Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy

Main Article Content

Mansour Abdulnabi H. Mehdi
Abdul-hakim M. A. Al- Alawi
Ahmed Zain A. Thabet
Fadel Y. S. Alarabi
Gozif Mohammed N. Omar
Vidya Pradhan

Abstract

Tamarindus indica is one of the medicinal plants used in the treatment of various diseases traditionally.

Aims: This study was conducted to identify the phytochemical constituents of T. indica leaf extracts. Methods: Using Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) to identification of bioactive compounds in extracts of T. indica.

Results: The FT-IR spectrum confirmed the presence of alcohol group, alkene group, amine group, carbonates, ethers, carboxylic acid and disulfides in both extracts.

A total of 22 and 38 bioactive phytochemical compounds were identified in the ethanolic and aqueous extracts of T. indica, respectively. The major bioactive compounds of the ethanolic extract of T. indica leaves were cis-Vaccenic acid, trans-13-Octadecenoic acid, Oleic Acid, Octadecanoic acid, Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester, Eicosanoic acid and Eicosane, 1-Iodo-2-methylundecane, 10-Methylnonadecane. While the major bioactive compounds of the aqueous extract were 3-O-Methyl-d-glucose, Myo-Inositol, 4C-methyl-, Myo-Inositol, 2-C-methyl-, Propane, 2,2-dimethoxy-, 1,3-Dioxolane, Ethanol, 2-(1-methylethoxy)-, and 2-Pentanone, 4-hydroxy-4-methyl-, 2-Hexanol, 2-methyl-, Ethanamine, N-methyl.

Keywords:
Tamarindus indica, bioactive chemical compounds, FT-IR, GC-MS.

Article Details

How to Cite
Mehdi, M. A. H., Alawi, A.- hakim M. A. A.-, Thabet, A. Z. A., Alarabi, F. Y. S., Omar, G. M. N., & Pradhan, V. (2021). Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy. Asian Journal of Research in Biochemistry, 8(1), 22-34. https://doi.org/10.9734/ajrb/2021/v8i130171
Section
Original Research Article

References

Kirbag S, Zengin F, Kursat M. Antimicrobial activities of extracts of some plants. Pak J Bot. 2009;41(4):2067-2070.

Koduru S, Grierson DS, Afolayan AJ. Ethnobotanical information of medicinal plants used for treatment of cancer in the Eastern Cape Province, South Africa. Curr Sci. 2007;906-908.‏

Sahayaraj PA, Gowri J, Dharmalingam V, Shobana R, Prema AA. Phytochemical screening by FTIR spectroscopic analysis of leaf and stem extracts of Wedelia biflora. Int J Nano Corros Sci Eng. 2015; 2(5):322-334.

Nithyadevi J, Sivakumar R. Phytochemical screening and GC–MS, FT-IR analysis of methanolic extract leaves of Solanum torvum sw. Int J Res Stud Biosci. 2015; 3(9):61-66.‏

Christenhusz MJ, Byng JW. The number of known plants species in the world and its annual increase. Phytotaxa. 2016;261(3): 201-217.‏

Wink M. Evolution of secondary metabolites in legumes (Fabaceae). Sou Afr J Bot. 2013;89:164-175.‏

Bhadoriya SS, Ganeshpurkar A, Narwaria J, Rai G, Jain AP. Tamarindus indica: Extent of explored potential. Pharma Rev. 2011;5(9):73–81.

Mabberley DJ. Mabberley's plant-book: A portable dictionary of plants, their classifications and uses (No. Ed.3). Cambridge University Press; 2008.‏

Zohrameena S, Mujahid M, Bagga P, Khalid M, Noorul H, Nesar A, Saba P. Medicinal uses & pharmacological activity of Tamarindus indica. World J Pharm Sci. 2017;5(2):121-133.

Gupta R. Investigation of antidiarrhoeal activity of ethanolic extract of Tamarindus indica L. seeds in albino Wistar rats. Asian J Pharm. 2016;10(04):492-496.

Mehdi MAH, Pradhan V, Salem KNQ, Omar GMN, Alarabi FY. Effect of ethanol extract of Pancratium maximum bulbs on Entamoeba histolytica in vivo. European Journal of Biomedical. 2020;7(12):317-322.‏

Mehdi MAH, Alarabi FY, Farooqui M, Pradhan V. Phytochemical screening and antiamebic studies of Tamarindus indica of leaves extract. Asian J Pharm Clin Res. 2019;12(2):507-512.‏ Available:http://dx.doi.org/10.22159/ajpcr.2019.v12i2.29684

Mehdi MAH, Alarabi FY, Omar GMN, Salem KNQ, Pradhan V. Evaluation of haematological changes in Entamoeba histolytica-infected rats administered with extracts of Ziziphus mauritiana leaves. Research Journal of Pharmacy and Technology. 2020;13(9):4105-4110.‏ Available:https://doi:10.5958/0974-360X. 2020.00725.8

Al-Arabi FY, Omar GM, Mehdi MA, Pradhan V.The effect of Tamarindus indica extracts on larval stage of Echinococcus granulosus parasite In vivo and In vitro study.‏ Pharma Innov J. 2018;7(11):22-26.

Satpute S, Vanmare DJ. In vitro antifungal activity of Tamarindus indica L. extract against pathogenic fungi. International Journal of Botany Studies. 2017;2(3):25-28.

