Antioxidant Potentials, Phytochemical Profiles and Ameliorating Effects of Senna siamea Extract on Arsenic-Induced Hepato-Renal Damage
Asian Journal of Research in Biochemistry,
This study evaluated the in- vitro antioxidant, phytochemical constituents and hepato-renal protective efficacy of Senna siamea methanolic extract in arsenic-induced oxidative stress. The results of this study show that S. siamea has strong antioxidant potentials against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’azinobis (3 ethylbenzothiazoline-6-sulphonic acids) (ABTS) radicals. The extract scavenges nitric oxide radicals and has strong ferric reducing power. It also inhibited the induction of lipid peroxidation and α-amylase activity in a concentration-dependent manner. Administration of arsenic to rats induced a significant increase in the levels of alanine aminotransferase, aspartate aminotransferase, cholesterol, urea, creatinine, and triglycerides in the plasma, while it decreased superoxide dismutase, glutathione and catalase activities in the liver and kidney. It also significantly reduced the levels of white blood cells, red blood cells, platelet and lymphocytes in the blood. However, rats pretreated with S. siamea extract before arsenic administration significantly reversed all these arsenic-induced changes. Histological observations showed varying degrees of liver damage in the arsenic group that was untreated, while the pre-treatment with S. siamea extract significantly improved the general histoarchitecture of the liver. The phytochemical constituents of the extract were determined, the gas-chromatography analysis revealed the possible presence of oxalic acid, barakol, kaempferol, betulin while total phenolics content, crude protein, crude fat, crude fibre are in considerable amount in the plant This study demonstrated that S. siamea extract has antioxidant potentials and ameliorates arsenic-induced hepato-renal toxicity, the effectiveness of S. siamea as a medicinal plant could be due to the presence of various phenolics and antioxidant compounds in the plant.
- Senna siamea
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Lin CC, Huang PC. Antioxidant and hepatoprotective effects of Acathopanax senticosus. Phytother. Res. 2002;14:489–494.
Veeresham C. Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res. 2012;3(4):200– 201.
Lee HJ, Hyun EA, Yoon WJ, Kim BH, Rhee MH, Kang HK, Cho JY, Yoo ES. In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacol. 2006;103: 208–216.
Afsane B, Thozhukat S, Seyed AM, Amirhossein S. Counteracting arsenic toxicity: Curcumin to the rescue? Journal of Hazardous Materials. 2020;400:123160.
Mennen LI, Sapinho D, Bree AD, Arnault N, Bertrais S, Galan P, Hercberg S. Consumption of Foods Rich in Flavonoids Is Related to a Decreased Cardiovascular Risk in Apparently Healthy French Women. Journal of Nutrition. 2004;134:923-926.
Sati SH, Rawat U, Sati OP. Medicinal plants as a source of antioxidants. Research Journal of Phytochemistry. 2010;4:13-224.
Kupradinun P, Tepsuwan A. Tantasi N, Meesiripun N, Rungsipipat A, Kusamran WR. Anticlastogenic and anticarcinogenic potential of Thai bitter: Gourd fruits. Asian Pacific Journal of Cancer Prevention. 2011;12(5):1299-1305.
Deguchi J, Hirahara T, Hirasawa Y, Ekasari W, Widyawaruyanti A, Shirota O, Shiro M, Morita H. New tricyclic alkaloids, cassiarins G, H, J, and K from leaves of Cassia siamea. Chem Pharm Bull (Tokyo). 2012;60(2):219-22.
Owoade AO, Alausa AO, Adetutu A, Olorunnisola OS, Owoade, AW. Phytochemical Characterization and Antioxidant Bioactivity of Andrographis paniculata (Nees). Pan African Journal of Life Sciences. 2021:5(2):246-256
Schlesier K, Harwat M, Böhm V, Bitsch R. Assessment of Antioxidant Activity by Using Different in vitro Methods. Free Radical Research. 2002;36:177-187.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cationdecolorization assay. Free Radical Biology and Medicine. 1999;26(9–10):1231–1237.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine, Japan Journal Nutrition. 1986;44:307-315.
