Respiratory Journal. 2007;30(2):373–382.

Grover FK, Malaisse WJ, Pipeleers DG. Selective uptake of alloxan by pancreatic B-cells. Biochem J. 2002;208:513-515.

Junget X, Liang W, Mao Y, Li H, Yang Y, Tan H. Hepatic glucokinase activity is the primary defect in alloxan-induced diabetes of mice. Biomed Pharmacother. 2009;63: 180-186.

Richard KB. Dietary carbohydrate restriction in type 2 diabetes mellitus and metabolic syndrome: time for a critical appraisal; 2008.

Rosenzweig S, Reibel DK, Greeson JM, Edman JS, Jasser SA, McMearty KD, Goldstein BJ. Mindfulness-based stress reduction is associated with improved glycemic control in type 2 diabetes mellitus. 2007;13:36-38.

Tsao AS, Kim ES, Hong WK. Chemoprevention of cancer. Cancer Journal. 2004;54:150- 180.

Wang WH, Wang ZM, Studies of commonly used traditional medicine ginger.(2005) Zhongguo Zhong Yao Za Zhi. 2005;30:1569–1573.

Tapsell LC, Hemphill I, Cobiac L. et al. Health benefits of herbs and spices: The past, the present, the future. The Medical Journal of Australia. 2006;185(Suppl.4): S4–S24.

Unander DW, Vankates PC, Waran I. et al. Phyllantus species: Sources of new antiviral compounds. In: J Simons (eds). Advances in new crops. Timber press, Portland; 1990.

Muktar H, Ahmad N. Tea polyphenols: Prevention of cancer and optimizing health. Am. J. Clin. Nutr. 2000;71:1698S 1702S.

Graham HN. Tea. In: Wiley encyclopedia of food science and technology. 2nd ed. Frederick JF, Ed. John Wiley & Sons: New York. 1999;1-4:2292-2305.

Lorke D. A new Approach to Practical Acute Toxicity Testing. Archives of Toxicology. 1983;54(4): 275-287.

Crook MA Clinical Chemistry and Metabolic Medicine. 7th Edn. Hodder Arnold, London: 2006;426.

Jerry RM, Christina NH, Paul FS. Techniques in organic chemistry. Third Editn. W. H. Freeman and Company, 2010;176.

Tyrberg B, Andersson A, Borg LA. Species Differences in Susceptibility of Transplanted and Cultured Pancreatic Islets to the β-Cell Toxin Alloxan. General and Comparative Endocrinology. 2001; 122(3):238–251.

Wassan KM, Najafi S, Wong J, Kwong M. Assessing plasma lipid levels, body weight, and hepatic and renal toxicity following chronic oral administration of a water soluble phytostanol compound FM-VP4, to gerbils. Journal of Pharmaceutical Science. 2001;4(3):228-234.

Murthly KB, Nammi S, Kola MK, Roa KRV, Rao KN, Annapuma A. Evaluation of Hypoglyceamic and Anti-hypoglycaemic effect of Dasura metel (linn) seeds in normal and alloxan–induced Diabetic rats. Journal of Ethnopharmacology. 2004;91: 95-98.

Kameswara MA, Mathew L, Radha A. A report on the antioxidant activities of leaves and rhizomes of Costus pictus D. Don. International Journal of Integretive Biology. 1997;5(1):20-26.

Rai SA, Larijani B, Akhoondzadeh S, Fakhrzadeh H, Dastpak A, Rezai A, Bandarian F, Badi HN, Emami T. J. Ethnopharmacol. 1994;14:202.

Eleazu H, Peschke D, Bromme HJ, Morke W, Blume R, Peschke E. Influence of melatonin on free radical-induced changes in rat pancreatic beta-cells in vitro. J Pineal Res. 2000;28:65-72.

Idakwoji PA, Akuba OB, Elah OS. Co-Administration of Ethanolic Leaf Extract of Moringa Oleifera and Metformin Reverses Polyphagia, Polydipsia and Stabilizes Body Weight in Alloxan- Induced Diabetic Rats; Global J Res. Med. Plants & Indigen. Med. 2015;9:193–202.

