Biochemical Parameters, Activity Levels of Marker Enzymes and Serum Electolytes of Wistar Albino Rats Fed Mango (Mangifera indica) Pulp Formulated Diets

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Pauline N. Iheagwam
Eugene N. Onyeike
Benjamin A. Amadi


Biochemical parameters, activity levels of marker enzymes and serum electrolytes of Wistar albino rats fed naturally ripe (control), unripe and artificially ripened mango pulp formulated diets were investigated. Ripe and unripe fruits were collected and used for the study and artificially ripened mangoes were obtained by wrapping unripe fruits with dark polyethylene bag; treatment with calcium carbide and by dipping into hot water. After ripening, the mango samples were air-dried, pulverized and used to formulate the 10%, 20%, 30% diets fed to rats for 28 days and their plasma collected for biochemical investigations. At 10% level of incorporation, plasma protein concentration in the control (65.63±0.12 mg/dl) was significantly higher (p< 0.05) than values for unripe groups (55.50±1.91 mg/dl) and calcium carbide (56.97±1.27 mg/dl) while Albumin concentration was highest in the unripe (41.33±3.58 mg/dl) but lowest in the calcium carbide (33.50±0.69 mg/dl) but at 20 and 30% levels, no significant difference was observed for both parameters. Billirubin was significantly higher in the calcium carbide groups but lowest in the control groups at all levels of incorporation while Creatinine and Urea revealed no significant difference at all levels. Marker enzyme assay showed significant variations only in the concentrations of Alkaline phosphatase and Gamma glutamyl transterase at the 30% level of incorporation only. Electrolyte assay revealed that at 30% level of incorporation, significantly lower levels of potassium were observed in the calcium carbide (3.43±0.06 mg/dl) group compared to the control (3.80±0.17 mg/dl).Thus, artificial ripening of fruits may adversely affect Biochemical parameters, activity levels of marker enzymes and serum electrolytes.

Artificial ripening, mango, enzyme markers, biochemical parameters, electrolyte profile

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Iheagwam, P., Onyeike, E., & Amadi, B. (2019). Biochemical Parameters, Activity Levels of Marker Enzymes and Serum Electolytes of Wistar Albino Rats Fed Mango (Mangifera indica) Pulp Formulated Diets. Asian Journal of Research in Biochemistry, 4(3), 1-10.
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Mauseth JD. Botany: An introduction to plant biology. Jones and Bentlett Press. 2003;271-272.

Hayes DP. Protective role of fruits and vegetables against radiation induced cancer. Nutritional Review. 2005;63(9):303-11.

Rossato SB, Haas C, Raseira MC, Moreira JC, Zuanazzi JA. Antioxidant potential of peels and fleshes of peaches from different cultivars. Journal of Medicinal. Food. 2009;12(5):1119-26.

Onyeani CA, Osunlaja OS, Sosanya OS, Oworu OO. Mango fruit anthracnose and the effects on mango yield and market values in Southwestern Nigeria. Asian Journal of Agricultural Research. 2012;6:171-79.

Farina V, Mazzaglia A, Padoan D, Barone F. Chemical-physical and sensory characterization of mango fruits (Mangifera indica L.) cultivated in Sicily (Italian). Acta Hortus. 2013;12:110-13.

Jacka FN, Kremer PJ, Leslie ER, Berk M, Patton GC, Toumbourou JW. Associations between diet quality and depressed mood in adolescents: Results from the Australian healthy neighbourhoods study. Australian and New Zealand Journal of Psychiatry. 2010;44(5):435-42.

Akbaraly TN, Sabia S, Shipley MJ, Batty GD, Kivimaki M. Adherence to healthy dietary guidelines and future depressive symptoms: Evidence for sex differentials in the Whitehall II study. The American Journal of Clinical Nutrition. 2013;97(2):419-27.

