Changes in Some Biochemical Parameters and Body Weights of Healthy Albino Rats Following Palm-wine Administration

Main Article Content

G. S. Haruna
O. B. Nwaikuku
B. A. James
N. Musa
R. Valentine

Abstract

Aims: To evaluate the effect of palm-wine administration on liver function, antioxidant parameters and body weights in albino rats.

Study Design: Randomized block design.

Place and Duration of Study: Department of Biochemistry and Molecular Biology Laboratory, Nasarawa State University, Keffi, Nasarawa State, Nigeria between June and August, 2019.

Methodology: Twenty adult male albino rats were divided in to five groups of four rats each, group 1 was the control. The experimental groups were administered fresh and prolonged-fermented palm-wine thus; Group 2: 15ml/ of fresh palm wine, Group 3; 15ml/ kg of prolonged fermented palm wine, Group 4; 30ml/ kg of fresh palm wine, Group 5; 30 ml/ kg of prolonged fermented palm wine, for 28 days.

Results: AST activity decreased significantly (P<0.05) in groups 3 (9.615±0.225), 4 (9.860±0.162) and 5 (9.390±0.624) when compared to the control (10.505±0.121). ALT activity was observed to decrease significantly (P<0.05) in group 5 (7.415±0.548) when compared to control. ALP decreased significantly (P<0.05) in group 3 (7.395±0.710) when compared to control (8.250±0.577). Total Bilirubin, was significantly (P<0.05) lower in groups 3 (0.120±0.041) and 5 (0.170±0.012) compared to control (0.650±0.012). MDA decreased significantly (P< 0.05) in groups 2, 3 and 5 compared to control. SOD decreased significantly (P<0.05) in group 2 (2.735±0.110) compared to control (2.990±0.012). Groups 4 (2.700±0.046) and 5 (2.710±0.346) showed significant (P< 0.05) decrease in GPX when compared to control. GSH was significantly (P< 0.05) higher in groups 4 (5.950±0.058) and 5 (5.925±0.064) compared to control (3.110±0.035). By the third week, the mean body weights of the rats significantly (P<0.05) decreased in all the groups compared to week 2.

Conclusion: Palm-wine did not exert remarkable toxic effect on the liver biomarkers and may not have induced oxidative stress but reduced body weights of the rats in the third week of administration.

Keywords:
Palm-wine, biochemical parameters, antioxidants, body weights, evaluation.

Article Details

How to Cite
Haruna, G. S., Nwaikuku, O. B., James, B. A., Musa, N., & Valentine, R. (2020). Changes in Some Biochemical Parameters and Body Weights of Healthy Albino Rats Following Palm-wine Administration. Asian Journal of Research in Biochemistry, 7(4), 35-44. https://doi.org/10.9734/ajrb/2020/v7i430146
Section
Original Research Article

References

Oyedeji KO, Bolarinwa AF, and Hamzat MA. Effect of palm wine on reproductive functions in male albino rats. Research Journal of Biological Sciences. 2012;4:340-344.

Uzogara SG, Agu LN, Uzogara EO. A Review of traditional fermented food condiments and beverages in Nigeria: Their benefits and possible problems. Ecological food Nutrients.1991;24:267-288.

Uzochukwu BUA, Balogh FE, and Ngoddy PD. Standard pure culture inculums of natural fermented palm sap. Nigerian Journal of microbial. 1991;9:67-77.

Amoa-Awua WK, Sampson E and Tano-Debrak K. Growth of yeasts, lactic and acetic acid bacteria in palm wine during tapping and fermentation from felled oil palm in Ghana. Journal of Applied Microbiology. 2007;102:599–606.

Lasekan O. Enantiomeric differentiation of three key volatile compounds in three different palm wines (Elaeis guineensis, Borassusflabellifer and Nypa fruticans). J. of Food. 2017;16(1):70-75.

Detto K, N'Dede TD, Jean- Luc AM, Koffi MJ and Koffi MD. Biochemical and microbial properties of palm wine: Effect of tapping length and varietal differences. Food and Nutrition Scientists. 2016;7(9):23-25.