Al-Ahdab MA. Anti-Hyperglycemic effect of Tamarindus indica extract in Streptozotocin-induced diabetes in male rats. World Appl Sci J. 2015;33(12):1940-1948.

Harborne J. Phytochemical methods, a guide to modern techniques of plant analysis. Springer Science & Business Media; 1998.

Mishra J, Tewari S, Singh S, Arora NK. Biopesticides: Where we stand? In Plant microbes symbiosis: Applied facets. Springer, New Delhi.‏ 2015;37-75.

Antony TS, Peter MJ, Raj JY. Phytochemical Analysis of Stylosanthes fruticosa using UV-VIS, FT-IR and GC-MS. Research Journal of Chemical Sciences. 2013;3(11):14-23.‏

Nithyadevi J, Sivakumar R. Phytochemical screening and GC–MS, FT-IR analysis of methanolic extract leaves of Solanum torvum sw. International Journal of Research Studies in Biosciences. 2015; 3(9):61-66.

Mehdi MAH, Alarabi FY, Omar GMN, Pradhan V. Effect of extracts on haematological parameters in albino rats Tamarindus indica infected with parasite Entamoeba histolytica. Asian Journal of Pharmacy and Pharmacology. 2019;5(5) :889-894.‏ Availabe:https://doi.org/10.31024/ajpp.2019.5.5.5

Mehdi MAH, Omar GMN, Farooqui M, Pradhan V. Therapeutic effect of Tamarindus indica extracts on the pathogenesis of Entamoeba histolytica In-vivo. International Journal of Pharmaceutical Sciences and Research. 2019;10(7):3250-3256. Availabe:http://dx.doi.org/10.13040/IJPSR.0975-8232.10(7).3250-56

Mehdi MAH, Pradhan V. The antiamoebic potential of aqueous extract of Pancratium maximum bulbs in rats infected with Entamoeba histolytica.‏ Mukt Shabd Journal. 2020;9(6):457-466.

Correa SN, Naranjo AM, Herrera AP. Biosynthesis and characterization of gold nanoparticles using extracts of Tamarindus indica L leaves. J Phys. 2016;687(1): 012082.

Pérez B, Teixeira C, Albuquerque IS, Gut J, Rosenthal PJ, Prudêncio M. Gomes P. PRIMACINS, N-cinnamoyl-primaquine conjugates, with improved liver-stage antimalarial activity. Med Chem Comm. 2012;3(9):1170-1172.‏

Brazhko OО, Zavgorodny MP, Kruglyak OS, Omeljanchik LO, Shapoval GA. Antioxidant activity of alkoxy derivatives of (quinolinе-4-ylthio) carboxylic acids. Ukr Biochem J. 2015; 87(2):95-102.‏

Phuong TV, Lam V. Diep CN. Bioactive compounds from Marine streptomyces Sp. by Gas Chromatography-Mass Spectrometry. Pharm Chem J. 2018;5(1): 196-203.

Hussein JH, Ubaid JM, Hameed IH. Gas chromatography–mass spectrum analysis of volatile components of methanolic leaves extract of Cordia myxa. Int J Curr Pharm Rev Res. 2017;7(6):16-22.‏

Arora S, Kumar G. Phytochemical screening of root, stem and leaves of Cenchrus biflorus Roxb. J Pharmacog Phytochem. 2018;7(1):1445-1450.‏

Abdullah BM, Mehdi MAH, Khan AR, Pathan JM. Gas chromatography-mass spectrometry (GC-MS) Analysis of Ajwain (Trachyspermum ammi) Seed Extract.‏ International Journal of Pharmaceutical Quality Assurance. 2020;11(2):228-231. Availabe:https://doi.org/10.25258/ijpqa.11.2.6

Godara P, Dulara BK, Barwer N, Chaudhary NS. Comparative GC-MS analysis of bioactive phytochemicals from different plant parts and callus of Leptadenia reticulata wight and arn. Pharmacog J. 2019;11(1):129-140.‏

George LO, Radha HR, Somasekariah BV. In vitro anti-diabetic activity and GC-MS analysis of bioactive compounds present in the methanol extract of Kalanchoe pinnata.‏ Indian J Chem. 2018; 1213-1221.

Shareef HK, Muhammed HJ, Hussein HM, Hameed IH. Antibacterial effect of ginger (Zingiber officinale) roscoe and bioactive chemical analysis using gas chromatography mass spectrum. Orient J Chem. 2016;32(2):20-40.‏

Hussein HM, Hameed IH, Ibraheem OA. Antimicrobial Activity and spectral chemical analysis of methanolic leaves extract of Adiantum capillus-veneris using GC-MS and FT-IR spectroscopy. Int J Pharmacog Phytochem Res. 2016;8(3): 369-385.‏

Patel J, Reddy V, Kumar G, Satyasai D. Bajari B. Gas chromatography and mass spectroscopy analysis of bioactive components on the leaf extract of Terminalia coriacea: A potential folklore medicinal plant. Int J Green Pharm. 2017; 11(01):143‏.

Yakubu EO, Otitoju O, Onwuka J. Gas chromatography- mass spectrometry (GC-MS) analysis of aqueous extract of Daniellia oliveri stem bark. Pharm Anal Acta. 2017; 8(568):1-8.