Garrat DC. The Quantitative analysis of Drugs. Chapman and Hall Ltd., Japan. 1964;3:456-458
Janero DR. “Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury,” Free Radical Biology and Medicine. 1990;9(6):515–540
Bernfeld P. Amylases, Alpha and Beta, Vol. 1, Academic Press, NY.1995;149.
Miliauskas G, Venskutonis P, Beek T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004;85:231-237.
Sofowara A. Medicinal Plants and Traditional Medicine in Africa, Spectrum Books, Ibadan, Nigeria. 1993;289.
Ejikeme CM, Ezeonu CS, Eboatu AN. “Determination of physical and phytochemical constituents of some tropical timbers indigenous to Niger Delta Area of Nigeria,” European Scientific Journal. 2014;10(18):247-270.
Santhi K, Sengottuvel R. Qualitative and quantitative phytochemical analysis of Moringa concanensis Nimmo. Int.J.Curr.Microbiol.App.Sci. 2016;5(1):633-640.
Harborne JB. Phytochemical methods. London: Chapman and Hall, Ltd. 1973;49-188.
Ajiboye BO, Ibukun EO, Edobor G, Ojo AO, Onikanni, SA. Int. J. Inv. Pharm. Sci. 2013;1(5):428-432
Udo EJ, Oguwele JA. Laboratory manual for the analysis of soil, plants and water samples. 3rdedition, department of crop production, University of Ilorin, Kwara State Nigeria. 1986;131 –152.
James, C. S. Analytical chemistry of food. Chapman and Hall, London. 1995;64– 65.
AOAC. Official Methods of Analysis. 14thedition, Association of Official Analytical Chemists, Washington DC;1990.
Isong, E, Idiong. Nutrient content of the edible leaves of seven wild plants from Nigerian. Plant foods for human nutrition. 1997;51:79-84.
Jollow DJ, Michell JR, Zampaglione N, Gillete, J. Bromobenzene- induced liver necrosis. Protective role of glutathione an evidence for 3,4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacology. 1974;11:151-169.
Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 1972;247:3170-3175.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods. Enzymol. 1978;52: 302-310.
Sinha KA. Colorimetric assay of catalase. Anal Biochem. 1971;47:389-394.
Owoade AO, Adetutu A, Olorunnisola OS, Ayinde KS. The in- vitro Antioxidant Properties and Phytochemical Constituents of Citrullus Colocynthis Methanolic Extract. Elixir Appl. Botany. 2018;121:51556-51562.
Owoade OA, Aborishade AB, Adetutu A, Olorunisola OS. Evaluation of the Effectiveness of Telfairia occidentalis Leave Extracts in the Amelioration of Carbon Tetrachloride Induced Liver Injuries and Oxidative Damage in Rats. Journal of Advances in Medical and Pharmaceutical Sciences. 2016;10(2):1-11.
Adedoyin B, Muhammad A, Dangoggo SM, Rabah A, Sharples G, Nahar L, Sarker S. Chemical Composition and Bioactivity of the Essential Oil of Cassia singueana Flowers Growing in Nigeria. Pharm Biomed Res. 2019;5(3):1-7
Coppen IN, Allen JC, Hamilton RJ. Rancidity in foods (Applied Science Publishers, New York, USA 1983.
Bailey CJ. New Approaches to the Pharmacotherapy of Diabetes, Vol. 2, 3rd Edition, Blackwell Science Ltd., UK. 2003;73.1-73.21.
Hanamura T, Hagiwara T, Kawagishi H. Structural and functional characterization of polyphenols isolated from acerola (Malpighia emarginata DC.) fruit. Biosci Biotechnol Biochem. 2003;69:280- 286.
