Study of the Variation of Some Biochemical Parameters in Rattus norgevicus

Main Article Content

Obou Constantin Okou
Djako Sosthène Thierry Akré
Gomé Michel Bleu
Assandé Francis Ehounou
Alain Dit Philippe Bidié

Abstract

The biochemical assessment in animals is of capital interest to define the diagnosis of many diseases. The variation of biochemical parameters (urea, glycemia, creatinemia, transaminases: GPT and GOT, total cholesterol, HDL-c, LDL-c, triglycerides and total lipids) is an indicator of the state of health of certain vital organs such as the kidney, liver, pancreas, veins, heart, etc... . For this study, to be able to determine the normal values of the rats, a set of batches of male and female Wistar rats was formed and acclimatized for 2 months. Each batch set consisted of thirty-nine (39) rats. Each rat was weighed and then sampled by venipuncture at the caudal level to collect their blood in a dry tube (red). The red tube was used to determine the biochemical parameters. The results of this study showed that some animals already had certain pathologies before the use of any beneficial or harmful substance on them. However, most of the animals used have normal biochemical parameters revealing the good state of their various vital functions (kidney, liver, heart, veins, pancreas, etc.). These values of determining standard biochemical parameters can form a basis for future tests.

Keywords:
Rats, biochemical parameters, normal values, pathologies.

Article Details

How to Cite
Okou, O. C., Akré, D. S. T., Bleu, G. M., Ehounou, A. F., & Bidié, A. D. P. (2020). Study of the Variation of Some Biochemical Parameters in Rattus norgevicus. Asian Journal of Research in Biochemistry, 7(4), 21-34. https://doi.org/10.9734/ajrb/2020/v7i430145
Section
Original Research Article

References

Gupta AR, Patra RC, Saini M, Swarup D. Haematology and serum biochemistry of chital (axis axis) and barking deer (Muntiacus muntjak) reared in semi-captivity. Vet Res Comm. 2007;31:801- 08.

Yapo AE., Assayi MJ, Aka B, Bonetto R, Lonsdorfer A, Comoé L et al. The reference values of 21 blood biochemical constituents of presumed healthy Ivorian adults. African pharmacy. 1989;44:13-21.

Boum B, Tantchou J. Biochemical standards of Cameroonian in the region of Yaoundé. Science and Technical Review. 1985;11(1):103-7.

Acker P, Maydat L, Trapet P. Quelques constantes biochimiques actuelles de l’africain congolais normal. Bull Soc Pathol. 1987;1:460-7.

Siby S. Study of the variation of biochemical and hematological parameters in the district of Bamako. State Thesis in Medicine. Faculty of Medicine, Pharmacy and Odonto-Stomatology, University of Bamako, Mali. 2008;77.

Sandé J, Coulibaly JL, Njikeutchi F, Bouabré A, Boukary A, Guissou IP. Establishment of reference values for 15 biochemical blood constituents in adults from Burkina Faso in Ouagadougou (Burkina-Faso). Annals of Clinical Biology. 2004;62:229-34.

Vincent-Viry M, Henny J, Clerc M, Siest G. Discussion of some “Reference limits” of European and African populations. (Practical conclusions. International cooperative study). Med Afri Noire. 1987;34(5):459-465.

Popoff M. Biochemical data in sheep: application to the differential diagnosis of some metabolic diseases. Bull Soc vét prat Fr. 1981;9:695-06.

Obi TU, Oduye OO. Haematological changes in natural and experimental peste des petits ruminants virus infection in goats. Rev high med vet country too. 1985;38:11-5. Available:https://doi.org/10.19182/remvt.8534

Ndoutamia G, Ganda K. Determination of haematological and biochemical parameters of small ruminants in Chad. Rev Med Vet. 2005;156(4):202-06.

Descat F. Rat haematology: Haemogram and myelogram. Exercise thesis, National Veterinary School of Toulouse – ENVT. 2002;110.

Sakandé J, Ahiboh H, Edjemé A, Yapo AE. Study of the biological tolerance of a plant with antiplasmodial activity Momordica charantia (Cucurbitaceae). Mali Medical. 2003;XVIII(1-2):1-4.

Farougou S, Kpodekon M, Loko F, Brahi OHD, Agniwo.B, Djago.Y. Usual values of the main serum biochemical parameters in rabbits (Oryctolagus cuniculus) reared in Benin. RASPA. 2007;5(1-2):17-22.

Fasce CF. Serum Cholesterol determined colorimetrically with enzyme. Clin Chem. 1982;18:901.

Bouiddouh FZ. Evolution of serum biochemical parameters in Wistar rats treated with an ethanolic extract of colocynth (Citrullus colocynthis) seeds. Master's thesis in Biology Option: Applied Biochemistry, Faculty of Nature and Life Sciences and Earth and Universe Sciences, Abou Bakr Belkaid-Tlemcen University, Algeria. 2012;63.

Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem. 1982;28:2077-80. DOI: 10.1093 /clinchem / 28.10.2077

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of Low Density Lipoprotein Cholesterol in plasma, Without use of the preparative ultracentrifuge. Clin Chem. 1972;18:499-02. Available:https://doi.org/10.1093/clinchem/18.6.499

Djeffal A. Evaluation of the toxicity of a carbamate insecticide "methomyl" in Wistar rats: Oxidative stress and exploration of the protective effects of selenium and / or vitamin C supplementation. 3rd Cycle Thesis, Option: Applied Biochemistry. Faculty of Sciences, Badji Mokhtar-Annaba University, Algeria. 2014;170.

