Evaluation of Bactericidal and Fungicidal Efficacy of Strychno spotatorum Linn. (Nirmali) Seeds
Asian Journal of Research in Biochemistry,
Aim: Diseases due to pathogenic microbes pose a great burden on human health and they have been correlated with socioeconomic, environmental, and ecological factors. The threat due to infectious diseases is further intensified by the continued emergence of new and multidrug resistant microorganisms. This scenario warrants a continuous search for antimicrobial agents preferably of plant origin due to their availability, accessibility, and affordability. The present study was aimed to evaluate the antibacterial and antifungal properties of Strychnos potatorum Linn (Nirmali) seeds using common pathogenic bacterial and fungal strains.
Methodology: Fresh and matured S. potatorum seeds were used for the present study. The powdered seeds were delipidated with petroleum ether (60-80°C) overnight and the extract was filtered. Soxhalation was performed with 95% ethanol to extract the phyto-ingredients from the seeds. Four Gram positive, four Gram negative, and eight fungal strains were used. The antimicrobial activity was evaluated by disc diffusion and well diffusion methods. The Minimum inhibitory concentration (MIC) Minimum bactericidal concentrations (MBC), Minimum fungicidal concentration (MFC) were assayed.
Results: The data obtained through the disc diffusion, well diffusion, the minimum bactericidal concentration, and minimum fungicidal concentrations revealed that the ethanolic extract of the seeds possesses significant antibacterial and antifungal activities. The results obtained were compared with standard drugs widely prescribed for antimicrobial therapy.
Conclusion: The present study provides the scientific rationale for the use of Strychnos potatorum seeds in traditional medicine and a rich source of phytochemicals having significant antimicrobial activities.
- Strychnos potatorum
- antimicrobial activity
- minimum inhibitory concentration
- minimum bactericidal concentrations
- minimum fungicidal concentration.
How to Cite
Mani RS, Ravi V, Desai A, Madhusudana SN. Emerging viral infections in India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2012;82(1):5-21.
Sarma N. Emerging and re-emerging infectious diseases in South East Asia. Indian Journal of Dermatology. 2017;62(5): 451.
Nii-Trebi NI. Emerging and neglected infectious diseases: Insights, advances, and challenges. BioMedresearch International; 2017.
Dobson AP, Carper ER. Infectious diseases and human population history. Bioscience. 1996;46(2):115-26.
Anderson RM, May RM. Population biology of infectious diseases: Part I. Nature. 1979; 280(5721):361-7.
Walker MJ, Barnett TC, McArthur JD, Cole JN, Gillen CM, Henningham A, Sriprakash KS, Sanderson-Smith ML, Nizet V. Disease manifestations and pathogenic mechanisms of group A Streptococcus. Clinical Microbiology Reviews. 2014;27(2): 264-301.
Racaniello VR. “Emerging infectious diseases,” The Journal of Clinical Investigation. 2004; 113,(6):796–798.
Tibayrenc M. Encyclopedia of Infectious Diseases: Modern Methodologies, John Wiley & Sons; 2007.
Carruthers VB, Cotter PA, Kumamoto CA. Microbial pathogenesis: Mechanisms of infectious disease. Cell Host & Microbe. 2007;2(4):214-9.
Rao CD, Yergolkar P, Shankarappa KS. Antigenic diversity of enteroviruses associated with nonpolio acute flaccid paralysis, India, 2007–2009. Emerging Infectious Diseases. 2012;18(11):1833.
Arunkumar G, Vandana KE, Sathiakumar N. Prevalence of measles, mumps, rubella, and varicella susceptibility among health science students in a University in India. American Journal of Industrial Medicine. 2013;56(1):58-64.
Yadav PD, Patil DY, Shete AM, Kokate P, Goyal P, Jadhav S, Sinha S, Zawar D, Sharma SK, Kapil A, Sharma DK. Nosocomial infection of CCHF among health care workers in Rajasthan, India. BMC Infectious Diseases. 2016; 16(1):624.
