Asian Journal of Research in Biochemistry https://journalajrb.com/index.php/AJRB <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Asian Journal of Research in Biochemistry en-US Asian Journal of Research in Biochemistry 2582-0516 Extraction and Comparative Characterization of Oils from Edible Seeds of Glycine max and Sesamum indicum https://journalajrb.com/index.php/AJRB/article/view/30185 <p><strong>Aim:</strong> The incidence of heart-related disease conditions due to consumption of cholesterol containing oils and the increasing global demand for oil for domestic and industrial purposes have necessitated the need for scientific evaluation of other neglected indigenous plants for potential quality oil yield. This study thus aimed at extracting and comparing physicochemical and nutritional properties of seed oils from <em>Glycine</em> <em>max</em> and <em>Sesamum</em> <em>indicum</em> with a view to diversifying alternative sources of oil to meet teeming industrial and health needs and for food security.</p> <p><strong>Methodology:</strong> Solvent extraction method was employed to extract oil from seed samples which were further subjected to estimation of physicochemical indices such as free fatty acid, saponification value, iodine value, peroxide value, specific gravity, refractive index, density, pH, melting temperature and viscosity according to methods described by using titration method.</p> <p><strong>Results:</strong> Findings indicated higher saponification (412.33 mgKOH/mg), acid (2.99 mgKOH/g), free fatty acid (1.49 mgKOH/g), viscosity (0.13 Pas) and melting point (5.66<sup> o</sup>C) values for sesame seed oil than for soybean seed oil. However, soybean seed oil showed higher density (0.837 g/ml), specific gravity (0.842 Kg/m<sup>3</sup>), pH (6.21), iodine (47.25 wij’s or g/iodine/g) and peroxide (40.80 mEq/Kg) indices than that obtained for sesame seed oil though mean values were not significantly different.</p> <p><strong>Conclusion</strong>: From observed measured parameters, sesame seed may be suitable for industrial purposes such as the production of soap and paint owing to its high saponification, free fatty acid, viscosity and melting point values while soybean seed oil may be excellent source for domestic and nutritional consumption in addition to industrial use.</p> Victor Henry Azubuike Enemor Ejike Celestine Orji Uchechukwu Chibuzo Ogbodo Ogechukwu Frances Nworji Chinaza Lucy Ibeneme ##submission.copyrightStatement## 2021-06-22 2021-06-22 1 9 10.9734/ajrb/2021/v8i430185 Bioactive Composition and Acute Oral Toxicity Studies on Persea Americana Seed Ethyl Acetate Fraction https://journalajrb.com/index.php/AJRB/article/view/30186 <p><strong>Aims: </strong><em>Persea americana (P. americana) </em>dubbed ‘green gold’ is a highly sought after fruit today, with insatiable export market. Different parts of avocadoes have been consumed both for nutritional and health benefits across regions of the world. Therefore, this study investigates the bioactive composition of <em>P. americana </em>seed ethyl acetate fraction and acute toxicological effects.</p> <p><strong>Place and duration of study:</strong> Department of Biochemistry, Federal University of Technology Owerri, Imo State, Nigeria; between May 2019 and October, 2019.</p> <p><strong>Methodology:</strong> Quantitative phytochemical composition was assessed using gas chromatography fitted with flame ionization detector (GC-FID) and acute toxicity determined using standard method.</p> <p><strong>Results:</strong> Result of quantitative phytochemical composition of <em>P. americana </em>seed fraction shows a rich presence of phytochemicals such as epicatechin, kaempferol, proanthocyanin, rutin, resveratrol, ribalinidine, naringin, spartein, quinine, flavan-3-ol, anthocyanin, lunamarin, sapogenin, flavonones, flavones. The quantitative phytochemical composition of <em>P. americana </em>seed shows that among other phytochemicals, the seed is relatively rich in anthocyanin, quinine, epicatechin, tannin and proanthocyanin with concentrations of 69.39 ± 8.33 µg/g, 22.16 ±1.77 µg/g, 21.88 ± 2.53 µg/g, 19.86 ± 1.19 µg/g and 10.98 ± 0.55 µg/g respectively. The acute toxicity studies on the seed reveal that the ethyl acetate fraction of <em>P. americana </em>seed did not elicit any lethal signs of morbidity and mortality at doses up to 5000mg/Kgb.wt. and are therefore considered generally safe.</p> <p><strong>Conclusion:</strong> <em>P. americana </em>seed ethyl acetate fraction contains essential phytochemicals with useful phyto-medicinal and nutraceutical benefits. The implications of these findings are further discussed.</p> E. S. Asiwe C. U. Igwe K. M. E. Iheanacho I. O. Onyeocha V. A. Onwuliri ##submission.copyrightStatement## 2021-07-21 2021-07-21 10 17 10.9734/ajrb/2021/v8i430186