Protective Effect of Ipomoea biloba on Myocardial Antioxidant Status in Isoproterenol Induced Myocardial Infarction Male Albino Rats
Asian Journal of Research in Biochemistry,
The protective effect of ethanolic leaf extract of Ipomoea biloba in isoproterenol (ISO)-induced cardiotoxicity and the antioxidant activity involved in this protection were investigated in rats. Myocardial infraction was produced in rats with 20 mg/kg b.wt of ISO administered subcutaneously twice at an interval of 24 h. Effect of EEIB oral treatment for 28 days at two doses (100 mg and 200 mg/kg body weight) was evaluated against ISO – induced cardiac necrosis. Level of enzymatic (SOD, CAT, GPx and GST), non-enzymatic (GSH, Vitamin C and E) and of membrane bound ATPases (Na+K+ATPase, Mg2+ATPase and Ca2+ATPase) were assayed in heart homogenate. Significant myocardial infarction, depletion of endogenous antioxidants enzymatic and non-enzymatic were observed in ISO-treated animals when compared with the normal animals. Rats induced with ISO, showed a significant (P<0.05), decrease in the activities of GSH, Vitamin C and Eon comparison with normal rats. EEIB elicited a significant cardioprotective activity by elevated the levels of GSH, SOD, CAT, GPx and GR. A significant decrease in the activity of Na+/K+ ATPase and a corresponding increase in the activities of Ca2+ ATPase and Mg2+ ATPase were observed in isoproterenol induced rats when compared to normal control rats. Pretreatment with EEIB was able to efficiently prevent the increase in activity of Mg2+ ATPase and maintain the activities of Na+ /K+ ATPase and Ca2+ ATPase at near normality. There is no significant difference between the control and plant alone treated rats. The aim of this investigation is to evaluate the antioxidant effects on the main cardioprotective activity of ethanolic leaf extract Ipomoea biloba.
- Ipomoea biloba
- myocardial infarction.
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Tracey Roizman DC. What Are the Benefits of Eating Antioxidants?;2018.
Kandhan Karthishwaran, Salem Obaid Saeed Obaid Al Shamisi, Shyam Sreedhara Kurup, Sabitha Sakkir & Abdul Jaleel CheruthFree-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities and in vitro studies in Caralluma flava N. E. Br. Pharmaceutical Biotechnology. 2017;156-162.
Poljsak B, Milisav I. The Neglected Significance of Antioxidative Stress. Oxidative Medicine and Cellular Longevity. 2012;480895.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions. 2006;160(1):1–40.
Arika W, Kibiti CM, Njagi JM, Ngugi MP. In vitro antioxidant properties of dichloromethanolic leaf extract of Gnidia glauca (Fresen) as a promising antiobesity drug. Journal of Evidence-Based Integrative Medicine. 2019;24.
Sinha AK. Colorimetric assay of catalase. Analytical biochemistry. 1972;47(2):389-394.
Habig WH, Jakoby WB. Assays for differentiation of glutathione S-Transferases. In Methods in enzymology. 1981;77:398-405.
Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. In Methods in enzymology. 1979;62:3-11.
Anand A. Zanwar, Mahabaleshwar V. Hegde, Subhash L. Bodhankar Cardioprotective activity of flax lignan concentrate extracted from seeds of Linum usitatissimum in isoprenalin induced myocardial necrosis in rats. 2011;4(2): 90–97.
Yoshida T, Watanabe M, Engelman DT, Engelman RN, Schley JA, Maulik N, Ho YS, Oberley TD, Das DK. Transgenic mice overexpressing glutathione peroxidase are resistant to myocardial ischemia reperfusion injury. J Mol Cell Cardiol. 1996;28:1759-1767.
Leung HW, Vang MJ, Mavis RD. The co-operative interaction between Vit-E and Vit-C suppression of peroxidation of membrane phospholipids. Biochem Biophys Acta. 1981;664:266-272.
Middleton E Jr. The flavonoids. Trends pharmacol Sci. 1984;5:335-338.
Tirmenstein MA and Reed DJ Characterization of glutathione-dependent inhibition of lipid peroxidation of isolated rat liver nuclei. Arch Biochem Biophys. 1988;261: 1-11.
Fernandez-Checa JC, Garcia-Ruiz C, Ookhtens M and Kaplowitz N Impaired uptake of glutathionine by hepatic mitochondria from chronic ethanol-fed rats. Tracer kinetic studies invitro and susceptibility to oxidant strews. J Clin Invest. 1991;87:397-405.
Meister A. Glutathione deficiency produced by inhibition of its synthesis, and its reversal, applications in research and therapy. Pharmacal Ther. 1991;51:155-194.
Martensson J, Lai JCK. Meister A High affinity transport of glutathione is part of a multi component system, essential for mitochondrial function. Proc Natl Acad Sci USA. 1990;87:7185-7189.
Polidoro G, De Ilio C, Aruduini A, La Rovere G, Federici G. Supperoxide dismutase reduced glutathione and TBA-reactive products in erythrocytes of patients with multiple sclerosis. Int J Biochem. 1984;16: 505-509.
Litov RE, Mathew CL and Tappel AL Vitamin E protection against in vivo lipid peroxidation initiated in rats by methyl ethyl ketone peroxide as monitored by pentane. Toxicol Appl Pharmacol.1981;58:96- 106.
Condell RA, Tappel AL. Evidence for suitability of glutathione peroxidase as a protective enzyme: studies of oxidative damage, renaturation, and proteolysis. Arch Biochem Biophy. 1983;223:407-416.
Meister A. New aspect of glutathione biochemistry and transport selective alterations of glutathione metabolism Nutr Rev. 1984;42:397-400.
Vandana S Panda, Suresh R Naik. Naik. Cardioprotective activity of Ginkgo biloba Phytosomes in isoproterenol-induced myocardial necrosis in rats: A biochemical and histoarchitectural evaluation Experimental and Toxicologic Pathology. 2008;60:397-404.
Sharma M, Kishore K, Gupta SK, Joshi S, Arya DS. Cardioprotective potential of Ocimum sanctum in isoproterenol induced myocardial infraction in rats. Mol Cell Biochem. 2001;225:75-83.
Marikannan M, Darlin Quine S. Ellagic acid protects mitochondria from β-adrenergic agonist induced myocardial damage in rats; evidence from in vivo, in vitro and ultra-structural study. Food Res Int. 2012;45:1-8.
Ganapathy P, Rajadurai M Cardioprotective effect of β-sitosterol on isoproterenol induced myocardial infarction in male albino wistar rats. Periyar University Thesis;2015.
Jayalakshmi R, Niranjali Devaraj S. Cardioprotective effect of tincture of Crataegus (TCR) on experimentally induced myocardial infraction in rats University of madras Shodhganga @INFLIBNET: Cardioprotective effect of tincture of crataegus TCR on experimentally induced myocardial infarction in rat;2005.
Paritha IA, Devi CS. Effect of α- tocopherol on lipid peroxidation in isoproterenol induced myocardial infraction in rats. Indian J. Biochem Biophys. 1997;41:369-376.
Rajadurai M. Stanely Mainzen Prince Preventive effect of naringin on isoproterenol – induced cardiotoxicity in Wistar rats: an in vivo and in vitro study Toxicology. 2007;232.
Mourelle M, Franco MT. Erythrocyte defects procedure the onset of CCl4 induced liver cirrhosis protection by silymarin. Life Sci. 1981;48:1083-1090.
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