Oyebode O, Gordon-Dseagu V, Walker A, Mindell JS. Fruit and vegetable consumption and all-cause cancer and CVD mortality: Analysis of health survey for England data. Journal of Epidemiology and Community Health. 2014;68(9):856-62.

Allgo WA, Wardle J, Steptoe A. Depressive symptoms, social support, and personal health behaviors in young men and women. Health Psychology. 2001;20(3):223-24.

Adams TB, Colner W. The association of multiple risk factors with fruits and vegetable intake among a nationwide sample of college students. Journal of American College Health. 2008;56(4):455-61.

Amarakoon R, Illeperuma DCK, Sarananda KH. Effect of calcium carbide treatment on ripening and quality of Velleicolomban and Willard mangoes. Tropical Agricultural Research. 1999;11:54-60.

Goldmann IL, Kader AA, Heintz C. Influence of production, handling, and storage on phytonutrient content of foods. Nutritional Review. 1999;57:46-52.

Gbakon SA, Ubwa TS, Ahile UJ, Obochi O, Nnannadi I, Yusufu A, Ikagu M. Studies on changes in some haematological and plasma biochemical parameters in wistar rats fed on diets containing calcium carbide ripened mango fruits. International Journal of Food Science and Nutrition Engineering. 2018;8(2):27-36.

Harborne JB. Phytochemical methods. Chapman and Hall Ltd, London. 1993;279.

Igbinaduwa P, Aikpitanyi-Iduitua R. Calcium carbide induced alterations of some haematological and serum biochemical parameters of wistar rats. Asian Journal of Pharmaceutical and Health Sciences. 2016;6(1):1360-70.

Kjuus HA, Andersen S, Langard. Incidence of cancer among workers producing calcium carbide. Porsgrunn and the Cancer Registry of Norway. 2 Montebello, Oslo 3, Norway; 2007.

Iroka CF, Akachukwu EE, Adimonyemma RN, Nkumah CO, Nwogiji CO. Effects of induced ripening on the proximate, biochemical and mineral compositions of Carica papaya (Pawpaw Fruit). European Journal of Medicinal Plants. 2016;15(3):1-10.

Kader AA. Post harvest techno. Hort. Crops. Univ. of California. 2002;157.

Dhembare AJ. Bitter truth about fruit with reference to artificial ripeneing. Archives of Applied Science Research. 2013;5(5):45-54.

Olisah MC, Ilechukwu OU, Ifemeje JC, Ofor CC. Effect of vitamin C against calcium carbide induced hepatoxicity. Tropical Journal of Applied Natural Sciences. 2017;2(1):74-77.

US National Institute of Health. Guide for the care and use of laboratory animals, (8th Ed). The National Academic Press, Washington DC, USA; 2011.

Tietz NW, Prude EL, Sirgard-Anderson O. Tietz textbook of clinical chemistry. (2nd Ed). W. B. Saunders Company, London. 1994;1354-137.

AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. Washington D.C.; 1990.

Appiah F, Kumah P, Idun I. Effect of ripening stage on composition, sensory qualities and acceptability of Keitt mango (Mangifera indica) chips. Ajfand Scholarly Peer Reviewed. 2011;11(5):5096-108.

Ogbuagu DH, Adebayo ET, Ayoade AA, Ugwu OB, Mba DO. Lead accumulation in and its haematological effects on African catfish, Clarias gariepinus. African Journal of Aquatic Science. 2015;40(2):1-4.

Sogo-Temi CM, Idowu OA, Idowu E. Effect of biological and chemical ripening agents on the nutritional and metal composition of banana (Musa spp). Journal of Applied Science and Environmental Management. 2014;18(2):243-46.

Banadda N, Namawejje H, Ayaa F, Kigozi JB, Sendagi S. Diffusive flux modeling of lead migration from black polythene bags into food: A case study of green bananas (Matooke). African Journal of Food Science. 2011;5(5):313-19.

