Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy
Mansour Abdulnabi H. Mehdi *
Department of Zoology, Dr. Rafiq Zakaria College for Women, Aurangabad, 431001, India. and Department of Biology, Faculty of Education-Rdfan, Aden University, Aden, Yemen.
Abdul-hakim M. A. Al- Alawi
Department of Biology, Faculty of Education-Rdfan, Aden University, Aden, Yemen.
Ahmed Zain A. Thabet
Department of Chemistry, Maulana Azad College of Art, Science and Commerce, Aurangabad, India.
Fadel Y. S. Alarabi
Department of Zoology, Dr. Rafiq Zakaria College for Women, Aurangabad, 431001, India. and Department of Biology, Faculty of Education-Rdfan, Aden University, Aden, Yemen.
Gozif Mohammed N. Omar
Department of Biology, Faculty of Education-Rdfan, Aden University, Aden, Yemen. and Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, 431001, India.
Vidya Pradhan
Department of Zoology, Dr. Rafiq Zakaria College for Women, Aurangabad, 431001, India.
*Author to whom correspondence should be addressed.
Abstract
Tamarindus indica is one of the medicinal plants used in the treatment of various diseases traditionally.
Aims: This study was conducted to identify the phytochemical constituents of T. indica leaf extracts. Methods: Using Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) to identification of bioactive compounds in extracts of T. indica.
Results: The FT-IR spectrum confirmed the presence of alcohol group, alkene group, amine group, carbonates, ethers, carboxylic acid and disulfides in both extracts.
A total of 22 and 38 bioactive phytochemical compounds were identified in the ethanolic and aqueous extracts of T. indica, respectively. The major bioactive compounds of the ethanolic extract of T. indica leaves were cis-Vaccenic acid, trans-13-Octadecenoic acid, Oleic Acid, Octadecanoic acid, Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester, Eicosanoic acid and Eicosane, 1-Iodo-2-methylundecane, 10-Methylnonadecane. While the major bioactive compounds of the aqueous extract were 3-O-Methyl-d-glucose, Myo-Inositol, 4C-methyl-, Myo-Inositol, 2-C-methyl-, Propane, 2,2-dimethoxy-, 1,3-Dioxolane, Ethanol, 2-(1-methylethoxy)-, and 2-Pentanone, 4-hydroxy-4-methyl-, 2-Hexanol, 2-methyl-, Ethanamine, N-methyl.
Keywords: Tamarindus indica, bioactive chemical compounds, FT-IR, GC-MS.