Amotte M. Introduction to statistical methods in biology 2th ed. Paris: Masson et Cie; 1971.

Schwartz D. Statistical Methods for the Use of Physicians and Biologists. 3th ed. Paris: Flammarion medicine-Sciences; 1992;311.

Diaby V. Biochemical, hematological and histological studies of cadmium sulfate in Wistar rats. Doctoral Thesis, Specialty: Functional and Molecular Biology, UFR Biosciences, Félix Houphouët-Boigny University, Abidjan, Côte d'Ivoire. 2017; 130.

Grenier-Michaud S, Lyne C, Pierre N. Understanding renal function is an essential component of paraclinical surveillance. Nurse perspective. 2011;30-5.

Pitel Ph, Moulin M, Valette J-P, Dumontier S, Petit L, Fortier G, et al. Approach to haematological and biochemical values in two asina breeds. Equine Veterinary Practice. 2006;38(149):19-25.

Pritchard JC, Burn CC, Barr ARS, Whay HR. Haematological and serum biochemical reference values for apparently healthy working horses in Pakistan. Res Vet Sci. 2009;87:389-95. DOI: 10.1016 / j.rvsc.2009.05.003

Zabré ZM. Determination of the usual biochemical parameters in small ruminants in Burkina Faso and their variations in subjects naturally infected with trypanosomiasis. Veterinary Thesis, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar, Senegal. 2013;71.

Abenga JN, Anosa VO. Serum total proteins and creatinine levels in experimental Gambian trypanosomosis of vervet monkeys. Afr J Biotechnol. 2005;4:187-90.

Hilali M, Abdel-Gawad A, Nassar A, Abdel-Wahab A. Hematological and biochemical changes in water buffalo calves (Bubalus bubalis) infected with Trypanosoma evansi. Vet Parasitol. 2006;139:237-43. DOI: 10.1016 / j.vetpar.2006.02.013

Isler C. Evolution of biochemical parameters during left displacement of the abomasum in dairy cows: Study of four cases. Veterinary Thesis, National Veterinary School, Claude-Bernard University, Lyon I, France. 2007;111.

Kim WR, Flamm SL, Di Bisceglie AM, Bodenheimer HC Jr. Serum activity of alanine aminotransferase (ALT) as an Indicator of health and disease. Hepatol. 2008;1363-70. DOI: 10.1002/hep.22109

Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals. 5th ed. Academic Press, San Diego, California. 1997;932.

Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and monitoring of hepatic injury II. Recommendation for use of laboratory tests in screening, diagnosis and monitoring. Clin Chem. 2000;46:2050- 68. DOI: 10.1093 / clinchem / 46.12.2050

Gomé MB, Kouakou K, Touré A, Traoré F. Study of the acute and subchronic toxicity of aqueous extract of Passiflora foetida Linn. (Passifloraceae) in rats and mice. IJBCS. 2011;5(5):1777-89. DOI: http://dx.doi.org/10.4314/ijbcs.v5i5.1

Pratt DS, Kaplan MM. Evaluation of abnormal liver-enzyme results in asymptomatic patients. N Engl J Med. 2000;342:1266-71.

Al-Habori M, Al-Aghbari A., Al-Mamary M., Baker M. Toxicological evaluation of Catha edulis leaves: a long-term feeding experiment in animals. J Ethnopharmacol. 2002;83:209-17. DOI: 10.1016/S0378-8741(02)00223-4

Al-Shinnawy M. Vitamin C as ameliorative agent against thiodicarb toxicated male albino rats (Rattus norvegicus). Egypt Acad J Biol Sci. 2008;1(2):177-87.

Eastham RD. Abbreviated as biological constants. ed. Masson: Paris, France. 1978;248.

Heuillet M. Development of reference methods for biomarkers of lipid balance: application to quality control in clinical biology. Doctoral Thesis, Discipline: Life Sciences, University of Burgundy, Paris, France. 2013;199.

Lahoz C, Mostaza JM. Atherosclerosis as a systemic Disease. Rev Esp Cardiol. 2007;60(2):184-95. DOI: 10.1016/S1885-5857(07)60131-5

Bidié ADP, Adeoti FM, Yapo FA, Tiekpa JW, N’guessan JD, Djaman JA. Effect of total aqueous extract of Chrysophyllum perpulchrum on hematological, biochemical parameters and weight growth in wistar healthy rats. REVIST. 2016;28:33-348.

Lewington S, Whitlock G, Clarke R, Sherliker P, Emberson J, Halsey J et al. Blood cholesterol and vascular mortality by age, sex, and blood pressure: A meta-analysis of individual data from 61 prospective studies with 55,000 vascular deaths. Lancet. 2007;370:1829-39. DOI: 10.1016/S0140-6736(07)61778-4

Gervois P, Balduyck M, Brousseau T. Chapter 10: Cardiovascular disease: markers of atherosclerosis, coronary heart disease and stroke. In: Durand G, Beaudeux J-L. 2th ed. Medical biochemistry: current markers and perspectives. EM inter. 2008;99-114.