Open Access Original Research Article

Protective Potential of Lycopene Enriched Tomato Extract against Dexamethasone Induced Hepatic and Renal Damage in Mice

A. Koul, J. Kaur, N. A. Chugh

Asian Journal of Research in Biochemistry, Page 1-23
DOI: 10.9734/ajrb/2021/v8i330180

Aim: The present study was carried out to determine the protective potential of Lycopene enriched tomato extract (LycT) against hepatic and renal damage caused in mice by dexamethasone administration.

Study Design: Male LACA mice were randomly divided into four treatment groups (n=6-7 animals per group) depending upon the treatment they received. Group I (control) animals served as control and were orally administered with olive oil (vehicle) thrice a week for five weeks. Group II (DEX) animals were intraperitoneallly (i.p.) administered with dexamethasone at a dose of 5 mg/kg b.w. on alternate days for three weeks. Group III (LycT) animals were orally (p.o.) administered with LycT at a dose of 5 mg/kg b.w. on alternate days for five weeks. Group IV (LycT+DEX) animals were co-administered with LycT (p.o.) and dexamethasone (i.p.) according to the above mentioned dose regimen

Results: Dexamethasone caused hepatic and renal damage as evident from disturbed histoarchitecture, deranged levels of organ function markers (alkaline phosphatase, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, total and direct bilirubin, urea, creatinine) and enhanced level of cell damage (lactate dehydrogenase) and oxidative stress (lipid peroxidation) markers. Increased blood glucose level, decreased hepatic glycogen level along with inhibited activities of enzymes involved in glycolysis (hexokinase, phosphoglucoisomerase) indicated altered glucose metabolism in DEX group. The mitigation in histoarchitectural alterations, cell damage and oxidative stress markers, improved levels of organ function markers, blood glucose level along with ramped up antioxidant defense system indicated the protective potential of LycT against dexamethasone induced ill effects.

Conclusions: These results point towards beneficial effects of LycT against dexamethasone induced damage to hepatic and renal tissues in mice.

Open Access Original Research Article

Study the Protective Effect of Ginger against the Toxicity of Dimethoate on Hormones in Rabbits

Sara M. Elgazwi, Fayrouz A. Khaled, Manal F. Alsanous

Asian Journal of Research in Biochemistry, Page 24-33
DOI: 10.9734/ajrb/2021/v8i330181

Organophosphorus insecticides have been widely classified as a health dangerous and high toxicity compounds due to their widespread use and release into the environment. Ginger (Zingiber officinale) has been used as a medicinal plant since antiquity and is known to play diverse biological roles including anti oxidation, anti-inflammation, hyperlipidemia, anti-carcinogenesis, anti-nausea, anti-thrombosis, and antibacterial process. The purpose of these experiments was to study decreasing the toxicity effect of Dimethoate by ginger. All animals in this study were assigned to one of four treatment groups: 0 mg ginger and 0 mg dimethoate /kg BW (control); 100 mg ginger/kg BW; 43.2 mg dimethoate /kg BW; and 43.2 mg dimethoate plus 100 mg ginger/kg BW. Rabbits were orally managed the particular measurements each other day for 12 weeks. Results indicated that treatment with ginger alone caused significant (P<0.05) increase in body weight (BW) and relative weight of testes compared to control animals. Whereas the rabbits treated with dimethoate showed significant (P<0.05) decrease in BW and relative weight of testes compared with control. Results showed that treatment with DM caused significant (P<0.05) decrease activity of testosterone, T3 and T4. While, increase the levels of FSH and LH in plasma. Ginger caused significant (P<0.05) increase in the activity of testosterone, T3 and T4 in plasma compared to control. While, decrease the levels of FSH and LH in plasma. The presence of ginger with DM caused significant (P<0.05) decrease in the reduction of T3 and T4, while caused an improvement in the levels of testosterone as compared to control and the presence of ginger with DM caused increase in the levels of FSH and LH as compared to control, and this means that ginger counteracted the toxic effects of DM.