Asian Journal of Research in Biochemistry https://journalajrb.com/index.php/AJRB <p style="text-align: justify;"><strong>Asian Journal of Research in Biochemistry&nbsp;(ISSN: 2582-0516)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/AJRB/general-guideline-for-authors">Click here for Types of paper</a>) in the field of Biochemistry. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US contact@journalajrb.com (Asian Journal of Research in Biochemistry) contact@journalajrb.com (Asian Journal of Research in Biochemistry) Mon, 04 Jan 2021 00:00:00 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Evaluation of Bactericidal and Fungicidal Efficacy of Strychno spotatorum Linn. (Nirmali) Seeds https://journalajrb.com/index.php/AJRB/article/view/30169 <p><strong>Aim: </strong>Diseases due to pathogenic microbes pose a great burden on human health and they have been correlated with socioeconomic, environmental, and ecological factors. The threat due to infectious diseases is further intensified by the continued emergence of new and multidrug resistant microorganisms. This scenario warrants a continuous search for antimicrobial agents preferably of plant origin due to their availability, accessibility, and affordability. The present study was aimed to evaluate the antibacterial and antifungal properties of <em>Strychnos potatorum Linn </em>(Nirmali) seeds using common pathogenic bacterial and fungal strains.</p> <p><strong>Methodology: </strong>Fresh and matured <em>S. potatorum</em> seeds were used for the present study. The powdered seeds were delipidated with petroleum ether (60-80°C) overnight and the extract was filtered. Soxhalation was performed with 95% ethanol to extract the phyto-ingredients from the seeds. Four Gram positive, four Gram negative, and eight fungal strains were used. The antimicrobial activity was evaluated by disc diffusion and well diffusion methods. The Minimum inhibitory concentration (MIC) Minimum bactericidal concentrations (MBC), Minimum fungicidal concentration (MFC) were assayed.</p> <p><strong>Results: </strong>The data obtained through the disc diffusion, well diffusion, the minimum bactericidal concentration, and minimum fungicidal concentrations revealed that the ethanolic extract of the seeds possesses significant antibacterial and antifungal activities. The results obtained were compared with standard drugs widely prescribed for antimicrobial therapy.</p> <p><strong>Conclusion: </strong>The present study provides the scientific rationale for the use of <em>Strychnos potatorum</em> seeds in traditional medicine and a rich source of phytochemicals having significant antimicrobial activities.</p> Chandiran Sharmila, Rajendiran Selvam, Sorimuthu Pillai Subramanian ##submission.copyrightStatement## https://journalajrb.com/index.php/AJRB/article/view/30169 Mon, 04 Jan 2021 00:00:00 +0000 Comparative Analysis of Nutrients Content and Characterization of Oil from Two Varieties of Tiger Nut (Cyperus esculentus) https://journalajrb.com/index.php/AJRB/article/view/30170 <p><strong>Background:</strong> Tiger nut (<em>Cyperus esculentus</em>) belong to the family of Cyperaceae and the order of Commelinalis. It has been existing for more than 4,000 years ago.</p> <p><strong>Objective: </strong>To determine the nutritional composition (protein, fat, fiber, ash, moisture and carbohydrate) of nut, to extract and characterize oil from varieties of tiger nuts, to determine the mineral elements presence in the nut.</p> <p><strong>Study Design: </strong>A descriptive research design was adopted by this study to determine the nutritional composition (protein, fat, fiber, ash, moisture and carbohydrate) of nut, to extract and characterize oil from varieties of tiger nuts and to determine the mineral elements presence in the nut.</p> <p><strong>Place and Duration of the Study:</strong> The study was conducted at Biochemistry Department, Bayero University Kano, between April, 2019 to September, 2019.</p> <p><strong>Methods:</strong> The Proximate compositions were determined using the method describe by Association of official analytical chemist’s, while Carbohydrate were determined by difference. The physicochemical properties were determined using the method describe by American oil Chemist’s society and Mineral composition were determined.</p> <p><strong>Results: </strong>The proximate composition of the yellow variety was moisture (9.48%), ash (2.07%), fat (33.5%), protein (6.11%), crude fibre (17.5%) and carbohydrate (31.2%). Corresponding values of the brown variety was moisture (9.62%), ash (2.76%), fat (34.2%), protein (6.93%), crude fibre (15.3%) and carbohydrate (30.9%) respectively. The extracted oil has a golden colour and a nutty taste. The saponification, acid, peroxide, iodine and free fatty acid values of the yellow variety were found to be 210.8±4.28, 3.17±0.64, 1.00±0.52, 78.7±13.1 and 0.40±0.21 and the saponification, acid, peroxide, iodine and free fatty acid values of the yellow variety were also found to be 212.2±4.92, 3.36±0.56, 1.06±0.75, 76.5±14.6 and 0.42±0.04 and was not significantly (P&gt; 0.05) different between th yellow and brown varieties respectively. The mineral element (mg/100g) of the brown variety is Mg 133.67, P 527.33, K 957.67, Ca 394, Cu 2.0 and Fe 1.86. Corresponding values for the yellow variety are Mg 118.13, P 159.61, K 384.33, Ca 152, Cu 2.0 and Fe 1.04. Lead and Cadmium were not detected in both varieties.</p> <p><strong>Conclusion: </strong>These results indicate that tiger nut tuber oil could be a good source of edible oil, highly nutritive and can provide a lot of energy like some starchy food.</p> A. M. Gadanya, M. Y. Abubakar, F. U. Maigari, L. Mudassir, S. M. Abubakar ##submission.copyrightStatement## https://journalajrb.com/index.php/AJRB/article/view/30170 Tue, 05 Jan 2021 00:00:00 +0000 Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy https://journalajrb.com/index.php/AJRB/article/view/30171 <p><em>Tamarindus indica</em> is one of the medicinal plants used in the treatment of various diseases traditionally.</p> <p><strong>Aims: </strong>This study was conducted to identify the phytochemical constituents of <em>T. indica</em> leaf extracts. <strong>Methods:</strong> Using Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) to identification of bioactive compounds in extracts of <em>T. indica</em>.</p> <p><strong>Results:</strong> The FT-IR spectrum confirmed the presence of alcohol group, alkene group, amine group, carbonates, ethers, carboxylic acid and disulfides in both extracts.</p> <p>A total of 22 and 38 bioactive phytochemical compounds were identified in the ethanolic and aqueous extracts of <em>T. indica,</em> respectively. The major bioactive compounds of the ethanolic extract of <em>T. indica</em> leaves were cis-Vaccenic acid, trans-13-Octadecenoic acid, Oleic Acid, Octadecanoic acid, Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester, Eicosanoic acid and Eicosane, 1-Iodo-2-methylundecane, 10-Methylnonadecane. While the major bioactive compounds of the aqueous extract were 3-O-Methyl-d-glucose, Myo-Inositol, 4C-methyl-, Myo-Inositol, 2-C-methyl-, Propane, 2,2-dimethoxy-, 1,3-Dioxolane, Ethanol, 2-(1-methylethoxy)-, and 2-Pentanone, 4-hydroxy-4-methyl-, 2-Hexanol, 2-methyl-, Ethanamine, N-methyl.</p> Mansour Abdulnabi H. Mehdi, Abdul-hakim M. A. Al- Alawi, Ahmed Zain A. Thabet, Fadel Y. S. Alarabi, Gozif Mohammed N. Omar, Vidya Pradhan ##submission.copyrightStatement## https://journalajrb.com/index.php/AJRB/article/view/30171 Thu, 14 Jan 2021 00:00:00 +0000