Phytochemical Screening and In-vitro Antioxidant Activity of the Aqueous Extract of Detarium Senegalense Root Bark
Asian Journal of Research in Biochemistry,
Aims: To screen for the presence of bioactive antioxidant phytochemicals and determine the antioxidant activity of Detarium Senegalense root bark.
Place and Duration of Study: Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Nigeria, between April 2018 and December 2018.
Methodology: Two solvents, water mixed with chloroform (70:30) was used for extraction. The plant extract was screened for the presence of phytochemicals by standard qualitative analysis and evaluated for in vitro antioxidant activity by determining the reducing power, total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and nitric oxide radical scavenging activity in comparison with ascorbic acid and gallic acid.
Results: The reducing power and nitric oxide scavenging activity of the extract increased in a concentration/dose dependent manner and was significantly (P<.05) lower when compared to ascorbic acid and gallic acid at all concentrations tested. The total antioxidant capacity (TAC) of the extract also increased as the concentration increased. Interestingly, at 1000µg/ml, the extract (201.45±0.95) was found to be significantly higher (p<.05) than that of ascorbic acid (198.36±0.83), although lower than gallic acid (266.50±0.84). The % DPPH inhibition of the extract was also significantly lower when compared to ascorbic acid and gallic acid at all concenterations tested. Overall, the results showed the extract was able to scavenge free radicals in a dose dependant manner and revealed the presence of tannins, steroids, alkaloids, terpenoids, saponins and phenols whose synergistic effect may be responsible for the antioxidant activity of the extract.
Conclusion: From the study, it is concluded that the aqueous extract of Detarium Senegalense root bark possess appreciable/considerable antioxidant properties and could be exploited as source of antioxidant additives or supplements. However, there is need for further work to clarify and isolate the different classes of phytochemical constituents and also to investigate it’s in vivo potential.
- Antioxidant activity
- aqueous extract
- phytochemical constituents
- Detarium Senegalense
How to Cite
Pisoschi AM, Pop A, Iordache F, Stanca L, Predoi G, Serban AI. Oxidative stress mitigation by antioxidants-an overview on their chemistry and influences on health status. European Journal of Medicinal Chemistry. 2021 Jan 1;209:112891.
Davies, K.J.A. Oxidative Stress, Antioxidant Defenses, and Damage Removal, Repair, and Replacement Systems. 2000;50:279-289. IUBMB Life.
Liu RH. Potential synergy of phytochemicals in cancer prevention. J Nutr. 2004;134:3479S.
Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003; 78(suppl):517S
Hertog MGL, Feskens EJM, Hollman PCH, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen elderly study. Lancet. 1993;342:1007.
Knect P, Jarvinen R, Reunanen A, Maatela J. Activity of Artichoke Leaf Extract On Reactive Oxygen Species In Human Leukocytes. Br Med J. 1996;312:478.
Arai Y, Watanabe S, Kimura M, Shimoi K, Mochizuki R, Kinae N. Dietary intakes of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol concentration. The Journal of Nutrition. 2000;130:2243– 2250.
Block G, Patterson B, Subar A. Fruit, vegetables, and cancer prevention: A review of the epidemiological evidence. Nutr Cancer. 1992;18:1.
Uchegbu IR, Okwu ED. An Evaluation of the Phytochemical and Nutrient Composition of the Seeds and Stem Bark of Detarium senegalense Gmelin. Journal of Natural Sciences Research, 2012;2(5).
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online).
Wang Q, Ellis PR, Ross-Murphy SB, Burchard W. Solution characteristics of the Xyloglucan extracted from Detarium senegalense Gmelin. Carbohydrate Polymers. 1997;31:115-124
Burkill,H.M. The useful plants of West Tropical Africa. (vol.3) Royal Botanic Gardens, London. 1995;101.
Sowemimo, AA, Pendota G, Okoh B. Omotosho T, Idika N, Adekunle AA, and Afoloyan AJ. Chemical composition, antimicrobial activity, proximate analysis and mineral contents of the seed of Detarium senegalense JF Gmelin. Afri. J. Biotech. 2011;10(48):9875-9878.
Harbone J.B. The Flavonoids: Advances in Research since 1980. Chapman and Hall, London; 1988.