Gatsing D, Aliyu R, Kuiat,e JR, Garba IH, Jaryum KH, Tedongma N, Tchouanguep MF, Adoga GI. Toxicological evaluation of the aqueous extract of Allium sativum bulbs on laboratory mice and rats. Cameroon Journal of Experimental Biology. 2005;1:39-45.

Cheesbrough M. District laboratory practice in tropical countries, part 2. Cambridge University Press, Cambridge; 2006.

Halsted JA, Halsted CH. The laboratory in clinical medicine: Interpretation and application, @ (nd ed). WB. Saunders Company, Philadelphia. 1991;281-283.

Dara L, Hewett J, Lim JK. Hydroxycut hepatotoxicity: A case series and review of liver toxicity from herbal weight loss supplements. World Journal of Gastroenterology. 2008;14(45):6999-004.

Obochi GO, Ikewebe J, Ogli SA, Oloche JJ. Biochemical and haematological changes associated with ethanol-induced gastric lesions in wistar rats. Journal of Medical Sciences. 2015;15(20):100- 104.

Savithri Y, Sekhar PR, Doss PJ. Changes in haematological profiles of albino rats under chlorpyrifos toxicity. International Journal of Pharmacological Biological Sciences. 2010;1:1-7.

Adebayo AH, Tan NH, Akindahunsi AA, Zeng GZ, Zhang YM. Anticancer and antiradical scavenging activity of Ageratum conyzoides L. (Asteraceae). African Journal of Biotechnoogy. 2010;6:62-66.

Hossain MF, Akhtar S, Anwar M. Health hazarads posed by the consumption of artificially ripened fruits in Bangladesh. International Food Research Journal. 2015;22(5):1755-60.

Cheesbrough M. District laboratory practice in tropical countries, part 1. Cambridge University Press, Cambridge; 1998.

Aliyu R, Adebayo AH, Gatsing D, Garba IH. The effects of ethanolic leaf extract of Commiphora africana (Burseraceae) on rat liver and kidney functions. Journal of Pharmacology and Toxicology. 2007;2: 373-379.

Dhembare AJ, Gholap AB. Modulation in serum biochemicals in European rabbit, Oryctolaguscuniculus (Linn.) exposed to ethephon. European Journal of Experimental Biology. 2012;2(3):794-9.

Sarac F, Saygilis F. Causes of high bone alkaline phosphatase. Biotechnology and Biotechnological Equiptment. 2007;21(2): 194-97.

Young EC, Ria AR, Lomeda SR, Ho-Yong S, Hong-In SJ, Beattie H, In-Sook K. Zinc deficiency negatively affects alkaline phosphatase and the concentration of Ca, Mg and P in rats. Nutrition Research and Practice. 2007;1(2):113-19.

Giannini E, Domenico R, Federica B, Bruno C, Alberto F, Federica M, et al. Validity and clinical utility of the Aspartate aminotransferase - Alanine aminotransferase ratio in assaying disease severity and prognosis in patients with hepatitis C virus-related chronic liver disease. Arch Intern Medicine. 2003;163(2):218-24.

Orhue NEJ, Nwanze EAC, Okafor A. Serum total protein, albumin and globulin levels in Trypanosoma brucei-infected rabbits: Effect of orally administered Scoparia dulcis. African Journal of Biotechnology. 2005;4(10):1152-55.

Nillena MS. Fresh fruits are nutritious, but artificial ripening poses health hazards. The Hindu. 2012;5.

Policegoudra RS, Aradhya SM. Biochemical changes and antioxidant activity of mango ginger (Curcuma amada Roxb) rhizomes during postharvest storage at different temperatures. Postharvest Biology and Technology. 2007;46(2):189-94.

Esfeh JM, Hanouneh IA, Zein NN. A 25-year old man with very high phosphates. Cleve Clin Journal of Medicine. 2011;78(12):793-00.

Dike HO, Cosmas O. Calcium carbide-induced haematological alterations in the albino mice- Mus musculus. Journal of Environmental Science, Toxicology and Food Technology. 2016;10(10):100-04.