Reitman S, Frankel S. A method for the determination of serum glutamic oxaloacetate transaminase and serum glutamic pyruvic transaminase. Am. J. Clin. Pathol. 1957;28:56-63.

Rec G. Colorimetric method for serum alkaline phosphatase determination. J. Clin.Biochem. 1972;10(20):182-182.

Jendrassik L, Grof P. Estimation of total serum bilirubin level by spectrophotometrically in serum and plasma. Biochem. Zeitschrift. 1938;297:81-89.

Sherlock S. Liver disease determination of total and direct bilirubin, colorimetric method.1951 London: Churchill, London; 1951.

Wallin B, Rosengren B, Sherlzer, HG, and Camejo G. Lipoprotein oxidation and measurement of TBARS formation in single microliter plate; its use for evaluation of antioxidants. Analytical Biochemistry.1993;208:10-15.

Habig WH, Pabst MJ and Jakoby WB. Glutathione S- transferase, the first step in mercapturic acid formation. J.Biol. Chem.1974;249:7130-7139.

Arthur JR, Boyne R. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper cattle. life Science. 1985;36:1569-1058.

Paglia DE, Valentine WN. Glutathione peroxidase determination. J.Lab. Clin. Med. 1967;70:158-169.

Nwokeke N. Palm wine preservation using traditional plant that preservative bases B.Sc. Thesis.Imo State University Owerri, Nigeria; 2001.

Yurika O, Akira O, Norihiko A and Akifumi O. Effects of vitamin C and E on toxic action of alcohol on partial hepatectommy inducedliover regeneration in rats. J. Clin. Biochem. Nutr. 2018;63 (1):50-57.

Skrydlewska E, Farbiszewski R. Lipid peroxidation and antioxidant status in the liver, erytrocytes, and serum of rats afterb methanol intoxication. J. Toxicol. Environ.Health. 1998;53:88-89.

Martinello F, Da Silva EL. Ascorbic acid interference in the measurement of serum biochemical parameters: In vivo and in vitro studies. J. Clin. Biochem. 2006;24:22-23.

Qing L, Jiu-Hong K and Rong-Liang Z. NADPH oxidase produces reactive oxygen species and maintains survival of rat astrocytes. Cell Biochemistry and Function. 2005;23:93-100.

Duarte TL, Lunec J. Review: When is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radic. Res. 2005;39(7):671-686.

Dinu V, Zamfir O. Oxidative stress in ethanol intoxicated rats. Rev. Rouum. Physiol. 1991;28(1-2):63-67.

Ajiboye TO, Yakubu, MT, Oladiji AT. Electrophilic and treactive oxygen species detoxificastion potentials of chalcone dimers is mediated by Nrf-2. J. Biochem. Mol. Toxicol. 2014;28:11-22.

Chandra R Aneja R, Rewal C, Kondud R, Dass SK, Agarwasl S. An opium alkaloid- Papaverine ameliorates ethanol induced hepatotoxicity: diminution of oxidative stress. Ind. J. Clin. Biochem. 2000;15(2):155-160.

Umoren EB, Obembe AO, Osin EE. Chronic administration of the antiretroviral nevirapine increases body weight, food, and water intake in albino wistar rats. Journal of basic clinical physiological pharmacology. 201232(32): 89-92.

Ganong W. Physiology of growth. In: Review of medical physiology (22nd ed.). Lange medical books/ MC graw hill. Medical publishing division; 2005.

Hoffbrand AV, Moss PA, Pettit JE. Essential haematology. Oxford: M.A: Blackwell publishing Inc; 2006.

Swathy SS, Panicker S and Indira M. Effect of exogenous solanium on the testicular toxicity induced by ethanol in rats. Indian J. physiology and pharmacology. 2006;50(30):215-224.

Theophilus MI, Gabriel IO, Izuchukwu SO. Effect of preservativation methods of oil palm (Elaeis guineensis) sap on health status of male wistar rats. American Journal of Food science and technology. 2015;3(5):137-144.