Open Access Original Research Article

Quantitative Determination of Heavy Metal Concentrations in Herbal Teas Marketed in Various Countries Including Libya

M. A. Elbagermi, A. I. Alajtal, H. G. M. Edwards

Asian Journal of Research in Biochemistry, Page 1-10
DOI: 10.9734/ajrb/2017/v1i1319

This study presents the determination of the amount of some heavy metals (Cu, Cr, Ni, Cd, Mn, Fe, Mg and Pb) present in commercial brand herbal tea samples purchased from local markets in Misurata, Libya, by atomic absorption spectroscopy. The validity of the analytical procedure was monitored by analysing certified reference materials obtained from the Food and Drugs Control Centre, Libya. The concentration of Cu, Cr, Ni, Cd, Mn, Fe, Mg and Pb in all the tea leaf samples ranged from 5.141 to 17.1, 0.890 to 3.4, 0.0833 to 2.349, 0.035 to 0.38, 32.01to 89.46, 79.01-167, 91.98 to 213.83 and 0.463 to 0.901 µg g-1, respectively. The concentration of heavy metals in the tea leaves can be arranged in the following order, Mg> Fe> Mn>Cu >Cr> Ni> Pb> Cd. Moreover, it is observed that the concentrations of all the toxic elements tested in the investigated herbal plants are found below the permitted levels specified by the international regulatory standards for the medicinal plants.

Open Access Original Research Article

Comparative Study of Mineral and Phytochemical Analysis of Soil and Lactuca sativa Grown in the Vicinity of Cement Company of Northern Nigeria (Sokoto Cement) and Usmanu Danfodiyo University Sokoto (Kwalkwalawa)

M. H. Dalhat, A. R. Amale, M. Maimuna, I. Bashiru, K. Sirajo

Asian Journal of Research in Biochemistry, Page 1-10
DOI: 10.9734/ajrb/2017/v1i1329

Environmental pollution is a major issue which confronts industry and business in today’s world on daily basis. Industrial activities are the leading cause of metals emission, often associated with soil and plant metal concentration in adjacent regions. Cement industry is one of the 17 most polluting industries listed by the central pollution control board (CPCB). Impact of dust deposition from Cement Company of Northern Nigeria on the proximate and phytochemical concentrations of lettuce (Lactuca sativa) was studied. A comparative study of heavy metal concentration and phytochemicals of Lactuca sativa and soil samples from Kalambaina (Industrial area) and Kwalkwalawa (non-Industrial area) were estimated using atomic absorption spectrophotometry (AAS) and standard analytical procedures respectively. Result of quantitative phytochemical analysis revealed significant difference (P<0.05) in all parameters. Heavy metal values of Pb(0.012±0.002 mg/g), Zn(0.043±0.003 mg/g), and Ca(706.860±14.980 mg/g) in Lactuca sativa collected from Kalambaina revealed significant difference (P<0.05) when compare to samples collected from Kwalkwalawa and WHO standard. In addition, the heavy metal concentration in soil collected from Kalambaina showed significant difference (P<0.05) when compare to samples collected from Kwalkwalawa; with the highest value recorded in Ca (974.25±48 mg/g) which might be as a result of activities in the cement industry. Conclusively, plants grown at cement industries might not be safe for consumption

Open Access Original Research Article

Toxicological Evaluation of Two Named Herbal Remedies Sold Across Orumba South Local Government Area of Anambra State, South-Eastern Nigeria

S. I. Egba, J. O. Ogbodo, P. O. Ogbodo, C. A. Obike

Asian Journal of Research in Biochemistry, Page 1-6
DOI: 10.9734/ajrb/2017/v1i1355

Aim: Herbs are plants or parts of plants used for their therapeutic, aromatic or savoury values. This work studied the potential sub-chronic toxic effects of Goko and BetaB, two herbal remedies used in treating human diseases and sold in Orumba Local Government Area of Anambra state, Nigeria.

Design: Experimental adult Wister female albino rats were divided into five groups (A, B, C, D and E) of five animals per group. The first and second groups received 0.1 ml/kg body weight and 0.2 ml/kg body weight of Goko while the third and fourth groups received 0.1 ml/kg body weight and 0.2 ml/kg body weight of BetaB orally. The control group was given standard feed and clean drinking water only. Administration lasted for 14 days after which the animals were sacrificed by cervical dislocation and blood samples collected for biochemical assay.

Results: The results of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity and concentration of serum total bilirubin and albumin showed varying significant (P < 0.05) differences when compared with the control.

Conclusion: Result obtained from this study seems to suggest that Goko and BetaB may not be safe for use sub-chronically at high doses.

Open Access Original Research Article

Effects of Ethanol Leaf and Fruit Extracts of Kigelia africana on Some Oxidative and Biochemical Parameters of Alloxan -Induced Diabetic Rats

E. N. Uhuo, L. U. S. Ezeanyika, V. N. Ogugua

Asian Journal of Research in Biochemistry, Page 1-17
DOI: 10.9734/ajrb/2017/v1i1366

Hyperglycaemia, a characteristic feature of diabetics mellitus leads to decreased antioxidant defense and hence the development of oxidative stress, which is involved in the aetiology of development of diabetic complications. This work was therefore aimed at evaluating the anti diabetic and antioxidative potential of the plant. These evidences suggest that good glycemic control and/or use of antioxidants may play an important role in the prevention of complications associated with diabetes. Diabetes was induced with single Intra peritoneal injection of alloxan (160 mg/kg b.w) dissolved in freshly prepared citrate buffer (pH 4.5). Oral administration of Kingelia africana (500 mg/kg b.wt) of methanol leaves and fruits extracts resulted in significant (p>0.05) decrease in the blood glucose level, MDA, glycosylated haemoglobin, lipid profiles and  liver maker enzymes with corresponding increase in SOD activity, catalase activity, glutathione activity, serum protein concentration, and Vit.C concentration. In conclusion, K. africana possessed antioxidative properties evidenced by decrease blood glucose level and its effect on some oxidative parameters of diabetic rats.