Asian Journal of Research in Biochemistry <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US (Asian Journal of Research in Biochemistry) (Asian Journal of Research in Biochemistry) Wed, 24 Mar 2021 10:43:49 +0000 OJS 60 Protective Potential of Lycopene Enriched Tomato Extract against Dexamethasone Induced Hepatic and Renal Damage in Mice <p><em></em></p> <p><strong>Aim:</strong> The present study was carried out to determine the protective potential of Lycopene enriched tomato extract (LycT) against hepatic and renal damage caused in mice by dexamethasone administration.</p> <p><strong>Study Design:</strong> Male LACA mice were randomly divided into four treatment groups (n=6-7 animals per group) depending upon the treatment they received. Group I (control) animals served as control and were orally administered with olive oil (vehicle) thrice a week for five weeks. Group II (DEX) animals were intraperitoneallly (i.p.) administered with dexamethasone at a dose of 5 mg/kg b.w. on alternate days for three weeks. Group III (LycT) animals were orally (p.o.) administered with LycT at a dose of 5 mg/kg b.w. on alternate days for five weeks. Group IV (LycT+DEX) animals were co-administered with LycT (p.o.) and dexamethasone (i.p.) according to the above mentioned dose regimen</p> <p><strong>Results: </strong>Dexamethasone caused hepatic and renal damage as evident from disturbed histoarchitecture, deranged levels of organ function markers (alkaline phosphatase, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, total and direct bilirubin, urea, creatinine) and enhanced level of cell damage (lactate dehydrogenase) and oxidative stress (lipid peroxidation) markers. Increased blood glucose level, decreased hepatic glycogen level along with inhibited activities of enzymes involved in glycolysis (hexokinase, phosphoglucoisomerase) indicated altered glucose metabolism in DEX group. The mitigation in histoarchitectural alterations, cell damage and oxidative stress markers, improved levels of organ function markers, blood glucose level along with ramped up antioxidant defense system indicated the protective potential of LycT against dexamethasone induced ill effects.</p> <p><strong>Conclusions:</strong> These results point towards beneficial effects of LycT against dexamethasone induced damage to hepatic and renal tissues in mice.</p> A. Koul, J. Kaur, N. A. Chugh ##submission.copyrightStatement## Wed, 24 Mar 2021 00:00:00 +0000 Study the Protective Effect of Ginger against the Toxicity of Dimethoate on Hormones in Rabbits <p>Organophosphorus insecticides have been widely classified as a health dangerous and high toxicity compounds due to their widespread use and release into the environment. Ginger <em>(Zingiber officinale</em>) has been used as a medicinal plant since antiquity and is known to play diverse biological roles including anti oxidation, anti-inflammation, hyperlipidemia, anti-carcinogenesis, anti-nausea, anti-thrombosis, and antibacterial process. The purpose of these experiments was to study decreasing the toxicity effect of Dimethoate by ginger. All animals in this study were assigned to one of four treatment groups: 0&nbsp;mg ginger and 0&nbsp;mg dimethoate /kg BW (control); 100&nbsp;mg ginger/kg BW; 43.2&nbsp;mg dimethoate /kg BW; and 43.2&nbsp;mg dimethoate plus 100&nbsp;mg ginger/kg BW. Rabbits were orally&nbsp;managed&nbsp;the&nbsp;particular&nbsp;measurements&nbsp;each&nbsp;other day for 12 weeks. Results indicated that treatment with ginger alone caused significant (P&lt;0.05) increase in body weight (BW) and relative weight of testes compared to control animals. Whereas the rabbits treated with dimethoate showed significant (P&lt;0.05) decrease in BW and relative weight of testes compared with control. Results showed that treatment with DM caused significant (P&lt;0.05) decrease activity of testosterone, T<sub>3</sub> and T<sub>4</sub>. While, increase the levels of FSH and LH in plasma. Ginger caused significant (P&lt;0.05) increase in the activity of testosterone, T<sub>3</sub> and T<sub>4</sub> in plasma compared to control. While, decrease the levels of FSH and LH in plasma. The presence of ginger with DM caused significant (P&lt;0.05) decrease in the reduction of T<sub>3</sub> and T<sub>4</sub>, while caused an improvement in the levels of testosterone as compared to control and the presence of ginger with DM caused increase in the levels of FSH and LH as compared to control, and this means that ginger counteracted the toxic effects of DM.