Evans W, Fauci A. Trease and evans pharmacognosy, New and reemerging diseases: The importance of biomedical research. Emerging Infectious Diseases. WB Saunders Company Ltd., London; 1998.
Siddiqui AA, Ali M. Practical pharmaceutical chemistry, 1st edn, CBS Publishers and distributors, New Delhi. 1997;126:1317.
Aliyu AB, Ibrahim MA, Musa AM, Ibrahim H, Abdulkadir IE, Oyewale AO. Evaluation of antioxidant activity of leave extract of Bauhinia rufescens Lam. (Caesalpiniaceae). Journal of Medicinal Plants Research. 2009;3(8):563-567.
Available:http://www.academicjournals.org/JMPR ISSN 1996-0875© 2009 Academic Journals
Prieto P, Pineda M, Aguilar M. Spectophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 2005;269:337–341. [PubMed] [Google Scholar]
Sun H, Wang ZB. Effects on exercise endurance capacity and antioxidant properties of astragalus membranaceus polysaccharides (APS). J. Med. Plants Res. 2010;4:982-986.
Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Jpn J. Nutrition. 1986;44:307- 315.
Ozdestan, O. and Uren, A. Development of a cost-effective method for nitrate and nitrite determination in leafy plants and nitrate and nitrite contents of some green leafy vegetables grown in the Agean region of Turkey. Journal of Agricultural and Food Chemistry. 2010;58:5235-5240.
Vit K., Zuzuna R., Katerina K. and Lu O. Condensed and Hydrolysable Tannins as Antioxidants Influencing the Health. Mini Reviews in Medicinal Chemistry. 2008; 8(5):436-47.
Brindhadevi, K, Chidambaram, M, Kavitha, R. et al. Extraction, antioxidant, and anticancer activity of saponins extracted from Curcuma angustifolia. Appl Nanosci; 2022.
Okwu DE, Omodamiro OD. Effect of Hexane Extract and Phytochemical Content of Xylopia aethiopica and Ocimum gratissimum on uterus of guinea Pig. Bio-Research. 2005;3:40-44.
Okwu D. Evaluation of the chemical composition of medicinal plants belonging to Euphorbiaceae. Pakistan Vertirenary Journal. 2001;14:160-162.
Li X, Wu X, Huang l. Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui). Molecules. 2009;14:5349- 5361.
Sim KS, Sri Nurestri AM, Norhanom AW. Phenolics content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts. Pharmacog. Magazine. 2010; 6(23):248-254.
Kahkonen Mp, Hopia AI, vuorela HJ, rauha JP, Pihlaja K, Kulaja TS, Heinonen M. Antioxidant activity of plants extracts containing phenolics compounds. J. Agric. Food Chem. 1999;47:3954-3962.
De Oliveira VS, Ferreira FS, Cople MCR, Labre TDS, Augusta IM, Gamallo OD, Saldanha T. Use of Natural Antioxidants in the Inhibition of Cholesterol Oxidation: A Review. Compr. Rev. Food Sci. Food Saf. 2018;17:1465– 1483.
DOI: 10.1111/1541-4337.12386. [PubMed] [CrossRef] [Google Scholar]
Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K. Methods for testing antioxidant activity. Analyst. 2002;127(1):183–198.
Khan RA, Khan MR, Sahreen S, Ahmed M. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chemistry Central Journal. 2012;6:43.
Luo A, Fan Y and Luo A. In vitro free radicals scavenging activities of polysaccharide from Polygonum Multiflorum Thunb. Journal of Medicinal Plants Research. 2011;5(6):966-9 72.
Available:http://www.academicjournals.org/JMPR ISSN 1996-0875 ©2011 Academic Journals
Pracheta S., Ritu Paliwal. and Sadhana S. Preliminary Phytochemical Screening and in vitro Antioxidant Potential of Hydro-Ethanolicextract of Euphorbia neriifolia Linn. International Journal of PharmTech Research. 2011;3(1):124-32.
Shahid A. and Shoib A. Determination of total phenolic and flavonoids content, antimicrobial and antioxidant activity of root extract of Arisaemia jacquemonti Blume. Journal of Taibal University for Science. 2014;9:449-454.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nat. 1958;181:1199-1200.
Olayinka A. and Anthony O. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. 2010;10:21.
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