Gerich JE. Physiology of glucose homeostasis. Diabetes Obesity and Metabolism. 2000;2:345–350.

Idakwoji PA, Okafor SC, Akuba OB, Ajayi OI, Hassan SM. In vivo and in vitro comparative evaluation of the anti-diabetic potentials of the parts of Moringa oleifera tree. European Journal of Biotechnology and Bioscience. 2016;4(1):14-22.

American Diabetic Association. Standards of medical care in diabetes. Diabetes Care. 2000;33(1):S11–S61.

Champe P, Harvey R, Ferrier D. Lipid metabolism. Lippicott’s Illustrated Review: Biochemistry. Indian edition, Jaypee Brothers: Medical Publisher (P) Ltd.: 2005; 171–217.

Rotimi SO, Omotosho OE, Roimi OA. Persistence of acidosis in alloxan induced diabetic rats treated with the juice of Asystasia gangetica leaves. Phcog. Mag. 2011;7:25-30.

Claudia ENM, Julius EO, Dagobert T, Etienne D. Antidiabetic and hypolipidemic effects of Laportea ovalifolia (Urticaceae) in alloxan-induced diabetic rats. Afr. J. Trad. Complementary Alternative Med. 2006;3:36-43.

Daisy P, Lily V, Cecelia EP. Comparative Studies on the Different Leaf Extract of Elephanto pusscaber. L. on Streptozocin-Induced Diabetic Rats. European J. Scientific Res. 2009;32:304-313.

Eze ED, Mohammed A Musa KY, Tanko Y, Isa AS. Effect of ethanolic leaf extract of Mucuna pruriens (fabaceae) on lipid profile in alloxan-induced diabetic Wistar rats. British Journal of Pharmacology and Toxicology. 2012;3:102- 109.

Idakwoji PA, Salawu OA, Maiha BB, Obidike I, Tijani AY. Co-administeration of Ethanolic Leaf Extract of Moringa oleifera and Metformin Improves Glucose, Lipid and Protein Profiles of Diabetic Wistar rats. Biokemistri. 2015;27(3):123–138.

Idakwoji PA, Salawu OA, Maiha BB, Obidike I, Tijani AY. Assessment of biochemical and histopathological changes in diabetic wistar rats co-administered ethanolic leaf extract of moringa oleifera and metformin. International Journal of Biological & Pharmaceutical Research. 2015;6(12):1008-1019.

Mathe JD. Dyslipidemia and diabetes animal models. Mettab. 1995;21: 106.

Nimenibo-uadia R. Effect of aqueous extract of Canavalia ensiformis seeds on hyperlipidemic and hyperkotonaemia in alloxan-induced diabetic rats. Biokemistri. 2003;15:7-15.

Suryawanshi NP, Bhutey AK, Nagdeote A N, Jadhav AA, Manoorkar GS. Study of lipid peroxide and lipid profile in diabetes mellitus .Indian J. Clinic. Biochem. 2006;1: 126-130.

Libby P. Current concepts of the pathogenesis of the acute coronary syndromes. Circulation. 2011;104:365-372.

Oyewo EB, Akanji MA. Immune modulation potentials of aqueous extract of Andrographis paniculata leaves in male rat. Researcher. 2010;5:928–935.

Idakwoji PA, Akuba OB, Okafor SC. Comparative Anti-radical Activity of Five Indigenous Herbal Plants and their Polyherbal Extract International Journal of Biochemistry Research & Review. 2016; 11(1):1-10.

Idakwoji PA, Uzuazokaro MMA. Anti- radical and Inhibitory Effect of some Common Nigerian Medicinal Plants on Alpha Glucosidase, Aldose Reductase and Angiotensin Converting Enzyme: Potential Protective Mechanisms against Diabetic Complications. Int. J. Adv. Res. Biol. Sci. 2018;5(3):188-201.