</p> Sara M. Elgazwi, Fayrouz A. Khaled, Manal F. Alsanous ##submission.copyrightStatement## Thu, 01 Apr 2021 00:00:00 +0000 Toxicological Evaluation and Possible Reversal of Diabetic Toxicological Complications by PHF5 an Antidiabetic Herbal Formula in Wistar Albino Rats <p><strong>Aim:</strong> Medicinal potentials of herbs can be amplified greatly through synergism in a poly-herbal formulations. Toxic propensities of a plant can be masked out in poly-herbal preparations thus enhancing effectiveness. The aim of this study was to evaluate the potential toxicological effect and toxicity ameliorating properties of a poly-herbal formula (PHF5) prepared in a particular ratio from the leaves of <em>Ocimmum gratissimum, Vernonia amygdalina, Gongronema latifolium, Gnetum africanum </em>and <em>Aloe barbadensis </em>against alloxan-induced diabetic male rats.</p> <p><strong>Methods:</strong> Thirty-six (36) Wistar rats divided into 6 groups with 6 animals per group were used for this study. Group 1 served as normal control animals; group 2 had the diabetic rats treated with PHF5 (75 mg/kg bw); group 3, diabetic rats treated with PHF5 (150 mg/kg bw); group 4, diabetic rats treated with PHF5 (300 mg/kg bw); group 5, diabetic rats not given any intervention, group 6 diabetic animals treated with Glibenclamide (5 mg/kgbw). The induction of diabetes was done intraperitoneally using alloxan monohydrate (100 mg/kg bw). Administration of PHF5 was done orally for five weeks.</p> <p><strong>Results:</strong> Acute toxicity studies of PHF5 did not show any toxic symptoms in animals that received the PHF5 at up to 5000 mg/kg bw dose. The elevated liver (TP, AST, ALT and ALP) and kidney (BUN and Creatnine) markers in the diabetic animals were lowered significantly in the PHF5-treated animals.</p> <p><strong>Conclusion:</strong> The findings of this study suggests that PHF5 possess protective properties against hepatotoxicity and diabetic nephropathy and that the extract is quite safe for consumption</p> Egba Simeon Ikechukwu, N. Okafor Polycarp, E. Mbah Patricia, C. Ikechukwu Gavin, C. Omeoga Humphrey, W. Eze Chukwuka ##submission.copyrightStatement## Wed, 14 Apr 2021 00:00:00 +0000 Effects of Aqueous Stem Bark Extract of Stereospermum kunthianum on Carbon Tetrachloride - Induced Hepatotoxicity in Rats <p><strong>Aim: </strong>This study is aimed at evaluating the effects of the aqueous stem bark extract of <em>Stereospermum kunthianum</em> on CCl<sub>4</sub>-induced hepatotoxicity in rats.</p> <p><strong>Methodology: </strong>Analysis of qualitative phytochemical components and antioxidant activity were carried out. Experimental rats were randomly divided into six groups of five rats each. Group 1: served as the normal control group. Group 2: was administered with CCl<sub>4</sub> only at a dose of 3 ml/kg b.wt by single intraperitoneal administration. Group 3: served as the standard control group.&nbsp;&nbsp; Group 4: was administered with 200 mg/kg b.wt of the aqueous stem bark extract + CCl<sub>4</sub>. Group 5: was administered with 400 mg/kg b.wt of the aqueous stem bark extract + CCl<sub>4</sub>. Group 6: was administered with 600 mg/kg b.wt of the aqueous stem bark extract+ CCl<sub>4</sub>.</p> <p><strong>Results:</strong> The phytochemical analysis showed the presence of flavonoid, phenols, saponins, and terpenoid while tannins and alkaloids were absent. The antioxidant activity showed that the extract significantly (P&lt;0.05) inhibits Ferric Reducing Antioxidant Power (FRAP) and Thiobarbituric Acid Reactive Substances (TBARS), giving high activity as the concentration of the extract increases. The elevated levels of ALT and AST coupled with Conjugated bilirubin, Total bilirubin, and total protein caused by CCl<sub>4</sub> administration were all reduced significantly (P&lt;0.05) by the extract in dose dependent manner.</p> <p><strong>Conclusion:</strong> These findings demonstrated that stem bark extract of<em> Stereospernmum kunthianum</em> could be an alternative medication for liver injury.</p> Mahmoud Suleiman Jada, Mohammed Aliyu Sulaiman, Muktar Abdulmalik ##submission.copyrightStatement## Fri, 23 Apr 2021 00:00:00 +0000