Asian Journal of Research in Biochemistry <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry</strong>&nbsp;<strong>(ISSN: 2582-0516)&nbsp;</strong>aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p style="text-align: justify;">This is an open-access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.</p> Asian Journal of Research in Biochemistry en-US Asian Journal of Research in Biochemistry 2582-0516 Antioxidant Activity of Croton zambesicus Muell. Arg Seed Extract <p>The present study was designed to investigate the antioxidant activity of <em>Croton zambesicus </em>Muell. Arg seed extract and fractions (Family: <em>Euphorbiaceae</em>). The antioxidant property was assessed by testing for DPPH free radical scavenging activity. Potential antioxidant activity was presented by methanolic extract. <em>C. zambesicus </em>showed high DPPH value (83.21 ± 0.05). Crude extract was fractionated using separation funnel, the five fractions were obtained are hexane, chloroform, ethyl acetate, n-butanol and water which represented antioxidant activities equal to 46.2 ± 0.1349, 45.1 ± 0.0116, 49.9 ± 0.0337, 40.45 ± 0.2290 and 62.7 ± 0.0006 respectively.</p> Manal A. Ibrahim Doha E. Fadlalla Hiba E. Elkhalifa Malak E. Ahmed Sahar Br Ahmed ##submission.copyrightStatement## 2022-12-29 2022-12-29 1 5 10.9734/ajrb/2022/v11i3-4216 Ex-vivo Antispasmodic and In vivo Anti Salmonella Potential of Rumex bequaertii Leaves Extracts <p><strong>Objective:</strong> The present study aimed at investigating the acute toxicity, the <em>ex-vivo</em> antispasmodic potential of aqueous and ethanolic <em>Rumex bequaertii </em>leaves extracts in isolated ileum fragment and antimicrobial activity of <em>Rumex bequaertii </em>ethanolic leaves extract in animal models infected with <em>Salmonella typhi</em>.</p> <p><strong>Methods:</strong> Acute toxicity using a single dose of ethanolic extract of <em>Rumex bequaertii</em> at 2000 mg/kg was administered to female rats and effects were observed during 14 days. Different cumulative concentrations of the aqueous and ethanolic extracts of <em>Rumex bequaertii </em>leaves were tested for spontaneous contractions and potassium chloride-induced contractions in rat ileum fragment. Different doses of the ethanolic extract of <em>Rumex bequaertii </em>leaves were tested for antidiarrheal activity in Wistar rats infected with <em>Salmonella typhi</em>.</p> <p><strong>Results:</strong> Rats given the single dose of <em>Rumex bequaertii </em>ethanolic extract showed no significant changes in body and organs weights compared to control rats. Administration of extracts at all tested concentrations resulted to inhibition of ileum contractions. Inhibition of spontaneous contractions of ileum fragment by aqueous extract ranging from 24.48 to 90.19 %, for 2.91, to 15.25 mg/mL, respectively, while ethanolic extract was from 42.00 to 83.72 %, for 0.99, to 5.66 mg/mL, respectively. Concerning potassium chloride-induced contraction, aqueous extract concentrations (from 2.91&nbsp;to 15.25 mg/mL) inhibited contractions from 31.46 to 67.23 %; the ethanolic extract (0.99 to 5.66 mg/mL) inhibited from 38.25, to 82.60 % and verapamil from 14.31 to 80.70 %, at 0.06&nbsp;to 0.34 mg/mL. After administration of ethanolic extract, all tested doses resulted to reduction of <em>Salmonella typhi</em> load in stools and blood, with activities being duration and dose dependent.</p> <p><strong>Conclusion: </strong>The lethal dose 50 of the <em>Rumex bequaertii </em>ethanol extract is greater than 2000 mg/kg. Aqueous and ethanolic leaves’ extracts of <em>Rumex bequaertii </em>possess <em>ex-vivo</em> antispasmodic and ethanolic leaves extract of <em>Rumex bequaertii </em>possess antimicrobial activity.</p> Claude Ruphin Mougoue Deumeni André Perfusion Amang Céline Nguefeu Nkenfou Roméo Georges Bonsou Fosin Augustin Notedji Roland Tchuenteu Tchuenguem Gael Tchokomeni Siwe Jules-Roger Kuiate Pierre Watcho Paul Vernyuy Tan ##submission.copyrightStatement## 2022-12-30 2022-12-30 6 14 10.9734/ajrb/2022/v11i3-4217