Saratha V, Subramanian SP. Evaluation of antifungal activity of Calotropis gigantea latex extract: An In vitro study. International Journal of Pharmaceutical Sciences and Research. 2010;1(9):88-96.
Subramanian SP, Saratha V. Evaluation of antibacterial activity of Calotropis gigantea latex extract on selected pathogenic bacteria. J Pharm Res. 2010;3(4):32-45.
Pradeepa S, Subramanian S, Kaviyarasan V. Evaluation of antimicrobial activity of Pithecellobium dulce pod pulp extract. Asian Journal of Pharmaceutical and Clinical Research. 2014;7(1):32-7.
Kirtikar KR, Basu BD. Indian medicinal plants. Indian Medicinal Plants; 1935.
Bhishagratna KK. An English translation of Sushruta Samhita based on the original Sanskrit text (Chowkhamba Sanskrit Series Office,Varanasi, India); 1991.
Kirtikar KR, Basu ED. Indian medicinal plants. (Ed) L.M. Basu, Allahabad. 1933; 1647.
Asima C, Satyesh CP. The treatise on Indian medicinal plants. Publications and Infornlation Directorate, CSIR. 2001;85-87.
Sanghi R, Bhattacharya B, Dixit A, Singh V. Ipomoea dasysperma seed gum: An effective natural coagulant for the decolorization of textile dye solutions. Journal of Environmental Management. 2006;81(1):36-41.
Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns. 1994;20:426-429.
Lall N, Meyer JJ. Antibacterial activity of water and acetone extracts of the root of Euclea natalensis. J Ethnopharmacol. 2000;72:313-316.
Wikler MA. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard. 8th edition. Document M07-A8, Pacific Beach Biosciences Inc.; 2008.
Lewis K, Ausubel FM. Prospects for plant-derived antibacterials. Nat Biotechnol. 2006;24(12):1504-7.
Shah PM. The need for new therapeutic agents: what is the pipeline? ClinMicrobiol Infect. 2005 11 (Suppl 3):36-42.
Shokeen P, Bala M, Tandon V. Evaluation of the activity of 16 medicinal plants against Neisseria gonorrhoeae. Int J Antimicrob Agents0 2009;33(1):86-91.
Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep. 2000;17(3):215-34.
Quave CL, Plano LR, Pantuso T, Bennett BC. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol. 2008;118(3): 418-28.
Martínez JL, Baquero F. Interactions among strategies associated with bacterial infection: Pathogenicity, epidemicity, and antibiotic resistance. Clin Microbiol Rev. 2002;15(4):647-79.
Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology. 2000;31:247-256
Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001;48(1):5-16.
Portillo A, Vila R, Freixa B, Adzet T, Cañigueral S. Antifungal activity of Paraguayan plants used in traditional medicine. J Ethnopharmacol. 2001;76(1): 93-98.
Brull S, Coote P. Preservative agents in foods: Mode of action and microbial resistance mechanisms. Int J Food Microbiol. 1999;50(1-2):1-17.
Packialakshmi N, Suganya C, Guru V.Antibacterial activity and green synthesis of silver nanoparticles using Strychnos potatorum seed and bark extract. Asian Journal of Phytomedicine and Clinical Research. 2014;2(3):127–138.
Gangwar U, Chowbey A. Asian Journal of Pharmaceutical Education and Research. 2017;6(1).
Alwe JR, Alwe RS. Harmacognostic Study of Strychnos potatorum Linn-A Review. International Ayurvedic Medical Journal. 2016;4(07).
Thavaranjit AC. In vitro antibacerial activity and phytochemical screening of Strychnos potatorum seed extract. Der Pharma Chemica. 2016;8:218-21.
Sharmila C, Renuka K, Subramanian SP. Biochemical evaluation of antidiabetic properties of strychnos potatorum seeds extract studied in high fat diet fed-low dose streptozotocin induced experimental type 2 diabetes in Rats. Research Journal of Pharmacy and Technology. 2020;13(6): 2615-2623.
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