Rajesh MG, Latha MS. Preliminary evaluation of the antihepatotoxic effect of Kamilari, polyherbal formulation. Journal of Ethnopharmacology. 2004;91:99-104
El-Wessemy AM. Histopathological and ultra-structural studies on the side effects of the analgesic drug tramadol on the liver of albinomice. Egypt. J Zool. 2008;50:423-442.
Battacharya R, Shivanandappa T. Antioxidant activity and hepatoprotective potential of triplochiton scleroxylon k. schum. (sterculiaceae). International Journal of Applied Biology and Pharmaceutical Technology. 2003;3(2):68-76
Ichikawa T, Liang J, Kitajima S, Koike T, Wang X, Sun H, et al. Macrophage-derived lipoprotein lipase increases aortic atherosclerosis in cholesterol-fed Tg rabbits. Atherosclerosis. 2005;179(1):87– 95.
Folorunsho AA, Olorunnisola OS, Adetutu A, Owoade AO. Prolong Administration of Methanolic Whole Fruit Extract of Lagenaria breviflora (Benth.) Roberty Provoke Oxidative Stress and Kidney Dysfunction in Male Wistar Rats. J Bioanal Biomed. 2019;11:142-148
Esievo KB., Fatokun, OT, Ibrahim JA, Kunle OF. Pharmacognostic Studies of the Leaf of Senna siamea (lam.) Irwin & Barneby Family: Caesalpiniaceae. European Journal of Medicinal Plants. 2016;16(2):1-7.
Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1)44–84.
Ademola AO, Temidayo OO, Ebunoluwa RA, John OA, Adeolu AA, Momoh AY. Kolaviron attenuated arsenic acid induced-cardiorenal dysfunction via regulation of ROS, C-reactive proteins (CRP), cardiac troponin I (CTnI) and BCL2. Journal of Traditional and Complementary Medicine. 2018;8:396-409.
Soetan KO, Akinrinde AS, Ajibade TO. Preliminary studies on the haematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor). Proceedings of 38th Annual Conference of Nigerian Society for Animal Production. 2013;49-52.
Roa RR, Babu RM, Rao MRV. Saponins as anti-carcinogens. The J. Nutr. 1995;125:717-724.
Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouring Agents. Global J. Pure Appl. Sci. 2001;7(3):455-459.
Trease GE, Evans WC. Phytochemicals. In: Pharmacognosy. 15th ed. Saunders Publishers, London. 2002;42:221-229,
Thongsaard W, Pongsakorn S, Sudsuang R, Bennett GW, Kendall DA, Marsden CA. "Barakol, a natural anxiolytic, inhibits striatal dopamine release but off uptake in vitro". European Journal of Pharmacology. 1997;319(2–3):157–164.
Sukma M, Chaichantipyuth C, Murakami Y, Tohda M, Matsumoto K, Watanabe H. "CNS inhibitory effects of barakol, a constituent of Cassia siemia Lamk". Journal of Ethnopharmacology. 2002;83(1–2):87–89
Hongsirinirachorn M, Threeprasertsuk S, Chutaputti A. "Acute hepatitis associated with Barakol". Journal of the Medical Association of Thailand = Chotmaihet Thangphaet. 2003;86(2):S484-489.
Arnon SS, Schechter R, Inglesby TV, Henderson DA, Bartlett JG, Ascher MS, Eitzen E, Fine AD, Hauer J, Layton M, Lillibridge S, Osterholm MT, O'Toole T, Parker G, Perl TM, Russell PK, Swerdlow DL, Tonat K. "Botulin as a biological weapon: medical and public health management". JAMA. 2001;285(8):1059–70
Adeyeye EJ, Ayejuyo OO. Chemical composition of Cola acuminates and Garcina kola seed grown in Nigeria. International Journal of Food Science and Nutrition. 1994;45:233-223.
Ishida H, Suzuno H, Sugiyama N, Innami S, Todokoro T, Maekawa A. Nutritional evaluationof chemical component of leaves and stems of sweet potatoes (Euphorbiaceae). African Journal of Biochemistry. 2000;6(24):2752-2757.
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